Clinical evaluation of herbal coded formulation cran-off to urixin in the treatment of urinary tract infection

Urinary Tract Infections are the largest group of infections after the respiratory tract infections. In 85% of the cases the causative organism is E. Coli. A clinical trial was conducted to evaluate the efficacy of coded herbal formulation [Cran off] [Test drug] for the treatment of Urinary tract infection comparing with Urixin [Control]. One hundred and thirty patients suffering from Urinary tract infection from both groups [Males: 45, mean age: 34+/-14 and females: 85, mean age: 33+/-13 year, range: 15-55] were enrolled in the trial and divided in to two groups according to treatment regimens. Cran off [Test drug] 500mg two capsules and Urixin [Pipemidic Acid Trihydrate JP15] [Control] 400mg capsules twice daily were prescribed for 2-3 weeks. Urinary tract infection was improved in 23 [35.38%] patients out of 65 patients by the use of Cran off [Test drug], and in 15 [23.07%] patients out of 65 by the use of Urixin [Control drug]. Furthermore, there was a significant improvement in Urinary tract infection associated clinical features as compared to Urixin. It is concluded that Cran off possesses a therapeutic value for the improvement of urinary tract infection and its associated symptoms as compared to Urixin

Study on the molecular mechanism of quinolone resistance in Shigellae spp / 中华流行病学杂志

<p><b>OBJECTIVE</b>To study the resistance and its mechanism of Shigellae spp. to quinolones.</p><p><b>METHODS</b>Seventy-three clinical isolates were collected. Susceptibility tests of pipemidic adcid (PI), ofloxacin (OFL), norfloxacin (NOR), and ciprofloxacin (CIP) were performed in all clinical isolates and Shigella 51573. The N-terminal coding region of gyrA and parC were amplified by polymerase chain reaction (PCR) respectively. Restriction fragment length polymorphism (RFLP) was applied to all PCR procucts of gyrA and parC, and single strand conformational polymorphism analysis (SSCP) was also applied to PCR procucts of parC.</p><p><b>RESULTS</b>The resistance rates for all the Shigella spp. to PI, CIP, NOR and OFL were 79.5%, 60.3%, 41.1% and 36.9%. Sixty-seven strains (91.8%) were quinolone-reduced-sensitive isolates, in which 61 strains (91%) were found carrying mutations in gyrA with 5 strains (7.5%) found carrying mutations in parC. No mutation was found in 6 quinolone-sensitive isolates or Shigella 51573.</p><p><b>CONCLUSION</b>The Shigella spp. had high resistance rates to quinolones. The target gene mutations which were mainly found in gyrA and secondarily in parC, played an important role in the quinolone-resistance in Shigella spp.</p>

Genotoxic evaluation of Norfloxacin and Pipemidic acid with the Eschirichia coli PolA-/polA+ and the ames test

Background. Genotoxicity of antibiotic has not been well evaluted, and there is not much information on the genetic risk of quinolone drugs, even though they are widely used as alternative choice drugs in urinary infections. Methods. Pipemidic acid and norfloxacin were tested for their capacity to induce point mutations using the Ames test and DNA damage on Escherichia coli PolA-/PolA+. Results. At non-toxic doses, all of the drugs studied were negative on the E. coli PolA-/PolA+ test with or without in vitro metabolic activation with induced arochlor 1254 rat liver (S9). They did not procedure frameshift mutations in TA98, or base-air substitutions in S. typhimurium hisG46 strains TA100, or UTH8414. Norfloxacin and its induced metabolites in vitro with S9 rat liver were mutagenic to hisG48 strains TA102 and TA104, both of which detect oxidative chemicals. Pipemidic acid induced mutations in S. typhimurium hisG48 strains only when they had an efficient DNA excision repair system. Conclusions. These results suggest that the risk of oxygen-free radical generation from quinolones should be considered

Padräo de resistência a quinolonas de amostras multiresistentes de Staphylococcus aureus de origem hospitalar / The resistance pattern to quinolones with 12 staphylococcus aureus multiresistance samples from hospitals

Foi realizado um estudo do padräo de resistência às quinolonas em amostras de staphylococcus aureus oxacilina resistentes (ORSA) isoladas de pacientes internos no Hospital Universitário "Lauro Wanderley" (HU/UFPb). Utilizando 12 amostras bacterianas caracterizadas fenotipicamente em termos de identificaçäo, sorologia, triagem em agar e teste de sensibilidade aos microbianos utilizando-se as seguintes quinolonas: ácidos nalidíxico, ácido pipemídico, norfloxacin, pefloxacin, ciprofloxacin, lomefloxacin e ofloxacin. Os resultados demonstraram que das 12 amostras de ORSA a maioria foi proveniente da Clínica Cirúrgica 08/12 (66,8 por cento), e relacionando o isolamento bacteriano ao sítio anatômico verificou-se que houve a predominância de amostras clínicas provenientes de ferida cirúrgica 07/12 (58,3 por cento). Com relaçäo ao teste de sensibilidade às drogas, verificou-se uma média de resistência de 53,0 por cento. Entre as quinolonas testadas às quais as bactérias apresentaram maior índice de sensibilidade destacam-se: Oflaxacin (83,3 por cento), Ciprofloxacin e Lomefloxacin (75,0 por cento), e Pefloxacin (58,3 por cento). Enquanto as que apresentaram menor eficácia foram: Nalidíxico (8,4 por cento) e Pipemídico (16,7 por cento)

Sensibilidad a antimicrobianos comunes de bacterias obtenidas en urocultivos positivos de niños / Common antimicrobial susceptibility of bacteria from positive urine cultures of children

Se describe la frecuencia relativa de las bacterias patógenas y su sensibilidad a los antimicrobianos en 400 urocultivos sucesivos con recuentos de colonias * 10 elevado a 5 de personas de 15 o menos años de edad, ambulatorias (n: 216) y hospitalizadas, de ambos sexos (300 niñas). Escherichia coli fue el agente aislado con mayor frecuencia (75,7% del total, 89,1% en niñas y 58,5% en varones ambulatorios, 76,8% en niñas y 45,7% en niños hospitalizados), seguido de klebsiella pneumoniae en niñas (3,5%) y proteus mirabilis en varones (22,0%) ambulatorios. En pacientes del hospital, klebsiella pneumoniae y klebsiella species fueron segundas en frecuencia en niñas (13,4%) y varones (18,5%). Pseudomonas aeruginosa sólo se detectó en pacientes del hospital (10,1% varones, 1,6% niñas). Un tercio de las cepas de E. coli eran resistentes a cefalosporinas de primera generación, más de la mitad a ampicilina y cotrimoxazol, siendo la mayoría (*96%) sensibles a gentamicina y nitrofurantoína. En las muestras de niños hospitalizados, 28% E. coli eran resistentes a ampicilina-sulbactam (p 0,0000053), más de 40% a cefalosporinas de primera generación y más de 50% a cotrimoxazol y ampicilina, mientras 95% o más eran sensibles a amikacina y gentamicina (p 0,000). K. pneumoniae fueron casi siempre sensibles a amikacina (93,7%), pero sólo 20% a gentamicina (p 0,000148), siendo alrededor de la mitad sensibles a nitrofurantoína. Seis de siete cepas de pseudomonas aeruginosa eran sensibles a ceftazidima. A menos que estudios locales muestren una situación diferente, parece razonable sugerir que pacientes ambulatorios con infecciones urinarias aparentemente bajas sean tratados inicialmente de preferencia con nitrofurantoína y se prefiera amikacina si se sospecha infección alta, modificando posteriormente la conducta, si es conveniente, según el análisis de sensibilidad

Infecçäo pós-ressecçäo endoscópica de próstata: Estudo com ácido pipemídico / Prosthatetic endoscopic infection post-resection: A trial with pipemidic acid

Trinta e cinco pacientes, com idades de 45 a 86 anos, submetidos à cirurgia endoscópica de próstata e sondados por um período médio de dois dias no pós-operatório, receberam ácido pipemídico por via oral na dosagem diária de 800 mg, divididos em doses de 400 mg a cada 12 horas. O período de utilizaçäo da droga foi de oito dias. Foram realizadas uroculturas antes da intervençäo e, no mínimo, três dias após o termino do tratamento antimicrobiano. Consideraram-se infectadas todas as amostras de urina que apresentavam crescimento bacteriano, independente do número de colônias. Dos 35 pacientes estudos, 22 näo apresentaram infecçäo pós-operatória (63 por cento). De 26 pacientes sem infecçäo antes da operaçäo, 18 se mantiveram estéreis (69 por cento); dos nove previamente infectados, quatro se curaram após a ressecçäo (44 por cento). Considerando a praticidade posológica, a tolerabilidade e o baixo custo do ácido pipemídico, os autores concluem pela viabilidade da sua utilizaçäo em pacientes submetidos à ressecçäo endoscópica da próstata sem infecçäo prévia, sendo preferível, naqueles previamente infectados, o uso de antibióticos específicos, conforme orientaçäo do antibiograma

Antimicrobial activity of erythromycin, doxycycline, pipemidic acid, and enoxacine against chlamydia trachomatis / 감염

No abstract available.

Antimicrobial activity of erythromycin, doxycycline, pipemidic acid, and enoxacine against chlamydia trachomatis / 감염

No abstract available.

A Clinical Study of Benign Bone Tumors Treated by Partly Deproteinised Xenograft(Kiel Bone) / 대한정형외과학회잡지

The use of lyophilised bone from calves as a heterograft was introduced by Maatz and Bauermeister in 1957 as an alternative to autografts and freeze-dried homografts which were then in general use. The Kiel bone was known to possess no antigenecity and serial radiographs revealed that it is incorporated into the recipient site by a process of creeping substitution from surrounding bone. In patients with benign bone tumors, the surgical dead space was filled with Kiel bone graft in the departments of orthopedic surgery, Pusan national university hospital. The results were followed up for more than 14 months, and were evaluated by the palin film findings. The results were as follows : l. Of the 22 cases, satisfactory result was obtained in 18 cases(81,8%). 2. The mean lesion size(diameter of lesion/diameter of bone)of success cases was 61.6%, and the mean lesion size of failed cases was 82.8%. 3. The duration from the Kiel bone graft to the evidence of new bone formation and remodelling was 10.5 months on an average with a range of 7months to 15months, and the duratin from the Kiel bone graft to the complete resorption of the Kiel bone was 45.7 months on an average with a range of 29 months to 66 months. 4. The causes of the failed cases were thought to be incomplete removal of the lesion, decreased vascularity due to repeated surgery, large size of the lesion, and the potentiality of tumor, 5. It was difficult to evaluate success rate according to underlying disease because the number of the cases was not large enough, but giant cell tumor showed high recurrence rate.

Açäo do ácido pipemídico em infecçöes otorrinolaringofaríngicas / Mechanism of pipemidic acid in otorhinolaryngological infections

Foram tratados neste estudo 30 doentes portadores de quadros infecciosos otorrinolaringofaríngicos com o PPA, por via oral, na dose de 400 mg a cada 12 horas, durante 10 dias. Os resultados globais obtidos expressam que houve uma remissäo completa dos sinais e sintomas em 21 doentes, sendo que em 7 casos a remissäo parcial dos sinais e dos sintomas justificou prosseguimento terapêutico. O índice global de efetividade terapêutica revelado neste estudo foi, portanto, de 93%

Norfloxacina y acido pipemídico en infecciones urinarias / Norfloxacin and pipemidic acid in urinary infections

Se estudiaron 40 pacientes de infecciones urinarias aguda y crônicas. A un grupo de 20 pacientes se administró norfloxacina, 800 mg diarios por via oral durante 10 dias. Y a otro grupo de 20 pacientes se administró ácido pipemídico, 800 mg diarios por vía oral durante 10 días. En ambos grupos desapareció la sintomatologia en todos los pacientes. En el grupo de norfloxacina con la tinción de Gram se obtuvo 77,8% de resultados positivos. Y en el grupo de ácido pipemídico la tinción de Gram mostró 58,8% de resultados posistivos

Viabilidad y multiplicación de Listeria monocytogenes cepa murray frente a antimicrobianos de uso en medios selectivos / Viability and multiplication of Listeria monocytogenes strain Murray facing antimicrobial agents used in selective media

Se han efectuado estudios comparativos de velocidad de crecimiento, cosecha máxima y duración de la fase de adaptación de poblaciones de Listeria monocytogenes (CCMA 29454, ATCC 15313) a 37-C, en medios de cultivo líquidos conteniendo concentraciones variables de antimicrobianos con diferente espectro de acción. Concentraciones de 160microng/ml de ácido nalidíxico, 80microng/ml de ácido pipemédico, 40microng/ml de ácido oxolínico, 0,8U/ml de bacitracina y 200U/ml de nistatina, constituyen límites confiables que permiten la sobrevivencia y multiplicación de Listeria monocytogenes y ejercen una importante acción inhibitoria sobre el crecimiento de gran parte de la flora habitual de materiales normalmente sépticos. Es de importancia la potencial capacidad selectiva de diversas asociaciones de los agentes ensayados en la formulación de medios de cultivo destinados a un enriquecimiento de Listeria monocytogenes, que posibilite su aislamiento a partir de cultivos mixtos

Actividad antimicrobiana comparativa in vitro de norfloxacina, contra 200 cepas aisladas de urocultivos / Comparative antimicrobial activity in vitro of norfloxacin against 200 strains isolated from urocultures

La actividad in vitro de norfloxacina fue comparada con la del ácido nalidíxico, ácido pipemídico y cotrimoxazol, en contra de 200 bacilos gramnegativos (BGN) patógenos aislados por medio de urocultivos. Norfloxacina mostró ser más activa que los otros antibióticos estudiados. La concentración inhibitoria mínima (CIM) de norfloxacina para el 100% de E. coli fue de 0.5 mcg/ml; el 100% de P. mirabilis se inhibió con 0.25 mcg/ml; el 100% de cepas de Enterobacter con 0.5 mcg/ml, y el 100% de cepas Klebsiella fueron inhibidas por 0.5 mcg/ml; todos estos BGN corresponden al 96.5% del total de cepas estudiadas

Tratamento da infecçäo urinária aguda em crianças com ácido pipemídico / Treatment of acute urinary infection in children with pipemidic acid

Medicaram-se 30 crianças de ambos os sexos (20 masculinas e 10 femininas), com idade variável entre oito e 14 anos, com infecçäo urinária aguda näo recidivante e procedentes da Capital (Recife). Todas elas encontravam-se em regime de internamento em várias unidades hospitalares do município de Recife. Administramos o ácido pipemídico na dose de 400mg/dia, fracionada em duas tomadas, com intervalo de 12 horas, até a remissäo dos sintomas (5 a 10 dia). Foram efetuados exames de laboratório antes, durante e no 15§ dia após o tratamento, constando das seguintes provas: hemograma completo, tempo de protrombina, transaminases, bilirrubinas, uréia, creatinina, urinálise e urocultura com antibiograma. Verificamos cura clínica e microbiológica em 83,3% dos casos, ou seja, 25 pacientes e insucessos em 16,6%, ou cinco doentes. As manifestaçöes colaterais observadas foram vômitos e epigastralgia, de intensidade moderada, em duas crianças. Conclui-se que o quimioterápico em tela é eficaz e bem tolerado no tratamento da infecçäo urinária aguda em crianças

Computed tomographic evaluation of pulmonary mass lesions in chest radiograph

Until recently, soliatry coin lesions of pulmonary disease hs been a conspicious problem in radiologic diagnosis, It is now well informed that CT has offered high resolution with its objective CT numbers to porvide additional information in terms of anatomic changes. Here by the aid of CT, the author gas reviewed retrospectively patients with various shape of round masses thus illustrating the advantage of it over conventional X-ray in diagnosis. 1. Total 53 patients, including 34 males and 19 females, aging between 19 to 76years old with nodule or mass of any size ranging 1 to 13cm in diameter were observed. 2. On palin chest X-raythey were indentified where 50 patients has single round nodule or mass, only one had two masses which were ecchinococcal cysts, and the rest two had invisible lesions only detected by CT. 3. With philips tomoscan 310, CTscan was taken with 12mm thick slice during quiet respiration. Using the ROI cursor the average CT number of thecentral area was calculated 1.0cm in side the outer border of the mass. 4. As a consequence of their pathologic features, they were itemized to 4 group as 36 solid, 9 cystic, 4 consolidative and 4 cavitary lesions. 5. Correctdiagnosis of 3 cystic lesions, 4 diffuse calcification, 1 A-V malformation were available by CT densitometry. 6.By the aid of better resolution and additional cross-sectional orientation of CT, 3 extrapulmonary lesions, 3segmental consolidations, 2 bronchocele, and 2 solitary metastasis, were helpful in diagnosis. 7. Also helpful indetermining the extent of intrathoracic extent of bronchogenic carcinoma for the same reason but given clues werenot more than the ordinary. 8. However, the limitation of the CT densitometry led to miss diagnosis of 3 examplesof cystic vs. solid lesions, and CT density of noncalcified granuloma together with bronchogenic carcinoma, didnot have a clear cut separation in between.

O acido pipemidico no tratamento da infeccao urinaria. / Pipemidic acid in the urinary tract infection treatment

Vinte e quatro pacientes de sexo feminino, acometidas por infeccoes do trato urinario que procuraram o Pronto-Socorro de Ginecologia do Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo, foram tratadas com acido pipemidico, 400 mg 2 vezes ao dia por via oral, por um periodo de sete dias. Do estudo foram excluidas pacientes com molestia imflamatoria pelvica, vulvovaginites ou gestantes.Exame de urina tipo I, cultura e teste de sensibilidade bacteriana foram realizados antes e 10 dias apos o tratamento. O agente mais frequente encontrado foi o E. coli seguido por Staphylococcus saprophyticus, alem de Proteus mirabilis e Citrobacter sp O acido pipemidico mostrou-se altamente eficaz, proporcionando cura bacteriologica e desaparecimento ou melhora da sintomatologia em 87,5% dos casos. A tolerabilidade do medicamento foi muito boa, tendo sido observado apenas um caso de desconforto abdominal e um outro de sensacao de plenitude epigastrica com prurido cutaneo generalizado, anbos transitorios e de leve intensidade