Résumé
Leptospirosis is a zoonosis caused by Leptospira interrogans. This disease is diagnosed by quantification of specific immunoglobulins in serum by the microagglutination test (MAT). The aims of this research were: a) to compare the protein profiles of 3 clinical isolates of bovine leptospirosis with the reference strain used for the MAT, and b) to identify the immunodomain antigens of the regional isolates through PAGE and immunoblotting techniques of bovine sera from infected, vaccinated and MAT-negative animals. Coomassie-blue stained gels revealed extensive protein similarities between pathogenic and reference strain. Most infected (8/10) and vaccinated animal sera (4/7) showed by immunoblotting a similar reactivity against the proteins from pathogenic leptospires, with a strong band of 25-30 kDa which was not detected in the reference strain. The lack of correlation between MAT and immunoblotting techniques for infected animals could be due either to the infection stage at which the diagnosis was made or to the immunoglobulin isotype involved in the response. Results obtained would confirm the antigenic differences between the 3 isolates and the reference strain.
Sujets)
Animaux , Bovins , Variation des antigènes , Antigènes bactériens/immunologie , Maladie de Weil/médecine vétérinaire , Maladies des bovins/microbiologie , Leptospira interrogans , Tests d'agglutination , Spécificité des anticorps , Anticorps antibactériens/sang , Anticorps antibactériens/immunologie , Antigènes bactériens/isolement et purification , Argentine , Vaccins antibactériens , Technique de Western , Maladie de Weil/sang , Maladie de Weil/microbiologie , Maladies des bovins/sang , Électrophorèse sur gel de polyacrylamide , Leptospira interrogans , Protéines bactériennes/immunologie , Protéines bactériennes/isolement et purification , Normes de référence , Coloration et marquageRésumé
A case of renal icterohemorrhagic leptospirosis involving a patient with acquired immunodeficiency syndrome (AIDS) is reported. Despite the low levels of CD4+ T lymphocytes, the clinical course of leptospirosis was similar to that observed in non-immunodepressed patients, and no worsening of AIDS occurred due to the infection by the spirochete. Serologic conversion was observed in the microscopic agglutination test, with maximum titer of 1:3,200. The patient had positive urine cultures for Leptospira interrogans for two months, whereas blood cultures were negative
Sujets)
Humains , Mâle , Maladie de Weil/diagnostic , VIH-1 (Virus de l'Immunodéficience Humaine de type 1) , Infections opportunistes liées au SIDA/diagnostic , Insuffisance rénale/diagnostic , Maladie de Weil/microbiologie , Maladie de Weil/anatomopathologie , Issue fatale , Infections opportunistes liées au SIDA/microbiologie , Infections opportunistes liées au SIDA/anatomopathologie , Leptospira interrogans/isolement et purification , Insuffisance rénale/microbiologie , Insuffisance rénale/anatomopathologieRésumé
Las fiebres recurrentes son producidas por espiroquetas del genero Borrelia y transmitidas a otros por garrapatas y piojos. Nosotros hemos estudiado la sensibilidad y especificidad de un micrometodo de concentracion para el diagnostico de la Borreliosis en sangre. Se tomo sangre de ratones que fueron inoculados previamente con una cepa de Borrelia de origen humano. Tubos de micro-hematocrito fueron llenados con sangre, centrifugados y observados a traves del microscopio. La observacion se realiza en la interface de los globulos rojos y el plasma. El micrometodo se muestra mas sensible cuando los niveles de bacteremia son bajos