Résumé
A method was evaluated for determination of twenty-one doping (including nandrolone, boldenone and methandienone) in human urine by gas chromatography-high resolution mass spectrometry. Samples were prepared by liquid-liquid extraction, concentrated, TMS derivatization and limit of detection at ng x mL(-1) by MID/GC/HRMS. According to the code of the World Anti-Doping Agency (WADA), precision and recoveries of the procedure were evaluated by replicate analysis (n = 6), the recoveries in the range of 66%-103%, with the RSD below 10.0%. The precision within the day of the method with three different concentrations was also determined RSD were less than 9.5%, 10.0% and 9.7%.
Sujets)
Humains , Anabolisants , Urine , Dopage sportif , Chromatographie gazeuse-spectrométrie de masse , Métandiènone , Urine , Nandrolone , Urine , Détection d'abus de substances , Méthodes , Testostérone , UrineRésumé
The influence of anabolic steroid on the process of production of rickets in vitamin-D deficient animals as well as its influence on healing of rickets with vitamin-D therapy was studied. Long-Evans strains of female rats of two age groups (6 weeks and 4 weeks) were given rachitogenic diet and were divided into five sub-groups-(I) control receiving vitamin-D from the 1st day, (II) rachitic group, (III) receiving vitamin-D from 36th day, (IV) receiving anabolic hormone from the beginning of the experiment and (V) receiving both vitamin-D and anabolic hormone from 36th day of treatment. It was observed grossly as well as histologically and radiographically that: (1) the anabolic steroid minimizes the rachitic changes in the bones of vitamin-D deficient rats, (2) in rachitic animals, a combination therapy of anabolic hormone and vitamin-D results in comparatively greater calcification of osteoid matrix and better healing and remodelling of bone than with vitamin-D alone.
Sujets)
Animaux , Association de médicaments , Épiphyses (os)/anatomopathologie , Femelle , Métandiènone/usage thérapeutique , Rats , Rachitisme/traitement médicamenteux , Tibia/anatomopathologie , Vitamine D/usage thérapeutiqueSujets)
Adolescent , Adulte , Anthropométrie , Appétit , Bras/anatomie et histologie , Pression sanguine , Poids/effets des médicaments et des substances chimiques , Études de suivi , Humains , Mâle , Métandiènone/pharmacologie , Effort physique , Pouls , Épaisseur du pli cutané , Cuisse/anatomie et histologieRésumé
Se presenta un paciente de seis años de edad, afectado de anemia hipoplástica congénita. Se efectúa un análisis de la etiopatogenia, diagnóstico, pronóstico y de las posibilidades terapéuticas.