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1.
Rev. chil. infectol ; 40(4): 426-429, ago. 2023. ilus
Article Dans Espagnol | LILACS | ID: biblio-1521843

Résumé

La peritonitis asociada a diálisis peritoneal es una complicación grave en el paciente con este tipo de modalidad de terapia de reemplazo renal, siendo la etiología fúngica una de las que conlleva mayor morbimortalidad. Presentamos el caso de un paciente de 22 años que desarrolló una peritonitis asociada a diálisis peritoneal causada por el complejo Paecilomyces variotii; un hongo filamentoso poco frecuente en este grupo de pacientes.


Peritonitis associated with peritoneal dialysis is a serious complication in patients with this type of renal replacement therapy modality, with fungal aetiology being one of the most associated with morbidity and mortality. We present the case of a 22-year-old patient who developed fungal peritonitis associated with peritoneal dialysis caused by Paecilomyces variotii complex; a rare cause of peritonitis in this group of patients.


Sujets)
Humains , Mâle , Jeune adulte , Péritonite/étiologie , Dialyse péritonéale/effets indésirables , Mycoses/complications , Péritonite/traitement médicamenteux , Paecilomyces/génétique , Mycoses/traitement médicamenteux , Antifongiques/usage thérapeutique
2.
Genet. mol. biol ; 29(1): 132-136, 2006. ilus, tab
Article Dans Anglais | LILACS | ID: lil-424749

Résumé

A phylogenetic analysis of the 5.8S rDNA and internal transcribed spacer (ITS1 and ITS2) sequences from some entomogenous Paecilomyces species supports the polyphyly of the genus and showed the existence of cryptic species. In the Eurotiales, anamorphs Paecilomyces variotii and Paecilomyces leycettanus were related to the teleomorphs Talaromyces and Thermoascus. In the Hypocreales, three major ITS subgroups were found, one of which included Paecilomyces viridis, Paecilomyces penicillatus, Paecilomyces carneus and isolates identified as Paecilomyces lilacinus and Paecilomyces marquandii. However, the majority of the P. lilacinus and P. marquandii isolates formed a distinct and distantly related subgroup, while the other major subgroup contained Paecilomyces farinosus, Paecilomyces amoeneroseus, Paecilomyces fumosoroseus and Paecilomyces tenuipes.


Sujets)
Animaux , ADN fongique , ADN ribosomique , Paecilomyces/classification , Marqueurs génétiques , Phylogenèse , Paecilomyces/génétique
3.
Genet. mol. biol ; 22(1): 119-23, Mar. 1999. tab, ilus
Article Dans Anglais | LILACS | ID: lil-243520

Résumé

The entomopathogenic fungi Paecilomyces fumosoroseus and P. lilacinus have been transformed to resistance to the fungicide benomyl by a polyethylene glycol (PEG)-mediated procedure using a mutant b-tubulin gene from Neurospora crassa carried on plasmid pBT6. Benomyl-resistant transformants of P. lilacinus were obtained that could tolerate greater than 30 µg/ml benomyl and P. fumosoroseus transformants were obtained that could tolerate 20 µg/ml benomyl. Following 5 serial passages of transformants on benomyl-containing media and 5 serial passages on non-selective media, 100 per cent of P. lilacinus transformants were found to be mitotically stable by a conidial germination test. In contrast, only 4 out of 9 transformants of P. fumosoroseus were mitotically stable. Southern blot analysis of genomic DNA from both species suggested that the mechanism of transformation in all transformants was by gene replacement of the b-tubulin allele. Non-homologous vector sequences were not detectable in the genomes of transformants.


Sujets)
Benomyl/pharmacologie , Fongicides industriels/pharmacologie , Paecilomyces/effets des médicaments et des substances chimiques , Paecilomyces/génétique , Transformation génétique , Technique de Southern , ADN , Hybridation d'acides nucléiques , Plasmides , Résistance microbienne aux médicaments
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