Résumé
A trypsinized preparation of Mycobacterium phlei, non specific stimulator of immunity (NSI), and Sheep Pox Virus (SPV) were inoculated in different groups of sheep to activate B-lymphocytes and induce SPV neutralizing substance(s). NSI sensitized sheep B-lymphocytes in the presence of NSI or lymphokine elaborated SPV neutralizing substance(s). The SPV sensitized B-lymphocytes also mediated such neutralizing substance(s). Healthy control sheep B-lymphocytes failed to show any appreciable amount of viral neutralizing substance. However, a significant virus neutralizing substance(s) was detected when healthy sheep B-lymphocytes were cultured in presence of NSI antigen along with lymphokines.
Sujets)
Animaux , Antigènes de différenciation des myélomonocytes/immunologie , Antigènes néoplasiques , Lymphocytes B , Molécules d'adhérence cellulaire , Femelle , Immunité cellulaire , Activation des lymphocytes , Mâle , Glycoprotéines membranaires/immunologie , Mycobacterium phlei/immunologie , Poxviridae/immunologie , Infections à Poxviridae/immunologie , Ovis , VaccinationRésumé
Piglets treated with cyclophosphamide (50 mg/kg body wt, iv) before infection/vaccination with swinepox virus showed a strong suppression of humoral immune response. Cell mediated immune response was also affected to some extent and the cyclophosphamide treated piglets did not resist the challenge on 21 day post infection/vaccination and developed mild lesions.