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1.
Braz. j. microbiol ; 48(1): 1-2, Jan.-Mar. 2017.
Article Dans Anglais | LILACS | ID: biblio-839362

Résumé

Abstract Pediococcus acidilactici strain K3 is an alcohol-tolerant lactic acid bacterium isolated from nuruk, which is a traditional Korean fermentation starter for makgeolli brewing. Draft genome of this strain was approximately 1,991,399 bp (G+C content, 42.1%) with 1525 protein-coding sequences (CDS), of which 44% were assigned to recognized functional genes. This draft genome sequence data of the strain K3 will provide insights into the genetic basis of its alcohol-tolerance.


Sujets)
Adaptation biologique/effets des médicaments et des substances chimiques , Adaptation biologique/génétique , Génome bactérien , Éthanol/pharmacologie , Pediococcus acidilactici/effets des médicaments et des substances chimiques , Pediococcus acidilactici/génétique , Acide lactique/biosynthèse , Biologie informatique/méthodes , Génomique/méthodes , Éthanol/métabolisme , Fermentation , Séquençage nucléotidique à haut débit , Annotation de séquence moléculaire , Pediococcus acidilactici/isolement et purification , Pediococcus acidilactici/métabolisme
2.
Braz. j. microbiol ; 46(4): 1009-1018, Oct.-Dec. 2015. tab, graf
Article Dans Anglais | LILACS | ID: lil-769674

Résumé

Abstract Recently, there has been a lot of interest in the utilization of rhodococci in the bioremediation of petroleum contaminated environments. This study investigates the response of Rhodococcus erythropolis IBBPo1 cells to 1% organic solvents (alkanes, aromatics). A combination of microbiology, biochemical, and molecular approaches were used to examine cell adaptation mechanisms likely to be pursued by this strain after 1% organic solvent exposure. R. erythropolis IBBPo1 was found to utilize 1% alkanes (cyclohexane, n-hexane, n-decane) and aromatics (toluene, styrene, ethylbenzene) as the sole carbon source. Modifications in cell viability, cell morphology, membrane permeability, lipid profile, carotenoid pigments profile and 16S rRNA gene were revealed in R. erythropolis IBBPo1 cells grown 1 and 24 h on minimal medium in the presence of 1% alkanes (cyclohexane, n-hexane, n-decane) and aromatics (toluene, styrene, ethylbenzene). Due to its environmental origin and its metabolic potential, R. erythropolis IBBPo1 is an excellent candidate for the bioremediation of soils contaminated with crude oils and other toxic compounds. Moreover, the carotenoid pigments produced by this nonpathogenic Gram-positive bacterium have a variety of other potential applications.


Sujets)
Adaptation biologique/effets des médicaments et des substances chimiques , Adaptation biologique/génétique , Adaptation biologique/physiologie , Adaptation biologique/toxicité , Composés chimiques organiques/effets des médicaments et des substances chimiques , Composés chimiques organiques/génétique , Composés chimiques organiques/physiologie , Composés chimiques organiques/toxicité , /effets des médicaments et des substances chimiques , /génétique , /physiologie , /toxicité , Rhodococcus/effets des médicaments et des substances chimiques , Rhodococcus/génétique , Rhodococcus/physiologie , Rhodococcus/toxicité , Solvants/effets des médicaments et des substances chimiques , Solvants/génétique , Solvants/physiologie , Solvants/toxicité
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