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Asian Pac J Allergy Immunol ; 2001 Mar; 19(1): 49-53
Article Dans Anglais | IMSEAR | ID: sea-37130

Résumé

Human rotavirus is the major etiologic agent of infantile diarrhea on a worldwide scale. In this study, rotaviruses were detected by reverse-transcription PCR in 42 of 83 stool specimens from children below the age of 3 years with acute diarrhea in Bangkok, Thailand, between November 1998 and August 1999. G and P types of all samples were characterized by restriction endonuclease analysis (REA) and multiplex PCR typing assay, respectively. Strain G1P[8] (76.1%) was the predominant type, followed by G1P[6] (2.4%). Strain G1 combined with mixed P[8]/P[6] was identified in 2 specimens (4.8%) and 7 untypeable G strains (16.7%) were observed. This information on the circulating G and P combinations should be useful for understanding the epidemiology of human rotavirus in Bangkok, Thailand.


Sujets)
Maladie aigüe , Séquence nucléotidique , Protection de l'enfance , Enfant d'âge préscolaire , DNA restriction enzymes/analyse , Gastroentérite/classification , Marqueurs génétiques/génétique , Génotype , Humains , Nourrisson , Protection infantile , Nouveau-né , Données de séquences moléculaires , RT-PCR , Infections à rotavirus/classification , Sérotypie , Thaïlande/épidémiologie
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