Résumé
Cystic echinococcosis (CE) is an anthropozoonotic disease with worldwide distribution and is caused by the cestode Echinococcus granulosus. Anaphylactic shock induced by CE rupture is a serious complication especially in patients with hydatid infections, as the resulting leakage of fluid contains highly toxic endogenous antigen. We aimed to isolate and identify the antigens of specific IgE and IgG1 (sIgE and sIgG1) in E. granulosus cyst fluid (EgCF). Crude antigen for EgCF was prepared from E. granulosus-infected sheep liver. Antigens were separated and identified by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (1D SDS-PAGE), two-dimensional gel electrophoresis (2-DE), and immunoblotting. Results of 1D SDS-PAGE and immunoblotting showed that 40.5 kDa protein was the major antigen of sIgE, and 35.5 kDa protein was the major antigen of sIgG1 in EgCF. Results of 2-DE and immunoblotting showed that main antigens of sIgE in EgCF were four proteins with pI values ranging from 6.5 to 9.0 and a molecular weight of 40.5 kDa. Main antigens of sIgG1 in EgCF were five proteins with pI values ranging from 6.5 to 9.0 and a molecular weight of 35.5 kDa. The antigens identified for sIgE and sIgG1 can provide critical insights into cellular and molecular mechanisms underlying anaphylactic shock induced by CE.
Sujets)
Humains , Animaux , Mâle , Femelle , Enfant , Adolescent , Adulte , Jeune adulte , Immunoglobuline E/sang , Immunoglobuline G/sang , Echinococcus granulosus/immunologie , Échinococcose/complications , Anaphylaxie/parasitologie , Antigènes d'helminthe/immunologie , Test ELISA , Immunotransfert , Études cas-témoins , Échinococcose/immunologie , Électrophorèse sur gel de polyacrylamide , Anaphylaxie/immunologie , Antigènes d'helminthe/sangRésumé
La enfermedad hidatidica (Equinococosis) existe en todo el mundo: Su frecuencia en el ser humano depende de la incidencia de la enfermedad en huéspedes intermediarios. El quiste hidatidico más frecuente es el causado por el Echinococcus Granulosus, estos pueden alojarse en cualquier órgano o tejido pero la mayoría de las veces se aloja en el hígado y pulmones. El goteo del líquido del quiste produce sensibilización y origina eosinofilia; si el goteo es importante, ya sea espontáneo, traumático o por intervención quirúrgica, puede presentar una reacción anafilactica grave o hasta mortal. Presentamos un caso de reacción anafilactica en un paciente con quiste hidatidico el cual fue tratado quirúrgicamente. Se presenta la revisión de la literatura y se sugiere un manejo transoperatorio