1.
Experimental & Molecular Medicine
;
: 240-245, 1998.
Article
Dans Anglais
| WPRIM
| ID: wpr-159763
Résumé
We studied the regulation of fibronectin (FN) gene expression by cAMP and phorbol-12-myristate-13-acetate (PMA) in HT-1080 human fibrosarcoma cells. Dibutyryl cAMP increased FN synthesis and mRNA levels, while PMA inhibited the cAMP-induced FN synthesis. In transient transfection assays, cAMP increased FN promoter activity, while PMA paradoxically enhanced the cAMP-induced promoter activity. Stable transfection experiments, however, showed that neither cAMP or PMA alone nor together affected FN promoter activity. These results suggest that PMA antagonizes the cAMP-induced FN gene expression and that both the action of cAMP and the inhibition of its action by PMA may occur at the posttranscriptional level in HT-1080 cells.
Sujets)
Humains , Technique de Northern , Dibutyryl AMP cyclique/pharmacologie , Dibutyryl AMP cyclique/antagonistes et inhibiteurs , Test ELISA , Fibronectines/métabolisme , Fibronectines/génétique , Fibrosarcome/génétique , Régulation de l'expression des gènes , Luciferases/métabolisme , Tests aux précipitines , Régions promotrices (génétique) , ARN messager/métabolisme , 12-Myristate-13-acétate de phorbol/pharmacologie , Transfection , Cellules cancéreuses en culture , beta-Galactosidase/métabolisme
2.
Indian J Cancer
;
1967 Sep; 4(3): 298-303
Article
Dans Anglais
| IMSEAR
| ID: sea-50895