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Experimental & Molecular Medicine ; : 343-352, 2007.
Article Dans Anglais | WPRIM | ID: wpr-201420

Résumé

Asthma was induced by the sensitization and challenge with ovalbumin (OVA) in mice. B-cell activating factor (BAFF) plays a role in mature B cell generation and maintenance. Here, we investigated whether, BAFF expression was changed in OVA-induced mice and whether the control of BAFF expression level alleviates the symptom of bronchial asthma. BAFF expression was detected in alveolar-associated cells surrounding bronchi of OVA-induced mouse lung tissues. BAFF protein was also increased in OVA-induced mouse serum. The increased BAFF transcripts was detected in OVA-induced mouse splenocytes. OVA-induced asthma was associated with the increased number of eosinophils in bronchoalveolar lavage fluid (BALF). When TACI:Fc scavenging soluble BAFF was injected to OVA-induced mice, a significant inhibition was detected in the thickness of airway smooth muscle and glycol-containing cellular elements in airway that were visualized by hematoxylin/eosin Y and periodic acid-Schiff staining, respectively. In addition, when mice were treated with TACI:Fc protein, BAFF protein level was decreased in alveolar-associated cells surrounding bronchi of OVA-induced mouse lung tissues compared to control mice. When compared to OVA-induced control, TACI:Fc treatment reduced the percentage of non-lymphoid cells and no changes were detected in lymphoid cell population. Hypodiploid cell formation in BALF was decreased by OVA-challenge but it was recovered by TACI:Fc treatment. Collectively, data suggest that asthmatic symptom could be alleviated by scavenging BAFF and then BAFF could be a novel target for the develpoment of anti-asthmatic agents.


Sujets)
Animaux , Femelle , Humains , Souris , Apoptose , Asthme/induit chimiquement , Facteur d'activation des lymphocytes B/biosynthèse , Bronches/métabolisme , Liquide de lavage bronchoalvéolaire/cytologie , Granulocytes éosinophiles/anatomopathologie , Fragments Fc des immunoglobulines/génétique , Immunoglobuline G/génétique , Lymphocytes/anatomopathologie , Souris de lignée BALB C , Ovalbumine , Alvéoles pulmonaires/métabolisme , Protéines de fusion recombinantes/génétique , Rate/métabolisme , Protéine TACI/génétique
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