Your browser doesn't support javascript.
loading
Montrer: 20 | 50 | 100
Résultats 1 - 8 de 8
Filtre
1.
Biocell ; 34(3): 113-120, Dec. 2010. ilus, graf
Article Dans Anglais | LILACS | ID: lil-595012

Résumé

2-Methoxyestradiol-bis-sulphamate is a bis-sulphamoylated derivative of the naturally occurring 17-beta-estradiol metabolite namely 2-methoxyestradiol. 2-Methoxyestradiol-bis-sulphamate is regarded as a potential anticancer drug with increased antiproliferative activity when compared to 2-methoxyestradiol. The aim of this pilot in vitro study was to determine the influence of 2-methoxyestradiol-bis-sulphamate on cell growth, morphology and possible induction of certain types of cell death in the SNO esophageal carcinoma cell line. A dose-dependent study (0.2-1.0 microM) was conducted with an exposure time of 24 hours. Data revealed that 2-methoxyestradiol-bis-sulphamate reduced cell numbers statistically significantly to 74% after exposure to 0.4 microM of the drug. Morphological studies including light microscopy demonstrated hallmarks of apoptosis, while fluorescent microscopy revealed both the presence of apoptosis and autophagy as types of cell death being induced in SNO cells after 24 hours of exposure to 0.4 microM 2-methoxyestradiol-bis-sulphamate.


Sujets)
Humains , Apoptose , Autophagie , Numération cellulaire , Carcinome épidermoïde/anatomopathologie , Cycle cellulaire , Division cellulaire , Lignée cellulaire tumorale , Relation dose-effet des médicaments , Oestriol/analogues et dérivés , Oestriol/pharmacologie , Tumeurs de l'oesophage/anatomopathologie
2.
Biocell ; 34(2): 71-79, Aug. 2010. ilus, graf
Article Dans Anglais | LILACS | ID: lil-595041

Résumé

In the search for new and improved anticancer therapies, researchers have identified several potentially useful compounds. One of these agents is 2-methoxyestradiol-bis-sulphamate (2ME-BM), a sulphamoylated derivative of 2-methoxyestradiol. The objective of this study was to evaluate 2ME-BM's in vitro efficacy as antiproliferative agent in the MCF-7 breast adenocarcinoma cell line. Light- and fluorescent microscopy showed decreased cell density, increased apoptotic characteristics and significant ultrastructural aberrations indicative of autophagic cell death after 24 hours of exposure at a concentration of 0.4 microM. In addition, mitotic indices revealed that 2ME-BM induces a G2M block. The latter was confirmed by flow cytometric analyses where increased sub-G1 and G2/M fractions, as well as an increase in cycli n B1 levels were observed. Further in vitro research into the mechanism of this potentially useful anticancer compound is thus warranted.


Sujets)
Humains , Femelle , Cycle cellulaire , Oestriol/analogues et dérivés , Oestriol/pharmacologie , Oestriol/composition chimique , Lignée cellulaire tumorale , Lignée cellulaire tumorale/ultrastructure , Prolifération cellulaire , Tumeurs du sein , Structure moléculaire
3.
São Paulo med. j ; 125(6): 343-350, Nov. 2007. ilus, tab
Article Dans Anglais | LILACS | ID: lil-476094

Résumé

CONTEXT AND OBJECTIVE: Mammary fibroadenoma is a disease that affects a large number of women of reproductive age. The aim of this study was to evaluate the proliferative activity of mammary fibroadenoma through expression of Ki-67 and c-myc antigens, following administration of oral contraceptive with or without estriol. DESIGN AND SETTING: Placebo-controlled double-blind randomized clinical trial in the Mastology Sector of the Department of Gynecology, Universidade Federal de São Paulo. METHODS: Thirty-three fibroadenoma patients were studied. Ten women (group 1) took an oral contraceptive constituted by levonorgestrel and ethinyl estradiol together with placebo manufactured in the same capsule for four consecutive cycles with a seven-day interval between them. The other 23 patients (group 2) took the same oral contraceptive together with estriol, which was put into the same capsule and used in the same way as among the group 1 patients. After four cycles, the nodules were surgically removed and sent for immunohistochemical analysis of Ki-67 and c-myc expression. RESULTS: The Ki-67 and c-myc analysis did not reveal any significant differences between the study groups. The values were 9.16 and 10.54 for group 1 and 10.86 and 17.03 for group 2, respectively. There was a tendency towards higher expression of antigens in group 2. CONCLUSION: Our results showed that there was no significant statistical difference in Ki-67 and c-myc expression between our study groups, but only a tendency towards higher expression among users of oral contraceptives containing estriol.


CONTEXTO E OBJETIVO: O fibroadenoma mamário é uma doença que atinge um grande número de mulheres na idade reprodutiva. O objetivo foi avaliar a atividade proliferativa do fibroadenoma mamário, através da expressão do Ki-67 e do c-myc, após a administração de anticoncepcional oral, associado ou não ao estriol. TIPO DE ESTUDO E LOCAL: Ensaio clínico randomizado, duplo-cego, placebo controlado, realizado na Universidade Federal de São Paulo a nível terciário. MÉTODOS: Foram estudadas 33 pacientes portadoras de fibroadenoma, atendidas no setor de Mastologia da Disciplina de Ginecologia da Universidade Federal de São Paulo - Escola Paulista de Medicina (Unifesp-EPM), sendo que 10 mulheres constituíram o grupo 1, e utilizaram anticoncepcional oral composto de levonorgestrel e etinilestradiol, associados a placebo na mesma cápsula por quatro ciclos consecutivos, com intervalo de sete dias entre cada um. As restantes 23 pacientes alocaram-se no grupo 2 e ingeriram, além do anticoncepcional oral descrito acima, um comprimido de estriol, que foi manufaturado na mesma cápsula e foi utilizado da mesma forma que nas pacientes do grupo 1. Ao final dos quatro ciclos, praticou-se a exérese cirúrgica dos nódulos, com posterior envio para análise imunoistoquímica de Ki-67 e c-myc. RESULTADOS: A análise com Ki-67 e c-myc não revelou diferença significante entre os grupos estudados, que foi de 9,16 e 10,54 no grupo 1 e de 10,86 e 17,03 no grupo 2, respectivamente, apesar de ter havido tendência a maior expressão dos marcadores entre as pacientes do grupo 2. CONCLUSÃO: Nossos resultados demonstram não haver diferença estatisticamente significante na expressão de Ki-67 e de c-myc entre os grupos em estudo, apenas uma tendência a sua maior expressão entre as usuárias de anticoncepcional e estriol.


Sujets)
Adulte , Femelle , Humains , Jeune adulte , Tumeurs du sein/anatomopathologie , Contraceptifs oraux combinés/pharmacologie , Cellules épithéliales/effets des médicaments et des substances chimiques , Oestriol/pharmacologie , Fibroadénome/anatomopathologie , /analyse , Biopsie , Tumeurs du sein/métabolisme , Prolifération cellulaire/effets des médicaments et des substances chimiques , Contraceptifs oraux combinés/effets indésirables , Méthode en double aveugle , Oestriol/effets indésirables , Éthinyloestradiol/effets indésirables , Fibroadénome/métabolisme , Gènes myc/physiologie , Immunohistochimie , /métabolisme , Lévonorgestrel/effets indésirables , Glandes mammaires humaines/effets des médicaments et des substances chimiques , Antigène nucléaire de prolifération cellulaire/métabolisme , Coloration et marquage , Jeune adulte
4.
Indian J Exp Biol ; 2007 Aug; 45(8): 676-82
Article Dans Anglais | IMSEAR | ID: sea-56708

Résumé

In vitro effects of gonadal hormones (testosterone, 17beta-estradiol estriol and estrone) and corticosteroid hormones (corticosterone and cortisol) were studied on arylalklyamine N-acetyltransferase (AA-NAT) activity in the pineal organ of the fish, C. gariepinus during quiescent, progressive, breeding and regressive phases of its annual breeding cycle. The pineals were collected under dim red light, maintained in organ culture for 7 hr and incubated with three concentrations (10(-6), 10(-5) and 10(-4) M) of hormones for 6 hr. The treatments with gonadal hormones and corticosteroid hormones inhibited pineal AA-NAT activity in a dose-dependent manner during all the phases of the breeding cycle. AA-NAT activity was comparatively more sensitive to the inhibitory effects of the gonadal hormones during the regressive phase and less sensitive during the quiescent phase. Further, the enzyme activity was more sensitive to the inhibitory effects of corticosteroid hormones (corticosterone and cortisol) during the breeding phase and less sensitive during the quiescent phase. These findings seem to suggest that gonadal hormones and corticosteroid hormones have direct inhibitory influence on AA-NAT activity and, hence melatonin synthesis in the photoreceptive pineal organ of C. gariepinus.


Sujets)
Hormones corticosurrénaliennes/pharmacologie , Animaux , Arylamine N-acetyltransferase/antagonistes et inhibiteurs , Sélection , Poissons-chats/croissance et développement , Oestradiol/pharmacologie , Oestriol/pharmacologie , Hormones sexuelles stéroïdiennes/pharmacologie , Mélatonine/métabolisme , Glande pinéale/effets des médicaments et des substances chimiques , Testostérone/pharmacologie
6.
Journal of Korean Medical Science ; : 277-285, 1999.
Article Dans Anglais | WPRIM | ID: wpr-10460

Résumé

The effects of estriol on oxygen uptake, glucose release, lactate and pyruvate production, beta-hydroxybutyrate and acetoacetate production in perfused rat liver as well as, carbon uptake in rat liver and intracellular calcium in isolated Kupffer cells were investigated. Basal oxygen consumption of perfused liver increased significantly in estriol or ethanol-treated rats. But these increased effects were blocked by gadolinium chloride pretreatment. In a metabolic study, pretreatment with estriol resulted in a decrease in glucose production and in glycolysis while an increase in ketogenesis. A more oxidized redox state of the mitochondria was indicated by increased ratios of perfusate [lactate]/[pyruvate] and decreased ratios of perfusate [beta-hydroxybutyrate]/[acetoacetate]. Carbon uptake of Kupffer-cell increased significantly in estriol-treated rats. But these increased uptake were not shown in rats pre-treated by gadolinium chloride blocking phagocytosis. In isolated Kupffer cells from estriol-treated rats, intracellular calcium was more significantly increased after addition of lipopolysaccharide (LPS) than in controls. These findings suggest that the metabolic effects of estriol (two mg per 100 mg body wt) can be summarized to be highly toxic in rat liver, and these findings suggest that oral administration of estrogens may induce hepatic dysfunctions and play a role in the development of liver disease.


Sujets)
Femelle , Rats , Acide 3-hydroxy-butyrique/métabolisme , Acétoacétates/métabolisme , Animaux , Calcium/métabolisme , Glucides/métabolisme , Carbone/métabolisme , Cellules cultivées , Colloïdes/métabolisme , Oestriol/pharmacologie , Oestriol/métabolisme , Éthanol/pharmacologie , Gadolinium/pharmacologie , Glucose/biosynthèse , Liquide intracellulaire/métabolisme , Cellules de Küpffer/métabolisme , Cellules de Küpffer/cytologie , Lactates/métabolisme , Lipides/métabolisme , Foie/métabolisme , Foie/effets des médicaments et des substances chimiques , Consommation d'oxygène , Phagocytose , Acide pyruvique/métabolisme , Rat Sprague-Dawley
SÉLECTION CITATIONS
Détails de la recherche