Immunolocalization of vascular and non vascular expression of VCAM-1 in hyperplastic, dysplastic oral mucosal lesions and squamous cell carcinoma

38 oral biopsy specimens of hyperplastic surface epithelial lesions [8 cases], epithelial dysplastic lesions [12 cases] and squamous cell carcinoma [SCC] [18 cases] as well as six biopsies of normal oral mucosa were processed for immunohistochemical staining with anti VCAM-1 to identify vascular and non vascular expression of VCAM-1 in the hyperplastic and dysplastic oral lesions as well as oral SCC compared to normal oral mucosa and to examine if it has an active role in the pathogenesis of these lesions that could point the way to the developing of new therapeutic stratigies. The results of this study suggested that VCAM-1 expression has essentially a direct relation with the density and distribution of chronic inflammatory cell infiltration. VCAM-1 is not related to the different grades of epithelial dysplasia and tumor cell differentiation. It cann't be also used as an indicator of tumor angiogenesis

Fatores de prognóstico da queilite actínica analisados por imuno-histoquímica e citometria estática: estudo comparativo com leucoplasias associadas a displasias, e carcinomas espinocelulares / Prognostic parameters of actinic cheilitis analysed by immunohistochemical and estatic cytometry: comparative study with leukoplakia combined with dysplasia and squamous cell carcinoma

As lesöes cancerizáveis podem manter-se estáveis, regredir ou sofrer transformaçöes neoplásica. Nos fatores de prognóstico, classicamente estabelecidos, repousam dogmas e elevado grau de subjetividade. Para caracterizar a expressäo imuno-histoquímica de p53, CD31, TGFx e PCNA e avaliar o conteúdo de DNA nuclear pela citometria estática foram selecionados 13 casos de queilite actínicas. Nove casos de hiperplasias, 12 casos de leucoplasias e 13 casos de neoplasias, situados no lábio, foram submetidos aos mesmos exames. Os resultados indicaram as alteraçöes da proteína p53 como as mais significativas nas queilites actínicas. Em lesöes positivas para p53, 50 por cento apresentaram um conteúdo aneuplóide, indicando a precocidade destas alteraçöes e assimilando sua propensäo para malignidade. Nas queilites actínicas, a marcaçäo do TGFx exibiu um padräo intermediário entre lesöes hiperplásicas e neoplásicas. Os índices de proliferaçäo, obtidos pela expressäo de PCNA, foram crescentes a partir das lesöes hiperplásicas até os carcinomas. A densidade vascular, aferida pela contagem de vasos marcados com CD31, apresentou padräo similar. Na análise de citometria de imagem, todas as lesöes hiperplásicas mostraram conteúdo diplóide. As queilites actínicas e leucoplasias, em nove casos, apresentaram histogramas aneuplóides. Os carcinomas exibiram padräo aneuplóide em dez casos. A expressäo dos marcadores p53, CD31, PCNA e TGFx e a mensuraçäo da ploidia, foram caracterizadas em lesöes de queilites actínicas, podendo ser utilizadas como indicadores de prognóstico nas lesöes cancerizáveis

role of immunology in the pathogenesis of inflammatory papillary hyperplasia associated with denture wearers

1 - No significant difference of immunoglobulins level were found between denture and ono denture wearer.2 - Significant increase of immunoglobulins [G and E] level of palatal mucosa with papillary hyperplasia. 3 - In inflammatory papillary hyperplasia, synergistic activity may be present between inflammation and immunity where inflammation may enhance the immune response by maintaining a tissue environment of increased vascularity and permeability