Identification of parasite DNA in common bile duct stones by PCR and DNA sequencing

We attempted to identify parasite DNA in the biliary stones of humans via PCR and DNA sequencing. Genomic DNA was isolated from each of 15 common bile duct (CBD) stones and 5 gallbladder (GB) stones. The patients who had the CBD stones suffered from cholangitis, and the patients with GB stones showed acute cholecystitis, respectively. The 28S and 18S rDNA genes were amplified successfully from 3 and/or 1 common bile duct stone samples, and then cloned and sequenced. The 28S and 18S rDNA sequences were highly conserved among isolates. Identity of the obtained 28S D1 rDNA with that of Clonorchis sinensis was higher than 97.6%, and identity of the 18S rDNA with that of other Ascarididae was 97.9%. Almost no intra-specific variations were detected in the 28S and 18S rDNA with the exception of a few nucleotide variations, i.e., substitution and deletion. These findings suggest that C. sinensis and Ascaris lumbricoides may be related with the biliary stone formation and development.

Wandering ascaris coming out through the T-tube tract--a rare occurrence.

A case of ascariasis of the common bile duct in the postoperative period of cholecystectomy and choledocholithotomy with T-tube drainage is reported. The living adult wandering ascaris came out through the T-tube tract immediately after removal of the T-tube. No such previous report has been found in the available literature.

Common bile duct ascariasis

To assess the advantage of palpation of CBD during exploratory laparotomy in patients with acute abdomen. Material and A prospective study was conducted from July 1998 till December 2002 at Tehsil Head Quarter Hospital Matta, District Swat, Saidu Group of Hospitals Swat and DHQ Hospital Timergara District Dir Lower. Eleven patients, 5 male and 6 female [mean age 9.8+2.6 years] were included in this study. All the operations were performed under general anesthesia. Four patients were diagnosed preoperatively by abdominal ultrasonography and subjected to operative extraction of worms on next operation list. Seven patients presented with acute abdomen and turned out to have biliary ascariasis on palpating the CBD during exploratory laparatomy. T tube was inserted in 10 patients to drain the CBD, while in one patient primary repair was done. Their postoperative course was smooth and uneventful. Biliary ascariasis though not a common problem must be considered as a possibility in patients presenting with acute abdomen in endemic areas especially when no other cause for the patient's symptoms is apparent

Biliary Fascioliasis. case report and review of the literature

Hepatobiliary parasitic diseases are rare in Lebanon. We recently encountered biliary fascioliasis in a Lebanese native. The clinical and laboratory findings were nonspecific. The biliary parasite [Fasciola hepatica] was identified by sonography and confirmed at ERCP that has retrieved the parasite from the common bile duct

Ascariasis en vesícula y vías biliares / Ascariasis in gall-bladder and bile ducts

La ascariasis del árbol biliar es una entidad quirúrgica poco común por lo que es susceptible de originar problemas de diagnóstico diferencial. En este trabajo se presenta un caso de colecistitis por áscaris impactado con el conducto cístico y dos casos de áscaris localizados en el colédoco. Se hace un análisis retrospectivo de las características del cuadro clínico que pueden orientar al diagnóstico etiológico. Se concluye que esta entidad debe sospecharse en pacientes de nivel socioeconómico bajo, provenientes del medio rural o de áreas tropicales que presentan síntomas de cólico biliar atípico