Actividad de metaloproteinasas de arterias y venas y su regulación mediante doxiciclina / Metalloproteinase activity of arteries and veins and it regulation through doxycicline

Segments of internal mammary arteries and saphenous veins and cultured smooth muscle cells were incubated with and without doxycicline. Metalloproteinases activity was assessed by zymography and Western Blot. Activity of Metalloproteinase-9 in saphenous veins was 217 percent less than in internal mammary arteries. In these vessels doxycicline decreased metalloproteinase-9 activity by 207 percent and metalloproteinase-1 expression. In cultured smooth muscle cells, the median inhibitory concentration of doxycicline for metalloproteinase-2 was 138 uM (r2=0.82). Internal mammary arteries and saphenous veins have different metalloproteinase activities, that are inhibited by doxycicline