Résumé
Mycoplasma spp, belongs to the class Mollicutes and is capable to produce alterations in cellular cultures causing damages to the biotechnological industry. Bioproducts generally require two essential inputs, bovine serum and cells. The study herein aims to evaluate the mycoplasma concentrations that affect the growing of BHK21 and Vero cells. The species used were: Mycoplasma orale, M. salivarium, M. arginini and M. hyorhinis, cultivated in a SP4 media. Two contamination tests were performed with BHK21 and Vero cells and one of them applied different concentrations of mycoplasma. In the first one, mycoplasma was applied at the day zero and, in the second one, the contamination was performed after the monolayer establishment. The both cellular cultures presented cytopathic effects with mycoplasma contamination, but the Vero cells suffered more damages than the BHK21 ones. It was also observed that the severity of the cytopathic effect depended on the mycoplasma specie, on the concentration and on the time of contact with the cellular culture, which evidences the importance of controlling the presence of mycoplasma in biotechnological industries.
Sujets)
Animaux , Cricetinae , Cellules épithéliales/microbiologie , Cellules épithéliales/physiologie , Mycoplasma/croissance et développement , Lignée cellulaire , Chlorocebus aethiops , Techniques de coculture , Milieux de culture/composition chimiqueRésumé
Se utilizó el método microcalorimétrico para estudiar el comportamiento de dos cepas de micoplasmas, responsables de la infección de cultivo de células animales. Los termogramas obtenidos permitieron realizar un análisis comparativo de diferentes parámetros entre estas especies, lo cual indica la aplicación ventajosa de este método sobre los métodos convencionales de detección del crecimiento para estos microorganismos