Three Cases of Moraxella osloensis Meningitis: A Difficult Experience in Species Identification and Determination of Clinical Significance

We had three cases of Moraxella osloensis meningitis. The species identification was impossible by conventional and commercial phenotypic tests. However, we could identify the species using the 16S rRNA gene sequencing. Determination of clinical significance was difficult in one patient. All three patients recovered by appropriate antimicrobial therapy.

Bacterial flora of sturgeon fingerling

The study on microbial populations is a suitable tool to understand and apply control methods to improve the sanitary level of production in fish breeding and rearing centers, ensure health of sturgeon fingerlings at the time of their release into the rivers and also in the conversation and restoration of these valuable stocks in the Caspian Sea, Iran. A laboratory research based on Austin methods [Austin, B., Austin, D.A 1993] was conducted for bacterial study on 3 sturgeon species naming A. persicus, A. stellatus and A. nudiventris during different growth stages. Bacterial flora of Acinetobacter, Moraxella, Aeromonas, Vibrio, Edwardsiella, Staphylococcus, Proteus, Yersinia, Pseudomonas and Plesiomonas were determined. The factors which may induce changes in bacterial population during different stages of life are the followings: quality of water in rearing ponds, different conditions for growth stages, suitable time for colonization of bacterial flora in rearing pond, water temperature increase in fingerlings size and feeding condition

Invasive infection due to Moraxella lacunata, an unusual pathogen.

Moraxella lacunata, a commensal bacterium, is associated with serious invasive disease. We describe a patient with diabetic nephropathy who developed septicemia with metastatic abscesses in the liver and spleen due to Moraxella lacunata. The patient also had multiple ring enhancing lesions in both the cerebral hemispheres, possibly due to the same organism.

Bacterial causative agents of conjunctivitis and keratoconjunctivitis in cattle, sheep and goat and survey of pathogenicity and immunogenicity of Moraxella species

Bacterial culture of eye secretion from bovine and ovine infected eyes was done during a period of three years [1992-1994]. Moraxella bovis [M. bovis] in bovine and Branhamella ovis [B.ovis] in ovine cases were the most isolates. M. bovis was isolated from 72 of a total of 132 bovine cases [54.54%]. The other isolations were staphylococcus sp., streptoccus sp. Pseudomonas aeroginosa and non pathogenic moraxella sp. In sheep and goat B ovis was isolate from 7 cases of a total of 17 [41%] and two cases were non pathogenic moraxella sp. This observation indicates the high incidence of pathogenicity of B. ovis in these animals however the mechanism of bacterial infection is still unknown. Since Chlamydia and rickettsiae are tought to be responsible for primary conjunctivitis two methods of staining were employed to observe these organisms in eye epithelial cell: 1] Macchiavello staining and 2] modified Ziehl Nelson staining. Only two cases of cattle infected eyes showed chlamidial bodies with second staining method

Produçäo de enterotoxina por Moraxella bovis

Sete amostras de Moraxella bovis foram investigadas quanto a produçäo de exotoxina. os resultados observados em quatro delas (57,14%) mostraram que M. bovis é capaz de induzir no modelo do camundongo recém-nascido um efeito semelhante ao observado para a toxina termoestável secretada por Escherichia coli

Atividade biológica do Lipopolissacarídeo (LPS) de Moraxella bovis

Foram avaliadas algumas propriedades biológicas do lipopolissacarídeo extraído de M. bovis. Experimentos realizados in vivo com o LPS purificado demonstraram toxicidade evidenciada pel DL50 em camundongos. Lesöes dermonecróticas localizadas foram detectadas pela reaçäo de Shwartzman em coelhos albino. Além disso, foi observada uma severa conjuntivite em camundongos que resistiram ao desafio intraperitoneal com o LPS sugerindo, desse modo, um importante mecanismo predispondo à penetraçäo de M. bovis na mucosa conjuntiva

Microorganismos patogenos de hallazgo poco comun en infectologia / Pathogenic microorganisms not frecuently found in infectology

Se estudiaron los microorganismos de identificacion dificil referidos al laboratorio de bacteriologia del Instituto Nacional de Salud por 5 Instituciones. En 70 casos fue posible obtener una identificacion completa y responsabilizar al microorganismo aislado como agente etiologico de un cuadro clinico. Se trataba de 28 cuadros de septicemia, 26 de meningitis, 4 respiratorios, 7 genitourinarios y 6 varios que incluyeron heridas, abcesos, y conjuntivitis. Los identificados fueron: Serratia en el 38%, Acinetobacter en el 21.4%, Moraxella en el 10%, Alcaligenes en el 8.7%, Aeromonas en el 7.2%, Listeria en el 5.7%, Estreptobacilos en el 4.3% Corynebacterium en el 4.3%, Achromabacter, Cardiobacterium y los grupos M3 y M4 aparece cada uno con el 1.4% constituyendose en verdaderas curiosidades biologicas