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1.
Int. braz. j. urol ; 44(5): 1014-1022, Sept.-Oct. 2018. tab, graf
Article Dans Anglais | LILACS | ID: biblio-975626

Résumé

ABSTRACT Objective: To evaluate the effect of intravesical hyaluronic acid (HA) treatment on inflammatory cells and the severity of inflammation in an interstitial cystitis rat model created with hydrogen chloride (HCL) via immunohistochemical studies and myeloperoxidase activity for the first time in the literature. Materials and Methods: A total of 30 adult female white Rattus Norvegicus rats were divided into 3 groups as the HCL group, hyaluronic acid treatment (HCL-HA) group and control group. Chemical cystitis was created by administering HCL(400 microL,10 mM) except control group. A single dose of intravesical HA(0.5 mL,0.8 mg/mL) was administered to the treatment group. The bladder tissues of all subjects were immunohistochemically stained. The cell surface markers were used to evaluate inflammatory cell infiltration. Mast cell activation and IL-6 was evaluated to assess the inflammation and severity of inflammation, respectively. Myeloperoxidase activity was measured as it shows neutrophil density. Statistical significance was accepted as P<0.05. Results: It was observed that there was rich monocyte, T lymphocyte, B lymphocyte, and Natural Killer cells infiltration and high IL-6 levels in the bladder tissue after the intravesical hydrogen chloride instillation, especially in the stroma layer(p<0.005). In the HCL-HA group, severity of inflammation had statistically significantly regressed to the levels of the control group(p<0.005). An increase was observed in the bladder myeloperoxidase activity of the HCL group compared to the other two groups(p<0.05). Conclusions: Single dose intravesical hyluronic acid instillation reduces inflammatory cell infiltration and the severity of bladder inflammation in the rat model of bladder pain syndrome/interstitial cystitis.


Sujets)
Animaux , Femelle , Rats , Vessie urinaire/effets des médicaments et des substances chimiques , Cystite interstitielle/traitement médicamenteux , Acide hyaluronique/usage thérapeutique , Vessie urinaire/anatomopathologie , Indice de gravité de la maladie , Administration par voie vésicale , Cystite interstitielle/induit chimiquement , Cystite interstitielle/anatomopathologie , Modèles animaux de maladie humaine , Acide chlorhydrique
2.
Int. braz. j. urol ; 40(1): 72-79, Jan-Feb/2014. tab, graf
Article Dans Anglais | LILACS | ID: lil-704176

Résumé

Introduction: Painful bladder syndrome/interstitial cystitis (PBS/IC) pathogenesis is not fully known, but evidence shows that glycosaminoglycans (GAG) of bladder urothelium can participate in its genesis. The loss of these compounds facilitates the contact of urine compounds with deeper portions of bladder wall triggering an inflammatory process. We investigated GAG in urine and tissue of PBS/IC and pure stress urinary incontinence (SUI) patients to better understand its metabolism. Materials and Methods: Tissue and urine of 11 patients with PBS/IC according to NIDDK criteria were compared to 11 SUI patients. Tissue samples were analyzed by histological, immunohistochemistry and immunofluorescence methods. Statistical analysis were performed using t Student test and Anova, considering significant when p < 0.05. Results: PBS/IC patients had lower concentration of GAG in urine when compared to SUI (respectively 0.45 ± 0.11 x 0.62 ± 0.13 mg/mg creatinine, p < 0.05). However, there was no reduction of the content of GAG in the urothelium of both groups. Immunofluorescence showed that PBS/IC patients had a stronger staining of TGF-beta, decorin (a proteoglycan of chondroitin/dermatan sulfate), fibronectin and hyaluronic acid. Conclusion: the results suggest that GAG may be related to the ongoing process of inflammation and remodeling of the dysfunctional urothelium that is present in the PBS/IC. .


Sujets)
Adulte , Sujet âgé , Femelle , Humains , Adulte d'âge moyen , Cystite interstitielle/métabolisme , Glycosaminoglycanes/métabolisme , Incontinence urinaire d'effort/métabolisme , Biopsie , Créatinine/urine , Cystite interstitielle/anatomopathologie , Technique d'immunofluorescence , Glycosaminoglycanes/analyse , Acide hyaluronique/urine , Immunohistochimie , Réaction de polymérisation en chaine en temps réel , Vessie urinaire/anatomopathologie , Incontinence urinaire d'effort/anatomopathologie , Urothélium/métabolisme , Urothélium/anatomopathologie
3.
Int. braz. j. urol ; 36(4): 464-479, July-Aug. 2010. ilus, graf, tab
Article Dans Anglais | LILACS | ID: lil-562113

Résumé

PURPOSE: Interstitial cystitis/painful bladder syndrome (IC/PBS) is characterized by chronic pain, pressure and discomfort felt in the pelvis or bladder. An in-depth shotgun proteomics study was carried out to profile the urinary proteome of women with IC/PBS to identify possible specific proteins and networks associated with IC/PBS. MATERIALS AND METHODS: Urine samples from ten female IC/PBS patients and ten female asymptomatic, healthy control subjects were analyzed in quadruplicate by liquid chromatography-tandem mass spectrometry (LC-MS/MS) on a hybrid linear ion trap-orbitrap mass spectrometer. Gas-phase fractionation (GPF) was used to enhance protein identification. Differences in protein quantity were determined by peptide spectral counting. RESULTS: a-1B-glycoprotein (A1BG) and orosomucoid-1 (ORM1) were detected in all IC/PBS patients, and = 60 percent of these patients had elevated expression of these two proteins compared to control subjects. Transthyretin (TTR) and hemopexin (HPX) were detected in all control individuals, but = 60 percent of the IC/PBS patients had decreased expression levels of these two proteins. Enrichment functional analysis showed cell adhesion and response to stimuli were down-regulated whereas response to inflammation, wounding, and tissue degradation were up-regulated in IC/PBS. Activation of neurophysiological processes in synaptic inhibition, and lack of DNA damage repair may also be key components of IC/PBS. CONCLUSION: There are qualitative and quantitative differences between the urinary proteomes of women with and without IC/PBS. We identified a number of proteins as well as pathways/networks that might contribute to the pathology of IC/PBS or result from perturbations induced by this condition.


Sujets)
Femelle , Humains , Marqueurs biologiques/urine , Cystite interstitielle/étiologie , Protéines/analyse , Protéomique/méthodes , Urine/composition chimique , Maladie chronique , Cystite interstitielle/anatomopathologie , Projets pilotes
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