Cytochemical study of aryl sulphatase and non-specific esterase in colon adenocarcinoma after 5-fluorouracil and OCNU treatment and their effects on the immune function

The localisation and enzymatic activity of aryl sulphatase and non-specific esterase in normal colon, colon adenocarcinoma, 5-fluorouracil and CCNU treated cells were studied respectively. Intracytoplasmic granular reactions were observed for all tissues. The enzymatic activity was found to be more intense in malignant cells of colon. The increased activities of these enzymes in treated cells might be considered as an index of the in creased fragility of lysosomes and degeneration of some tumour cells which acompanied by a decrease in T-Cells function