Résumé
OBJECTIVE@#To explore the forensic application value of detection of matrix metalloproteinase-11 (MMP-11) in menstrual blood by enhanced chemiluminescence method.@*METHODS@#Menstrual blood, vaginal swab, peripheral blood, saliva stain, urine stain and semen stain were collected to detect whether or not there were MMP-11 using enhanced chemiluminescence method. The specificity and reliability of the MMP-11 assay along with its sensitivity were evaluated.@*RESULTS@#The positive detection rate of MMP-11 in menstrual blood was 89.47%, whereas no MMP-11 was found in vaginal swab, peripheral blood, saliva stain, urine stain and semen stain. When 25 microL sample was added, the mass concentration of protein was 1.329 microg/microL, then MMP-11 could be detected. A positive detection rate of 89.58% was observed in MMP-11 positive menstrual blood samples after stored at 4 degrees C for 20 months.@*CONCLUSION@#Enhanced chemiluminescence method is sensitive and specific for detecting MMP-11, and can be applied to distinguish menstrual blood from common stain such as peripheral blood, vaginal fluid.
Sujets)
Femelle , Humains , Marqueurs biologiques/sang , Taches de sang , Technique de Western , Médecine légale/méthodes , Mesures de luminescence/méthodes , Matrix metalloproteinase 11/sang , Menstruation , Reproductibilité des résultats , Salive/composition chimique , Sensibilité et spécificité , Urine/composition chimique , Vagin/composition chimiqueRésumé
OBJECTIVE@#To prove the feasibility of detecting menstrual blood as well as its cellular localization with rabbit-anti-human matrix metalloproteinase-11 (MMP-11) polyclonal antibody.@*METHODS@#MMP-11 in menstrual blood, peripheral blood, vaginal liquid, aged menstrual bloodstain, and endometrium sections were assayed with SAP immunohistochemistry.@*RESULTS@#MMP-11 was found only in menstrual samples within stroma and epithelium cells.@*CONCLUSION@#MMP-11 polyclonal antibody may be applied in the distinction between menstrual blood and venous blood.
Sujets)
Adulte , Femelle , Humains , Endomètre/anatomopathologie , Médecine légale/méthodes , Immunohistochimie , Matrix metalloproteinase 11/analyse , Cycle menstruel/sangRésumé
OBJECTIVE@#To investigate the role of expression of stromelysin-3 in laryngeal carcinoma and the relationship between stromelysin-3 expression and the invasion and metastasis of laryngeal cancer.@*METHOD@#The expression of stromelysin-3 in 63 samples with laryngeal carcinoma and 63 adjacent normal tissue were detected by immunohistochemistry. The mRNA expression of stromelysin-3 were examined by reverse transcript-polymerase chain reaction (RT-PCR) in 22 frozen specimens of laryngeal cancer and 22 adjacent normal tissue.@*RESULT@#The immunochemistry showed the expression of stromelysin-3 in laryngeal cancer is much higher than that in adjacent normal tissue (P 0.05).@*CONCLUSION@#The expression of stromelysin-3 was closely associated with the invasion and metastases of laryngeal carcinoma, it may be served as a marker in estimating the invasion and metastases potency of laryngeal cancer.
Sujets)
Adulte , Sujet âgé , Femelle , Humains , Mâle , Adulte d'âge moyen , Marqueurs biologiques tumoraux , Tumeurs du larynx , Métabolisme , Anatomopathologie , Métastase lymphatique , Matrix metalloproteinase 11 , Métabolisme , Invasion tumoraleRésumé
The identification of menstrual blood belongs to the forensic examination of bloodstains. The traditional methods for detecting menstrual blood were reviewed and their obstacles in forensic application were discussed. Matrix metalloproteinase-11 is a kind of protease which degrades the extracellular matrix. Some researches had indicated that matrix metalloproteinase-11 might be a new marker for specifically and sensitively detecting menstrual blood. The structure, function of matrix metalloproteinase-11 and its application in menstrual blood identification were reviewed.