Effect of sodium hypochlorite and calcium hypochlorite on the apical sealing ability of endodontic sealers

Aim: This study aimed to compare the influence of sodium hypochlorite (NaOCl) and calcium hypochlorite [Ca(OCl)2 ] on the apical sealing capacity of AH Plus (Dentsply Maillefer) and Bio-C Sealer (Angelus) endodontic sealers. Methods: Sixty permanent human lower incisors were randomly allocated (http://www.randomized.org), according to the irrigant used, into three groups (n=20): 0.9% sodium chloride (NaCl/Control); 2.5% NaOCl; and 2.5% Ca(OCl)2 . The root canal was prepared with rotary files under 10 mL of the solution corresponding to the experimental group. Each group was subdivided into two (n=10) according to the sealer used for filling: AH Plus (Dentsply Maillefer) or Bio-C Sealer (Angelus). Then, all samples were immersed in black India ink for one week. After the storage period, the roots were then grooved longitudinally and split, and the ink penetration was measured from the apical part to the coronal part of the root canal into which the ink penetrated using a stereomicroscope. Data were analyzed by one-way ANOVA and Tukey's post-hoc tests. Results: There was no statistical difference in ink penetration between the different endodontic sealers tested for the same irrigating solution (p > 0.05). However, when the Bio-C Sealer (Angelus) was used, the group treated with 2.5% Ca(OCl)2 was associated with lower values of apical leakage, compared to 2.5% NaOCl (p < 0.05). For the AH Plus sealer (Dentsply Maillefer), there was no difference between the irrigants (p > 0.05). Conclusions: Associating Ca(OCl)2 irrigant with Bio-C Sealer (Angelus) seems to be a good option to reduce apical leakage

Efeito do hipoclorito de cálcio na resistência de adesão do cimento obturador AH Plus à dentina / Effect of calcium hypochlorite on the bond strength of the AH Plus sealer to dentin

Objetivo: avaliar o efeito do uso de hipoclorito de cálcio (Ca(OCl)2) como irrigante na resistência de adesão do cimento AH Plus (De Trey-Dentsply, Konstanz, Alemanha) à dentina pelo teste de micro push-out. Materiais e método: trinta e três dentes humanos monorradiculares foram seccionados transversalmente na junção amelocementária e divididos em três grupos: hipoclorito de sódio (NaOCl) 2,5%, hipoclorito de cálcio 2,5% e soro fisiológico. Os canais foram preparados, irrigados ao final com EDTA 17% e obturados com cones de guta percha e cimento AH Plus. Após armazenagem por 7 dias, em 100% de umidade e a 37°C, os dentes foram seccionados transversalmente ao longo do eixo da raiz. Foram obtidas três fatias de cada dente (n=33), que foram submetidas ao ensaio de push-out. O tipo de falha foi analisado por fractografia e classificado em falha adesiva, coesiva ou mista. Os valores de resistência de união foram analisados pelo teste de Kruskal-Wallis, com nível de significância de 95%. Resultados: o grupo Ca(OCl)2 2,5% apresentou a menor média de resistência de adesão, diferindo estatisticamente do NaOCl e do soro fisiológico (p<0,05). Não houve diferença estatística entre os grupos irrigados com soro e NaOCl (p>0,05). Conclusões: a falha predominante em todos os grupos foi a do tipo adesiva. O Ca(OCl)2 2,5% teve um efeito negativo sobre a força de adesão do AH Plus à dentina radicular quando comparado ao NaOCl 2,5%. (AU)

Objective: to evaluate the effect of calcium hypochlorite (Ca(OCl)2) as an irrigant on the bond strength of the AH Plus sealer (De Trey-Dentsply, Konstanz, Germany) to dentin, using the micro push-out test. Materials and method: thirty-three single-rooted human teeth were cross-sectioned on the cementoenamel junction and divided into three groups: 2.5% sodium hypochlorite (Na- OCl), 2.5% calcium hypochlorite, and saline solution. The canals were prepared, irrigated with 17% EDTA at the end, and filled with gutta-percha cones and AH Plus sealer. After being stored for seven days at 100% humidity and 37ºC, the teeth were cross-sectioned along the root axis. Three slices of each tooth (n=33) were obtained and subjected to the push-out test. Failure mode was analyzed by fractography and classified as adhesive, cohesive, or mixed. Bond strength values were analyzed by the Kruskall-Wallis test at 95% significance level. Results: the 2.5% Ca(OCl)2 group showed the lowest bond strength mean, differing statistically from 2.5% NaOCl and saline solution (p<0.05). There was no statistical difference between the groups irrigated with saline solution and NaOCl (p>0.05). Conclusion: the adhesive failure was predominant in all groups. The 2.5% Ca(OCl)2 had a negative effect on the bond strength of AH Plus to the root dentin when compared to 2.5% NaOCl. (AU)

Effect of chemical and natural irrigant solutions on microhardness of root dentin­ an in vitro study

Aim: Modifications in the mechanical properties of dentin may reduce the fracture resistance of tooth, especially after endodontic treatment. The aim of present study was to evaluate the effect of the irrigation with different root canal irrigants on the microhardness of root dentin. Methods: The coronal portion of 60 single-rooted bovine incisors was sectioned and the pulpal tissue removed using endodontic K-files. The roots were cut transversely to obtain 2 fragments, which were embedded in acrylic resin and randomly distributed into six groups (n=20) according to the irrigation protocol: distilled water (DW) (control); 2% chlorhexidine solution (CHX); 6% sodium hypochlorite (NaOCl); 6% calcium hypochlorite (Ca[OCl]2); QMix; and 6.5% grape seed extract solution (GSE). The solutions were kept in contact with the root dentin specimens for 30 min. After that, irrigation with 5 mL of DW was performed. The Vickers microhardness was determined by performing three indentations in all specimens, using 300-g load and 20-second dwell time. The first indentation was made 1.000 µm from the root canal entrance, and two other indentations were made at a distance of 200 µm from each other. The microhardness value for each specimen was obtained as the average of the results for the three indentations. Data were statistically analyzed using one-way ANOVA with 5% significance level. Results: All the tested irrigant solutions maintained the same microhardness level of the root dentin when compared to the control group, with no statistically significant differences between them (p<0.05) Conclusion: The tested irrigant solutions did not present ability to modify the microhardness of root dentin

Índices de calidad de agua del río Cucabaj ubicado en el municipio de Santa Cruz del Guiché, Guiché y la influencia en los costos de tratamientos de potabilización / Water quality index of cucabaj river located in the municipality of Santa Cruz del Quiché, Quiché and influence in treatment costs purification

El río Cucabaj es una de las fuentes principales de agua que abastece junto a los ríos Tabil y Aguacate a 1,352 hogares del área urbana del municipio de Santa Cruz del Quiché. Este rio recorre parte de los municipios de Santa Cruz del Quiché y Chiché. Es tributario del río Durazno y pertenece a esta microcuenca. En el periodo de estudio el caudal osciló entre 0.009-0.1004 m3 /seg. Gran proporción de este caudal se capta y recorre alrededor de 9 km. hacia la planta de tratamiento para su potabilización ubicada en la salida a San Pedro Jocopilas. La presente investigación tiene como objetivo mostrar la variabilidad temporal del recurso hídrico, determinando el índice de calidad del agua e índice de contaminación del río Cucabaj, así como los costos de desinfección utilizando hipoclorito de calcio. El monitoreo del rio fue realizado por un periodo de diez meses, de abril 2012 a enero 2013, según el índice de calidad de agua que muestra en una escala de 0-100 puntos, que suman los resultados de nueve parámetros y clasifica el agua del río Cucabaj en la categoría de media a buena (contaminada a levemente contaminada para uso humano, por lo que antes de su consumo debe de aplicarse tratamientos de potabilización). El mes con mayor caudal fue septiembre con 0.1004 m3/seg, particularidad que influyó considerablemente en presentar el valor más bajo en calidad y uno de los valores más altos en contaminación por materia orgánica. Esta característica se asocia principalmente a la presencia de coliformes totales. En cuanto a los costos de desinfección por hipoclorito de calcio, no se establecieron diferencias significativas en función de la cantidad de caudal.

Cucabaj River is a major source of water supplies along the Aguacate and Tabil Rivers to 1,352 households in the urban area of Santa Cruz del Quiché. This river runs through the municipality of Santa Cruz del Quiché and Chiche communities. It is a tributary of Durazno River and belongs to this watershed. In the study period the rate ranged from 0.009 - 0.1004 m3/s. A large proportion of this flow is captured and about 9 km transported to the treatment plant for purification in located in San Pedro Jocopilas. This investigation aims to show the temporal variability of water resources, determining the rates of water quality and pollution index Cucabaj River, as well as the cost of disinfection by means of calcium hypochlorite. The measurements were performed for a period of ten months, from April 2012 to January 2013. According to the water quality index which is a 100-point scale resulting of a total of nine different measurements, it was determined that the water quality of Cucabaj river is classified as moderate to good (which means that is slightly polluted to polluted for human use, so should receive purification treatment before consumption). The month with highest flow was September with rate 0.1004 m3/s, a feature that greatly influenced present the lowest value in quality and one of the highest values in organic pollution. This feature is mainly associated with the presence of total coliforms. As for costs calcium hypochlorite disinfection, no significant differences in terms of the amount of flow over other months were established.