Atividade biológica do Lipopolissacarídeo (LPS) de Moraxella bovis

Foram avaliadas algumas propriedades biológicas do lipopolissacarídeo extraído de M. bovis. Experimentos realizados in vivo com o LPS purificado demonstraram toxicidade evidenciada pel DL50 em camundongos. Lesöes dermonecróticas localizadas foram detectadas pela reaçäo de Shwartzman em coelhos albino. Além disso, foi observada uma severa conjuntivite em camundongos que resistiram ao desafio intraperitoneal com o LPS sugerindo, desse modo, um importante mecanismo predispondo à penetraçäo de M. bovis na mucosa conjuntiva

Differentiation of nonfermentative gram-negative bacilli in the clinical laboratory.

A rapid and simplified system for the differentiation of nonfermentative Gram-negative bacilli, encountered frequently in clinical specimens, is presented for use in the clinical laboratory. Nonfermentative bacteria can be grouped initially by the motility, oxidase and OF glucose reactions. This grouping simplifies the choice of additional tests for further identification. The additional tests included Gram stain, acid production from 10% lactose agar, nitrate reduction, arginine dihydrolase activity, fluorescein production, deoxyribonuclease activity, hydrolysis of aesculin, growth at 42 degrees C, gelatinase activity and susceptibility to antibiotics.