Your browser doesn't support javascript.
loading
Montrer: 20 | 50 | 100
Résultats 1 - 2 de 2
Filtre
Ajouter des filtres








Gamme d'année
1.
Rev. Soc. Bras. Med. Trop ; 46(3): 373-376, May-Jun/2013. tab, graf
Article Dans Anglais | LILACS | ID: lil-679532

Résumé

Introduction Neurotoxoplasmosis (NT) sometimes manifests unusual characteristics. Methods We analyzed 85 patients with NT and AIDS according to clinical, cerebrospinal fluid, cranial magnetic resonance, and polymerase chain reaction (PCR) characteristics. Results In 8.5%, focal neurological deficits were absent and 16.4% had single cerebral lesions. Increased sensitivity of PCR for Toxoplasma gondii DNA in the central nervous system was associated with pleocytosis and presence of >4 encephalic lesions. Conclusions Patients with NT may present without focal neurological deficit and NT may occur with presence of a single cerebral lesion. Greater numbers of lesions and greater cellularity in cerebrospinal fluid improve the sensitivity of PCR to T gondii. .


Sujets)
Adulte , Femelle , Humains , Mâle , Infections opportunistes liées au SIDA/diagnostic , Toxoplasmose cérébrale/diagnostic , Infections opportunistes liées au SIDA/liquide cérébrospinal , Études transversales , ADN des protozoaires/liquide cérébrospinal , Imagerie par résonance magnétique , Réaction de polymérisation en chaîne , Sensibilité et spécificité , Toxoplasmose cérébrale/liquide cérébrospinal
2.
Braz. j. infect. dis ; 13(1): 18-23, Feb. 2009. tab
Article Dans Anglais | LILACS | ID: lil-517810

Résumé

Encephalitis caused by Toxoplasma gondii is the most common cause of central nervous system damage in patients with acquired immunodeficiency syndrome (AIDS). Toxoplasma may infect any of the brain cells, thus leading to non-specific neurotoxoplasmosis clinical manifestations including focused or non-focused signs and symptoms of central nervous system malfunction. Clinical development ranges from insidious display during weeks to experiencing acute general confusion or ultimately fatal onset. Cerebral toxoplasmosis occurs in advanced stages of immunodeficiency, and the absence of anti-toxoplasmosis antibodies by the immunofluorescence method does not allow us to rule out its diagnosis. As specific therapy begins, diagnosis confirmation is sought through clinical and radiological response. There are few accurate diagnosis methods to confirm such cases. We present a method for T. gondii DNA detection by real time PCR-Multiplex. Fifty-one patients were evaluated; 16 patients had AIDS and a presumptive diagnosis for toxoplasmosis, 23 patients were HIV-positive with further morbidities except neurotoxoplasmosis, and 12 subjects were HIV-negative control patients. Real time PCR-Multiplex was applied to these patients' cephalorachidian liquid with a specific T. gondii genome sequence from the 529bp fragment. This test is usually carried out within four hours. Test sensitivity, specificity, positive predictive value, and negative predictive value were calculated according to applicable tables. Toxoplasma gondii assay by real time Multiplex of cephalorachidian fluid was positive for 11 out of 16 patients with AIDS and a presumptive diagnosis for cerebral toxoplasmosis, while none of the 35 control patients displayed such a result. Therefore, this method allowed us to achieve 68.8 percent sensitivity, 100 percent specificity, 100 percent positive predictive value, and 87.8 percent negative predictive value. Real time PCR on CSF allowed high specificity...


Sujets)
Adulte , Animaux , Femelle , Humains , Mâle , Adulte d'âge moyen , Infections opportunistes liées au SIDA/diagnostic , ADN des protozoaires/liquide cérébrospinal , Réaction de polymérisation en chaîne/méthodes , Toxoplasma/génétique , Toxoplasmose cérébrale/diagnostic , Infections opportunistes liées au SIDA/liquide cérébrospinal , Études cas-témoins , Valeur prédictive des tests , Sensibilité et spécificité , Toxoplasmose cérébrale/liquide cérébrospinal
SÉLECTION CITATIONS
Détails de la recherche