Preservation of phenolic compounds on dried leaf infusion of Bauhinia forficata Link

Abstract Bauhinia forficata Link aqueous extract is usually recommended as a phytomedicine to reduce blood glucose levels and its biological activity has been linked to the presence of phenolic compounds from B. forficata preparations. Several drying processes are used in the production of dry herbal extracts, which may influence the chemical composition and efficacy of final herbal medicines. Due to significant chemical changes, defining appropriate drying processes is essential for phytopharmaceutical drug development. In view of this, we analyzed dried B. forficata leaf infusion (BFLI) extracts by HPLC-UV-MSn, followed by molecular networking analysis to evaluate the chemical profiles from dried extracts yielded by freeze-and spray-drying processes. The main metabolites detected included 11 ferulic/isoferulic acid derivatives and 13 glycosylated flavonoids. The qualitative chemical profiles were alike for both drying processes, whereas the relative abundance of some flavonoids was higher using spray-drying. Taken together, our results showed that freeze-and spray-drying preserved the phenolic profile of BFLI and suggested that spray-drying may be the most suitable to obtain its dried products. Along with studying the chemical profiles of dried herbal extracts, evaluating the influence of drying processes on the quality and chemical profiles of final products is pivotal and may benefit future research.

Anticancer activity of Bauhinia racemosa Lam on mice bearing ehrlich ascites carcinoma

PHARMACOLOGICAL studies of the Bauhinia Racemosa Lam which was belongs to the family Caesalpiniaceae revealed that the ethanol extract of leaves of B. racemosa presented analgesic, antipyretic, anti-inflammatory, antispasmodic in the previous research but in the present study the antitumor activity and antioxidant status at different concentration of ethanol extract of Bauhinia racemosa stem bark [40, 80, and 160 micro g/kg] was evaluated against Ehrlich ascites carcinoma [EAC] in mice. The ethanol extract of Bauhinia racemosa stem showed decrease in tumor volume, packed cell volume an viable cell count, and increased the nonviable cell count and mean survival time thereby increasing life span of Ehrlich ascites carcinoma tumor bearing mice. Hematological profile reverted to more or less normal levels in extract treated mice. Treatment with ethanol extract of Bauhinia racemosa decreased the levels of lipid peroxidation and increased the levels of glutathione, superoxide dismutase and catalase. It could be concluded that, the ethanol extract of Bauhinia racemosa stem bark exhibited antitumor effect by modulating lipid peroxidation and augmenting antioxidant defense system in Ehrlich ascites carcinoma bearing mice