Résumé
Currently in Colombia, there are only records of morph-agronomic characterizations of Mangifera indica cvar. Hilacha; molecular studies on this mango variety have not been carried out. The aim of this work was to identify the genetic diversity of six populations of mango Hilacha by RAPDs markers, as a fundamental base for breeding programs, conservation and selection of promissory materials for the fruit industry at the national level. From 60 primers evaluated in the populations, five primers were selected and were launched in the six populations. Polymorphic bands of RAPDs were transformed into binary matrices, which were then processed with NTSYS-PC, POPGENE and TFPGA softwares. The overall genetic diversity, H T = 0.468 +/- 0.0016, is very similar to the average subpopulation genetic diversity, H S = 0.4431 +/- 0.0024, which revealed a small genetic differentiation among the mango Hilacha populations studied (G ST = 0.0532). This means that each population contained in average 95 percent of the total genetic diversity found in the global population analyzed. Considerable gene flow between populations (Nm = 9) was found. Finally, we recommend studying the genetic diversity of mango Hilacha populations with other molecular markers to complement the information obtained and to find similarities or differences with the results presented herein.
Sujets)
Mangifera/anatomie et histologie , Mangifera/classification , Mangifera/génétique , Mangifera/composition chimique , Variation génétique/génétique , Colombie , Marqueurs génétiques , Technique RAPDRésumé
O experimento foi conduzido na base física da Empresa de Pesquisa Agropecuária do Estado do Rio Grande doNorte (EMPARN) em Açu-RN, com o objetivo de avaliar o comportamento de cultivares copas em função de diferentes portaenxertos. O delineamento experimental utilizado foi em blocos casualizados num esquema fatorial 3 x 4, correspondentes às cultivares de enxertos e porta-enxertos respectivamente, e três repetições. Como porta-enxertos foram utilizadas os cultivares Carabao e Manga dágua, provenientes do Instituto Agronômico de Campinas (IAC), e os cultivares Rosinha e Espada, obtidas da coleção da EMPARN. Os cultivares empregados como copas foram Tommy Atkins, Van Dyke e Keitt, oriundos da EMPARN. Semestralmente avaliaram-se os diâmetros dos porta-enxertos, enxertos e altura das plantas. O cultivar Manga dágua, como porta-enxerto, promoveu o menor crescimento às mangueiras e o cultivar Tommy Atkins, usado como enxerto, foi a que apresentou a maior altura.
The experiment was conducted at the physics structure of the Rio Grande do Norte State Farming ResearchCompany (EMPARN) in Açu-RN, with the objective of evaluate the behavior of grafts-holder cultivates in the plants formation of cultivates used as tops. The experimental design was randonizated blocks in the factorial outline 3 x 4, with 3 repetitions. The analyzed factors were four cultivates of grafts-holder and three cultivates of grafts. As grafts-holders were used the cultivates Carabao and Manga dagua, originated at the Campinas Agrarian Institute, and the cultivates Rosinha and Espada, obtained from the EMPARN collection. The cultivates used as tops were Tommy Atkins, Van Dyke and Keitt, with the fork that were also obtained at EMPARN. In a period of six months was observed the diameters of the grafts-holder, grafts and hight of the plants. The Cultivar Manga dagua, as a graft-holder, provided the smallest growing to the mango tree and the Cultivar Tommy Atkins, used as graft, was the one who presented the tallest hight.