Double replicas allow the obtention of longitudinally freeze-fractured Trypanosoma cruzi

The double replica device was used to obtain freeze-fracture replicas of gently pressed cells, allowing the visualization of a large number of longitudinally fractured epimastigote and trypomastigote forms of Trypanosoma cruzi. This technique revealed large areas of the plasma membrane, the region of attachment of the flagellum to the cell body and the branched mitochondria.