Virological and pathological studies on avian encephalomyelitis among young chicks at El-Qassiem Area, Saudi Arabia

A history of nervous disturbances and moralities [25%] among young chickens 10-21 day of age as well as a temporary drop in egg production and hatchability [10%] in parent flock occurred in a large poultry farm at EL-Qassiem area, kingdom of Saudi Arabia. Detection, isolation and identification of avian encephalomyelitis [AE] virus was done using embryonated chicken eggs, immunoflourscence [IF] and Immunodiffusion [ID] tests. Serological investigations were carried out on infected sera from both parent flocks and broiler chicks using Enzyme-linked immunosorbant assay [ELISA]. Histopathological studies were carried out on samples from naturally infected chick brains as well as brains from inoculated hatched chicks

Detection of enteroviral sequence in endomyocardial tissues from patients with cardiac diseases in northern Brazil

No presente trabalho, são mostrados resultados de um estudo piloto direcionado à detecção de seqüências de enterovirus em tecido cardíaco obtido a partir de biópsias endomiocárdicas de pacientes com doenças cardíacas na região Amazônica. Seis amostras coletadas de três pacientes foram analisadas por RT-PCR obtendo-se três espécimes positivos e três negativos. Esses achados preliminares sugerem a participação dos enterovirus na etiologia de doenças cardíacas, principalmente miocardites, e justificam estudos mais amplos nesse assunto.

In the present report we describe the results from a pilot study aimed at detecting enterovirus sequence in cardiac tissues, obtained through endomyocardial biopsies, from patients suffering from cardiac diseases in the Amazon region. Six samples that were collected from three patients were analysed by RT-PCR showing 3 positive and 3 negative results. These preliminary findings suggest the participation of enteroviruses in the etiology of cardiac diseases, mainly myocarditis, and warrant further and broader local studies on this subject.