Clinical and Biochemical Profiles according to Homeostasis Model Assessment-insulin Resistance (HOMA-IR) in Korean Women with Polycystic Ovary Syndrome

OBJECTIVES: The aim of this study was to investigate the clinical and biochemical profiles according to homeostasis model assessment of insulin resistance (HOMA-IR) in Korean polycystic ovary syndrome (PCOS) patients. METHODS: In 458 PCOS patients diagnosed by the Rotterdam European Society for Human Reproduction and Embryology (ESHRE) criteria, measurements of somatometry, blood test of hormones, glucose metabolic and lipid profiles, and transvaginal or transrectal ultrasonogram were carried out. HOMA-IR was then calculated and compared with the clinical and biochemical profiles related to PCOS. The patients were divided into 4 groups by quartiles of HOMA-IR. RESULTS: The mean level of HOMA-IR was 2.18 +/- 1.73. Among the four groups separated according to HOMA-IR, body weight, body mass index (BMI), waist-to-hip ratio (WHR), triglyceride (TG), total cholesterol, high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, lipid accumulation product (LAP) index, high-sensitivity C-reactive protein (hs-CRP), Apoprotein B, free testosterone, and sex hormone binding globulin (SHBG) were found to be significantly different. TG, LAP index, glucose metabolic profiles, and hs-CRP were positively correlated with HOMA-IR after adjustment for BMI. CONCLUSION: Our results suggest that the clinical and biochemical profiles which are applicable as cardiovascular risk factors are highly correlated with HOMA-IR in Korean women with PCOS.

Optimization of the preparation process for fusion protein Fv-LDP that composes lidamycin apoprotein and single-chain Fv antibody directed against type IV collagenase / 药学学报

This study is to optimize the preparation process of fusion protein Fv-LDP which was expressed in the form of inclusion body and consisted of lidamycin apoprotein LDP and single-chain Fv antibody (scFv) directed against type IV collagenase. The preparation and the dissolution of inclusion body, the immobilized metal affinity chromatography of the target protein and the renaturization by stepwise dialysis were optimized by single-factor analysis or orthogonal design. In addition, the refolded fusion protein Fv-LDP was refined by Sephadex G-75 chromatography followed by fluorescence-activated cell sorter (FACS)-based saturation binding assay to measure its antigen-binding activity. After optimization of the process, the purity of fusion protein Fv-LDP existed in the inclusion body was 63.9% and the corresponding solubility was 95.7%; Under denaturing conditions, the purity of fusion protein Fv-LDP was more than 95% after the purification process. The percentage of monomeric fusion protein Fv-LDP was 60% after the refolding process, while it was further refined to 85% which was 5.6-fold higher than that of the initial refolding condition. The refined fusion protein Fv-LDP could bind to human lung adenocarcinoma PAa cells and human hepatoma BEL-7402 cells with the dissociation constants (Kd) of 0.176 micromol x L(-1) and 0.904 micromol x L(-1), respectively. The preparation process of fusion protein Fv-LDP has been successfully optimized, which provides the experimental basis for the production and future development of fusion protein Fv-LDP, and might serve as a relatively practical system for the preparation of other scFv-based proteins expressed in the form of inclusion body.

Relationship between Serum Cystatin C and Arterial Stiffness in Type 2 Diabetes Patients with Normal Renal Function

OBJECTIVE: Several studies showed that increased arterial stiffness is an independent risk factor for cardiovascular disease. Pulse wave velocity (PWV) is known as a marker for large vessel stiffness. Recent studies show that serum cystatin C is associated with PWV and may predict future cardiovascular events, even in subjects with normal renal function. However, there have been few studies for the relationship between cystatin C and arterial stiffness in patients with type 2 diabetes (T2DM). In this study, we investigated the relationship between serum cystatin C and branchial-ankle PWV in T2DM patients with normal renal function. METHODS: Patients with urinary albumin/creatinine ratio (ACR) higher than 300 microg albumin/mg creatinine, estimated glomerular filtration rate (eGFR) less than 60 mL/min were excluded. A total of 88 patients (47 male/41 female; age, 59+/-2 years; ACR, 33+/-5 microg/mg) were included. Doppler-derived aortic PWV and serum cystatin C were measured. RESULTS: Cystatin C is significantly related to age (r=0.51, P<0.001), hemoglobin A1c (r=-0.23, P<0.05), high density lipoprotein-cholesterol (r=-0.22, P<0.05), apoprotein A (r=-0.22, P<0.05), and eGFR (r=-0.56, P<0.001). Aortic PWV is significantly associated with age (r=0.29, P<0.01), cystatin C (r=0.33, P<0.005), and eGFR (r=-0.24, P<0.05). In multiple regression analysis, there is significant association between aortic PWV and serum cystatin C levels. CONCLUSION: Serum cystatin C is significantly associated with arterial stiffness in T2DM patients with normal renal function. Our results suggest that cystatin C could be a marker for early atherosclerosis in T2DM patients.

Changes in serum lipids in patients with colorectal cancer

To evaluate and assess disruptions of serum lipids at patients having a colorectal cancer. Our prospective study interested 30 patients, from 26 to 93 year old, presenting a colorectal cancer confirmed histologically, examined during the period going from March 2003 to April 2004. Thirty healthy controls were examined in parallel. All patients undergo three blood samples respectively in preoperative, 48h and 6 months after surgical operation. The analyses carried out were determination of a total serum cholesterol, HDL [high density lipoprotein] and LDL [low density lipoprotein] cholesterol, serum triglyceride and serum apoprotein [AI and B]. We noticed a decrease of total serum cholesterol level in 43% of the cases associated to the reduction of the HDL and the LDL cholesterol in respectively 30% and 76% of cases. The mean values of total serum cholesterol, HDL and LDL cholesterol rates were significantly lower for patients compared to those of controls [p respectively: 0.001; 0.04 and 0.001]. Moreover, the level of total serum cholesterol varied significantly with tumor localization [p= 0, 02]. Serum lipid disruptions affect essentially total cholesterol, HDL and LDL cholesterol. It would be therefore interesting to evaluate their rate at the basal state in order to follow their evolution after treatment in colorectal cancer

Optimization of the assembly efficiency for lidamycin chromophore bound to its apoprotein: a case study using orthogonal array / 生物医学与环境科学（英文）

<p><b>OBJECTIVE</b>Lidamycin (LDM) can be dissociated to an apoprotein (LDP) and an active enediyne chromophore (AE). The detached AE can reassemble with its LDP-containing fusion protein to endow the latter with potent antitumor activity. However, the reassembly of AE with LDP is affected by several factors. Our aim was to optimize the assembly efficiency of the AE with a LDP-containing fusion protein and investigate the influence of several factors on the assembly efficacy.</p><p><b>METHODS</b>A method based on RP-HPLC was developed to analyze the assembly rate, and an orthogonal experimental design L(9) (3(4)) was used to investigate the effects of temperature, assembly time, pH and molecular ratio of LDP-containing fusion protein to AE on the assembly rate. Furthermore, the determined optimum conditions for the assembly rate of the LDP-containing fusion protein with AE were applied and evaluated.</p><p><b>RESULTS</b>A calibration curve based on the LDM micromolar concentration against the peak-area of AE by HPLC was obtained. The order in which individual factors in the orthogonal experiment affected the assembly rate were temperature>time>pH>molar ratio of AE to protein and all were statistically significant (P<0.01). The optimal assembly conditions were temperature at 10°C, time of 12 h, pH 7.0, and the molar ratio of AE: protein of 5:1. The assembly rate of AE with a LDP-containing fusion protein was improved by 23% after condition optimization.</p><p><b>CONCLUSION</b>The assembly rate of chromophore of lidamycin with its LDP-containing fusion protein was improved after condition optimization by orthogonal design, and the optimal conditions described herein should prove useful for the development of this type of LDP-containing fusion protein.</p>

Binding capability of lidamycin apoprotein to human breast cancer detected by tissue microarrays / 药学学报

This study is to investigate the binding capability of lidamycin apoprotein (LDP), an enediyne-associated apoprotein of the chromoprotein antitumor antibiotic family, to human breast cancer and normal tissues, the correlation of LDP binding capability to human breast cancer tissues and the expression of tumor therapeutic targets such as VEGF and HER2. In this study, the binding capability of LDP to human breast cancer tissues was detected with tissue microarray. The correlation study of LDP binding capability to human breast tumor tissues and relevant therapeutic targets was performed on breast cancer tissue microarrays. Immunocytochemical examination was used to detect the binding capability of LDP to human breast carcinoma MCF-7 cells. As a result, tissue microarray showed that LDP staining of 73.2% (30/41) of breast cancer tissues was positive, whereas that of 48.3% (15/31) of the adjacent normal breast specimens was positive. The difference between the tumor and normal samples was significant (Chi2 = 4.63, P < 0.05). LDP immunoreactivity in breast cancer correlated significantly with the overexpression of VEGF and HER2 (P < 0.001 and < 0.01, r = 0.389 and 0.287, respectively). Determined with confocal immunofluorescent analysis, LDP showed the binding capability to mammary carcinoma MCF-7 cells. It is demonstrated that LDP can bind to human breast cancer tissues and there is significant difference between the breast cancer tissues and the corresponding normal tissues. Notably, the binding reactivity shows positive correlation with the expression of VEGF and HER2 in breast carcinoma tissues. The results imply that LDP may have a potential use as targeting drug carrier in the research and development of new anticancer therapeutics. This study may provide reference for drug combination of LDM and other therapeutic agents.

Effects of L-carnitine supplementation on serum lipids and apoproteins in hemodialysis patients with LP[a] hyperlipoproteinemia

Lipid abnormalities, especially high serum Lp[a] concentrations, are one of the major causes of cardiovascular diseases in hemodialysis patients. The present study was designed to investigate the effects of L-carnitine supplementation on serum lipids and apoproteins in hemodialysis patients with Lp[a] hyperlipoproteinemia. The study was a randomized clinical trial in which 36 hyper Lp[a] hemodialysis patients [23 males and 13 females] with serum Lp[a] more than 30 mg/dl were randomly assigned to receive either a daily oral carnitine supplement of 1000mg [carnitine group] or no supplement [control group] for 12 weeks. At the baseline and the end of the period 5ml blood were collected after a 12 to 14-hour fast from each patient before dialysis and serum free carnitine, triglyceride, total cholesterol, HDL-C, LDL-C, apoAI, apoB100, Lp[a], IL-6 and albumin were measured. As compared to the initial values, the mean serum free carnitine concentration increased significantly in the carnitine group at the end of the period [P<0.001], while serum triglyceride [P<0.05], total cholesterol [P<0.001] and IL-6 [P<0.001] decreased significantly. No significant changes were observed in the serum concentrations of free carnitine, triglyceride, total cholesterol and IL-6 in the control group. In addition, there were no significant differences between the 2groups as regards mean changes of the serum HDL-C, LDL-C, apoAI, apoB100, Lp[a], and albumin levels. The results of the present study indicate that an L-carnitine supplement has no effect on serum Lp[a] concentration in hemodialysis patients with Lp[a] hyperlipoproteinemia, but it may be effective in preventing cardiovascular diseases by reducing serum triglyceride and total cholesterol concentrations in these patients

[Effects of soy consumption on serum lipid and apoprotein levels in peritoneal dialysis patients]

Lipid abnormalities, especially high serum Lp[a] concentration, is one of the major causes of cardiovascular diseases in peritoneal dialysis patients. The present study was designed to investigate the effects of soy consumption on serum lipid and apoprotein levels in peritoneal dialysis patients. The study was a randomized clinical trial in which 40 peritoneal dialysis patients [20 males and 20 females] were randomly assigned to either a soy or a control diet. The patients in the soy group received 28 g/d textured soy flour [containing 14 g soy protein] for 12 weeks, while the patients in the control group consumed their usual diet without any soy. At the baseline and at the end of the period, from each patient 5 ml blood were collected after a 12- to 14-hour fast and serum triglyceride, total cholesterol, HDL-C, LDL-C, apoAI, apoB100, Lp[a], TNF-alpha, albumin, and phosphorus measured. The serum Lp[a] concentration in more than 86% of the peritoneal dialysis patients was above the normal range. As compared to the baseline value, the mean serum Lp[a] concentration decreased significantly by 41% [P<0.01] in the soy group at the end of 8-week period, and the reduction was significant as compared to the control group [P<0.05]. The mean serum Lp[a] concentration did not change significantly in the control group. There were no significant differences between the 2 groups with regard to mean changes in the serum triglyceride, total cholesterol, HDL-C, LDL-C, apoB100, apoAI, TNF-alpha, albumin or phosphorus levels. The results of the present study indicate that soy consumption reduces serum Lp[a] concentration considerably in peritoneal dialysis patients. Therefore, it may be effective in preventing cardiovascular diseases in these patients

[Effects of combination of vitamin E and nicotinic acid on serum lipids and apoproteins of hypertriglyceridemic hemodialyzed patients]

Oxidative stress and lipid abnormalities are two major risk factors for development of atherosclerosis among hemodialyzed patients. Administrating of Lipidnormalising agents, solely or in combination together, can not correct all lipid abnormalities in hemodialyzed patients. The present study, therefore, was desinged to evaluate the effects of combination therapy of vitamin E and tolerable doses of nicotinic acid on serum lipids and apoproteins in hypertriglyceridemic hemodialyzed patients. The study was a double-blind randomized clinical trial. Thirty-nine hemodialyzed patients with fasting triglyceride range between 230 and 500 mg/dl were randomly assigned into three groups, receiving combination of vitamin E [600mg/d] and nicotinic acid [500mg/d], nicotinic acid alone [500mg/d], and placebo, respectively. All patients received their supplements for 13 weeks. The blood samples were collected after a 12 to 14-hour duration of fasting at the beginning of the study, followed by other samplings performed at the end of sixth and thirteenth weeks, respectively, and serum lipids and apoproteins were measured. accordingly. During the study, the mean serum triglyceride level was significantly reduced in the group receiving combination therapy of vitamin E and nicotinic acid, compared to the placebo group. Compared to that of placebo group, mean serum HDL-C levels were significantly increased two groups of combination therapy, and nicotinic acid alone, although LDL-C/HDL-C ratios were significantly decreased. There was no significant difference in the means of total cholesterol of serum, LDL-C, apoAI, apoB100 and Lp[a] between three groups. It is concluded that combination therapy of vitamin E and nicotinic acid in hypertriglyceridemic hemodialyzed patients can result in improvement in almost every lipid abnormalities, but except high levels of Lp[a]

Vesicular transport of Fe and interaction with other metal ions in polarized Caco2 Cell monolayers

Two aspects of the mechanisms by which iron is absorbed by the intestine were studied in the Caco2 cell model, using 59Fe(II)-ascorbate. Data showing the importance of vesicular processes and cycling of apotransferrin (apoTf) to uptake and overall transport of Caco2 cell monolayers (or basolateral 59Fe release) were obtained by comparing effects of: a) adding apoTf to the basal chamber; b) adding vesicular transport inhibitors; or c) cooling to 4°C. These showed that apoTf may be involved in as much as half of Fe transfer across the basolateral membrane, and that vesicular processes may also play a role in non-apoTf-dependent Fe transport. Studies were initiated to examine potential interactions of other metal ions with Fe(II) via DMT1. Kinetic data showed a single, saturable process for uptake of Fe(II) that was pH dependent and had a Km of 7 ìM. An excess of Mn(II) and Cu(I) over Fe(II) of 200: 1 (ìM: ìM) in 1 mM ascorbate markedly inhibited Fe uptake. The kinetics were not competitive. Km increased and Vmax decreased. We conclude that vesicular transport, involving endo- and exocytosis at both ends of the enterocyte, is a fundamental aspect of intestinal iron absorption and that DMT1 may function as a transporter not just for divalent but also for monovalent metal ions.

Inhibitory effect of deferoxamine on Paracoccidioides brasiliensis survival in human monocytes: reversal by holotransferrin not by apotransferrin

Os mecanismos utilizados pelo Paracoccidioides brasiliensis para sobreviver em células fagocitárias ainda não estão elucidados. O metabolismo celular férrico é muito importante para o crescimento de inúmeros patógenos intracelulares cuja capacidade de se multiplicarem em fagócitos mononucleares é dependente da disponibilidade intracelular do íon ferro. Assim, o objetivo deste trabalho foi investigar o papel do ferro intracelular sobre a capacidade do P. brasiliensis sobreviver em monócitos humanos. O tratamento de monócitos com deferoxamina, uma droga quelante, diminuiu a sobrevivência de leveduras do fungo de forma dose-dependente. O efeito inibidor da deferoxamina sobre a sobrevivência do P. brasiliensis foi revertido por transferrina saturada com ferro (holotransferrina) mas não por transferrina insaturada (apotransferrina). Estes resultados sugerem que a sobrevivência do P. brasiliensis em monócitos humanos é dependente do íon ferro.

Niveles de apoproteínas B, A1 y CIII como marcadores de riesgo cardiovascular en adolescentes delgados y obesos / Levels of apoproteins B, A1 and CIII as markers for cardiovascular risk in lean and obese adolescents

Las enfermedades cardiovasculares constituyen un problema de salud pública de la población adulta. Debido a que las alteraciones ateroscleróticas tienen su origen en la niñez, se hace necesario identificar marcadores bioquímicos en etapas tempranas de la vida, como en la adolescencia. Se estudiaron 79 adolescentes (48 hembras y 31 varones) en edades comprendidas entre 13 y 17 años. A cada adolescente se le realizó una historia clínica. Se evaluó el estado puberal de acuerdo a los criterios de Tanner. Además se realizó la evaluación antropométrica a través de peso, talla, índice de masa corporal (IMC) y medición de cintura, cadera y los pliegues subcutáneos. Se calcularon los índices de centralidad (PSE/PT) y de obesidad (PSE + PB + PT). Después de un ayuno de 12 horas, se determinaron en suero los niveles basales de glicemia, colesterol total (CT), triglicéridos (TG), colesterol de las lipoproteínas de baja densidad (LDL-C) y alta densidad (HDL-C) por métodos enzimáticos y los niveles de insulina basal por radioinmunoensayo (RIA). Se determinaron las concentraciones de apoproteínas A1, B y CIII por inmunoturbimetría. De acuerdo a su IMC y tomando como punto de corte 25 kg/m2 se observó que el 35 por ciento de las hembras y el 16 por ciento de los varones eran obesos. Al mismo tiempo, el 85 por ciento de las hembras y el 58 por ciento de los varones presentaron hiperinsulinemia (insulina basal >12µU/mL). Las medidas de circunferencia, pliegues e índices de centralidad y obesidad fueron superiores (p<0,05) en los varones comparados con las hembras. La media general de insulina tanto para hembras como para varones resultó más alta de acuerdo al punto de corte establecido para insulina en nuestro laboratorio, pero fue significativamente superior (p<0,05) en las hembras. Esto se acompañó de niveles más altos de CT en las hembras y niveles más bajos de HDL-C en los varones. La apo A1 se encontró asociada en forma negativa con el índice de obesidad y positivamente con la HDL-C. La apo B se relacionó con los niveles de CT y LDL-C, mientras que la apo CIII se asoció positivamente con las concentraciones basales de insulina, TG y VLDL-C. Estos resultados sugieren que la apo CIII pareciera ser un buen marcador de niveles elevados de insulina, insulino resistencia y de riesgo cardiovascular precoz.e glicemia, colesterol total (CT)

Coexistence of Cerebral Amyloid Angiopathy and an Arteriovenous Malformation in a Young Adult

The prevalence and cerebral hemorrhage of cerebral amyloid angiopathy(CAA) are age-related. It is rare in young adults. The authors report on CAA coexisting with an arteriovenous malformation(AVM) in a 30-year-old male, who present with the sudden onset of headache and vomiting. Magnetic resonance imaging revealed a cerebral hemorrhage with an AVM. The AVM was completely removed through the hematoma and the histological section obtained from the periphery of the hematoma showed the typical findings of CAA. The epsilon4 allele of apoprotein E(apoE) was identified in genotype determination.

Catalysis of lyase-isomerase PecE/PecF for several apophycobiliproteins / 生物工程学报

Phycoerythrocyanin(PEC) lyase-isomerase PecE/PecF from Mastigocladus laminosus is the specific enzyme for biosynthesis of PEC alpha-subunit(alpha-PEC). In this work, the specificity of PecE/PecF on substrate apoproteins was reported. PecE/PecF could catalyse the reconstitution of phycocyanobilin(PCB) with apoproteins of alpha-PEC from two different subspecies of Mastigocladus laminosus, as well the site-directed mutated apoprotein of alpha-PEC with Trp at 128 to Phe in vitro, but could not catalyse the reconstitution of PCB with apoprotein of phycocyanin alpha-subunit(alpha-CPC) from Mastigocladus laminosus. The surfactant Triton X-100 had no effect for the reconstitution of alpha-PEC, while it could improve the reconstitution of PCB with apoprotein of alpha-CPC.

Methylenetetrahydrofolate reductase polymorphism, homocysteine, lipid levels and susceptibility to preeclampsia in Korean women / 대한산부인과학회잡지

OBJECTIVE: The purpose was to test the hypothesis that the common missense mutation of 5,10-methylenetetrahydrofolate reductase(MTHFR) is more prevalent among preeclamptic women compared with control and also was to determine whether homocysteine and other lipid profile is changed in pregnant women with preeclampsia. METHODS: We measured plasma homocysteine, cholesterol, HDL, LDL, triglyceride, apoprotein B, vitamin B12, and folate in 48 pregnant women without preeclampsia and 22 women with preeclampsia. And the MTHFR genotype was determined with a polymerase chain reaction/restriction fragment length polymorphism method. Results were analyzed with a X2 contingency table and T-test. RESULTS: The prevalence of the MTHFR C677T mutation was not significantly different between the population studied. There was no significant difference in the level of plasma homocysteine, cholesterol, HDL, LDL, triglyceride, apoprotein B, and folate between controls and preeclamptic women. Furthermore, there was a significant difference in the level of plasma vitamin B12 between the population studied. CONCLUSION: These data suggest that the MTHFR C677T mutation is not a risk factor for preeclampsia in this population. Plasma homocysteine, cholesterol, HDL, LDL, triglyceride, apoprotein B, and folate level are not elevated in preeclamptic women. However, the plasma vitamin B12 level is elevated in preeclamptic women. Further studies are necessary to determine why the plasma vitamin B12 level is elevated in preelamptic women although they did not have vitamin drug.

Methylenetetrahydrofolate reductase polymorphism, homocysteine, lipid levels and susceptibility to preeclampsia in Korean women / 대한산부인과학회잡지

OBJECTIVE: The purpose was to test the hypothesis that the common missense mutation of 5,10-methylenetetrahydrofolate reductase(MTHFR) is more prevalent among preeclamptic women compared with control and also was to determine whether homocysteine and other lipid profile is changed in pregnant women with preeclampsia. METHODS: We measured plasma homocysteine, cholesterol, HDL, LDL, triglyceride, apoprotein B, vitamin B12, and folate in 48 pregnant women without preeclampsia and 22 women with preeclampsia. And the MTHFR genotype was determined with a polymerase chain reaction/restriction fragment length polymorphism method. Results were analyzed with a X2 contingency table and T-test. RESULTS: The prevalence of the MTHFR C677T mutation was not significantly different between the population studied. There was no significant difference in the level of plasma homocysteine, cholesterol, HDL, LDL, triglyceride, apoprotein B, and folate between controls and preeclamptic women. Furthermore, there was a significant difference in the level of plasma vitamin B12 between the population studied. CONCLUSION: These data suggest that the MTHFR C677T mutation is not a risk factor for preeclampsia in this population. Plasma homocysteine, cholesterol, HDL, LDL, triglyceride, apoprotein B, and folate level are not elevated in preeclamptic women. However, the plasma vitamin B12 level is elevated in preeclamptic women. Further studies are necessary to determine why the plasma vitamin B12 level is elevated in preelamptic women although they did not have vitamin drug.

Hepatopatía crónica: dislipidemia y enfermedad cardiovascular / Chronic liverdisease: hyperlipidemia and cardiovascular disease

Se midieron las concentraciones de los lípidos y de las apoproteínas apo A1 y apo B100 en 12 pacientes con enfermedad hepática crónica parenquimatosa de diferente severidad y etiología, y en 10 voluntarios sanos. Los resultados se asociaron con variables clínicas y bioquímicas y con resultados de procedimentos utilizados en la evaluación de la enfermedad hapática. Se analizaron las relaciones de los lípidos entre los índices aterogénicos, a fin de determinar el riesgo aterosclerótico en los pacientes. Se observó disminución de Colesterol Total, HDL, apo A1, y apo B100 en el 80 por ciento, 60 por ciento, 50 por ciento, y 70 por ciento de los pacientes respectivamente. Asociaciones importantes fueron: HDL: tiempo de protrombina prolongado y etiología alcohólica; Child-Pugh A y cambios histológicos moderados; apo B100: género femenino, tiempo de protrombina prolongado, bilirrubina elevada, etiología no alcohólica Child Pugh B y cambios histológicos severos. El potencial aterogénico resultó positivo en la relación del colesterol total y HDL (>3.5), y LDL (>1.5), así como la correlación positiva de las apoproteínas entre sí