Preformulation studies for the development of a microemulsion formulation from Ambrosia peruviana All, with anti-inflammatory effect

Abstract Natural products are considered an important source of the therapeutic arsenal currently available. Among these alternatives are the seeds of Ambrosia peruviana (altamisa), whose extract has shown an anti-inflammatory effect. The main objective of this work was to perform a preformulation study of Ambrosia peruviana seeds ethanolic extract, where the main factors that affect the physical, chemical, and pharmacological stability of the extract were evaluated, as well as a compatibility study by differential scanning calorimetry (DSC) analysis against different excipients. A dry extract was obtained by rotary evaporation of the seeds macerated with 96% ethanol. The anti-inflammatory activity was determined by measuring its effect on NO production in RAW 264.7 macrophages, stimulated with LPS. The results showed that the dry extract maintained its stability over time when stored at a temperature of 4 and 25ºC, demonstrating its biological activity, the content of phenolic compounds, and its physicochemical parameters remain practically invariable. However, when exposed to high temperatures (60 ºC) it was affected. The thermal analysis revelated that the behavior of most of the selected excipients and the dry extract was maintained, which indicates that it did not present incompatibilities, therefore they can be candidates for formulating a microemulsion.

effects of Ambrosia maritime L. [damsissa] on some biochemical and histological parameters of diabetic albino rats

Diabetes mellitus is one of the common and widely distributed metabolic diseases all over the world. This disease is characterized by hyperglycemia that results from defects in insulin secretion, insulin action or both. Different medicinal plant species are used as a traditional treatment for diabetes mellitus e.g. Ambrosia maritima, L. [Damsissa] which is one of these plants that its extract was used to treat diabetic patients long times ago. This work was aimed to investigate the antidiabetic, hypolipidemic and antioxidant effects of the aqueous extract of Ambrosia maritima, L. [Damsissa] on the alloxan-induced diabetic male albino rats. This study was performed on thirty male albino rats with an average 100-110 g body weight. The animals were divided into three groups [10 /cage]; Group I [Control untreated-group], Group II [Alloxan-induced diabetic group] and Group III [diabetic group treated orally with "28.5 mg/ kg body wt. twice/ day" of the plant extract]. The biochemical results showed marked decline [p<0.01] in the levels of the serum insulin, body weight, total proteins, albumin, globulin and HDL accompanied with marked elevation [p<0.001] in the levels of fasting blood glucose, levels of HOMA_IR, AST, ALT, GGT, urea, creatinine, uric acid, serum TC, TG, LDL, VLDL and ratios of TC/HDL and LDL/HDL [risk factors] in diabetic rats in comparison with the control group. Daily management of the diabetic rates with aqueous extract of Damsissa showed significant improvement in most of these parameters. Histologically, considerable improvement in the morphological changes that was observed in diabetic groups had been detected after treatment with Damsissa in liver, kidney and pancreatic tissues in comparison to the control group. It could be concluded that Ambrosia maritima, L. [Damsissa] can be used as an antidiabetic drug that can lower blood glucose concentration and guard against the negative effects of diabetes