Hypertonic glucose solution 10 percent - 25 percent on the mesenterium and peritoneum of the rat: macroscopic and microscopic study

OBJETIVO: Investigar as alterações macroscópicas e microscópicas do mesentério e do peritônio parietal quando se administra a solução aquosa de glicose hipertônica a 10% e a 25% na cavidade peritoneal de rato.MÉTODOS: 90 ratos fêmeas (n=90), adultos, "Wistar", jovens, com peso variando de 180 a 250 gramas foram divididos em 3 sub-grupos (A, B e C) contendo cada um 30 animais com procedimentos idênticos, diferindo apenas no período de observação. Os números de 1 a 30 constituem o grupo A ou grupo-controle (NaCl 0,9%), os números de 31 a 60 constituem o grupo B ou grupo-glicose a 10% e os números de 61 a 90 constituem o grupo C ou grupo- glicose a 25%. Realizando-se posteriormente laparotomia com incisão mediana longitudinal de pele a 2 cm abaixo do processo Xiphoideus sterni, estendendo-se por 3 cm caudalmente na linha média ventral. A escolha do procedimento a ser realizado para introdução na cavidade peritoneal de 2 ml de uma solução de cloreto de sódio 0,9% (controle), de glicose hipertônica a 10% e de glicose hipertônica a 25%. Em períodos correspondentes às 6h, 24h e 48h de pós-operatório, os animais de cada grupo foram reoperados, sendo realizada avaliação macroscópica e microscópica além dos registros das alterações histológicas do mesentério e peritônio parietal.RESULTADOS: Na microscopia do mesentério observou-se que 30 animais (33,4%) apresentaram linfonodos hiperplásicos; 6 animais (6,6%) com fibrose reacional; 10 animais (11,1%) com intensa congestão vascular; 16 animais (17,8%) com inflamação crônica inespecífica; 28 casos (31,1%) sem alteração. A microscopia do peritônio revelou 6 casos com fibrose reacional (3,3%) 174 casos (96,7%) sem alteração histológica. CONCLUSÃO: As soluções de glicose a 10% e a 25% não causam necrose tecidual quando introduzidas na cavidade peritoneal. O processo reacional inflamatório é de igual intensidade tecidual comparando-se ao uso da solução de NaCl a 0,9%.

Aspectos ultraestruturais da permeabilidade do mesentério de rato a exposiçâo ao ferro coloidal / Ultrastructural aspects of permeability in mouse mesentery expossed to coloidal iron

In previous we could observe that many researches were interested in the phenomena of absorption that occur in the mesothelial that involves the viscerae. We already made some researches empliying some chemical substances like the lanthaum nitrate and the osmium amine. With the utilization of the lanthanum nitrate we came to the conclusion that the penetration of this heavy salt is impeded by the presence of the basement membrane that separates the mesothelial from the connective tissue. This membrane, as observed at that time, is continous that separates the mesothelial from the connective tissue. This membrane, as observed at that time, is continous with the basal lamina located between mesothelial cells. Our observations led us to the conclusion that the salt traveled through the cytoplasm and not in between neighbouring mesothelial cells. The observations made in the material treated with osmjium-amine, were comapred to those made with the employment of the lanthanum nitrate. In general, no reaction was observed in any of the techniques employed, with the exception of some special cases. Int he later the osmium amine reacts in the outer surface of the mesentery but also penetates with no visible reaction, all the way to the connective tissue to react with the elastic layer. In the present paper, where tha colloidal iron was employed, int he mesothelial cells and in the connective tissue. We are comming to a final conclusion that the permeability of different layears of tissues is of a great variety and of a great capacity of selectiveness

Electron Microscopic Observations on the Morphological Changes of Rat Mesentric Mast Cells Induced by Morphine HCl

The effects of morphine HCI on the rat mesenteric mast cells were studied with the electron microscopy. The materials were prepared for electron microscopy by osmium tetroxide fixation and embedding in Epon. The rat mesenteric mast cells showed no distinct morphological changes due to morphine HCl, but the mast cell granlues were changed in various ways. For instance, they formed dusters, showed granular lysis, and an appearance of electron transparency. Frequently, some granules appeared in the extracellular space and the boundary of the granules was not evident. From the results mentioned above, it was suggested that rat mesenteric mast cell granules were affected by morphine HCl in the shape, the granular matrix, and the granular boundaries.

On the Degranulation of Rat's Mesenteric Mast Cells Caused by Morphine and Meperidine in Vitro

Histological studies were carried out on the degranulation of mesenteric mast cells of albino rats in which excised pieces of rat mesentery were incubated in media containing morphine and meperidine hydrochloride. The following conclusions were obtained. 1. The experimental dose of 0.04mg./ml. of morphine hydrochloride in Tyrode solution for the incubated mesenteric pieces brought about the degranulation of mast cells. 2. The experimental dose of 0.04mg./ml. of meperidine hydrochloride in Tyrode solution for the incubation of the mesenteric pieces did not effect the cytological changes of the mast cells. 3. By the addition of metabolic inhibitor such as iodoacetic acid to the incubating medium the degranulation of the mast cells was remarkably inhibited for the group in which the incubation was carried out for 20 minutes. However, the inhibition of the degranulation of the mast cells due to the metabolic inhibitor was abolished after 30 minutes of incubation. Consequently the authors have demonstrated the effect of morphine hydrochloride in its ability to induce a degranulation of mesenteric mast cells in vitro.

On the Effect of Morphine Hydrochloride on the Mesenteric Mast cells of Albino Rats

Histological studies were carried out on the degranulation of mesenteric mast cells of white rats caused by injections of morphine and nalorphine hydrochloride intravenously and the following conclusions were obtained. 1. By the injection of morphine hydrochloride fairly significant degranulation of the mesenteric mast cell was observed. 2. In various experimental doses of morphine hydrochloride the cytological change of the degranulation was not proportional to the doses of it in cases given more than 12mg./kg. of body weight. 3. The degranulating effect of the mesenteric mast cell by the injection of morphine hydrochloride was significantly inhibited by an adrenalectomy.

On the Degranulation of Mesenteric Mast Cells Caused by Morphine and Meperidine Hydrochloride in White Rats

Histological studies were carried out on the degranulation of mesenteric mast cells of white rats caused by injections of morphine hydrochbride and meperidine hydrochbride intravenously, intraperitoneally, and by local injection of the rat's mesentery and the following conclusions were obtained. 1. In the groups of intravenous, intraperitoneal, and local injections of morphine hydrochloride, fairly significant degranulation of the mesenteric mast cell was observed, which was probably associated with the concomitant liberation of tissue histamine derived from its source. 2. In the groups of intravenous and intraperitoneal injections of meperidine hydrochbride, the significant degranulation of the mesenteric mast ,cell was recognized. However, the local injections displayed no cytological change of the cell and no increased permeability of dermal capillaries was observed at the injecting site. 3. The degranulation of the mesenteric mast cell followed by an administration of meperidine hydrochloride was effectively inhibited after an adrenalectomy.