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1.
J Biosci ; 2006 Jun; 31(2): 223-33
Article Dans Anglais | IMSEAR | ID: sea-110745

Résumé

A putative Hsp100 gene was cloned from the fungus Pleurotus sajor-caju. mRNA expression studies demonstrated that this gene (designated PsHsp100) is highly induced by high temperature,induced less strongly by exposure to ethanol, and not induced by drought or salinity. Heat shock induction is detectable at 37 degrees C and reaches a maximum level at 42 degrees C. PsHsp100 mRNA levels sharply increased within 15 min of exposure to high temperature, and reached a maximum expression level at 2 h that was maintained for several hours. These results indicate that PsHsp100 could work at an early step in thermotolerance. To examine its function, PsHsp100 was transformed into a temperature-sensitive hsp104 deletion mutant Saccharomycetes cerivisiae strain to test the hypothesis that PsHSP100 is an protein that functions in thermotolerance. Overexpression of PsHSP100 complemented the thermotolerance defect of the hsp104 mutant yeast, allowing them being survive even at 50 degree C for 4 h. These results indicate that PsHSP100 protein is functional as an HSP100 in yeast and could play and important role in thermotolerance in P. sajor-caju.


Sujets)
Séquence d'acides aminés , Séquence nucléotidique , Protéines fongiques/classification , Régulation de l'expression des gènes , Test de complémentation , Protéines du choc thermique/classification , Température élevée , Concentration en ions d'hydrogène , Données de séquences moléculaires , Organismes génétiquement modifiés , Phylogenèse , Pleurotus/génétique , ARN messager/métabolisme , Saccharomyces cerevisiae/génétique , Protéines de Saccharomyces cerevisiae/génétique , Alignement de séquences
2.
Rev. Soc. Bras. Med. Trop ; 28(4): 321-4, Oct.-Dec. 1995. tab
Article Dans Portugais | LILACS | ID: lil-187120

Résumé

The authors studied the killer effect of nine standard strains of yeasts on 146 samples of Candida albicans isolated from the following clinical specimens: oral mucosa, feces, bronchial wash, sputum, vaginal secretion, urine, skin lesion, nail lesion and blood. Using this system it was possible to differentiate 23 biotypes of Candida albicans. The biotypes 211, 111 and 811 were most frequently isolated. Most of the samples of C. albicans (98.6 per cent) were sensitive to at least one or more of the nine killer strains. Using the killer system it was possible to show that two patients harbored the same killer biotypes, 111 and 211, respectively, in different clinical specimens and another patient harbored the same biotype (211) in bloodcultures effected in different ocasions. The utilization of the killer system to differentiate types among species of pathogenic yeasts can be a useful method to establish the eventual source of infection, and it is a valuable tool to control and watch for nosocomial infections caused by yeasts.


Sujets)
Humains , Candida albicans/classification , Candida albicans/isolement et purification , Mycotoxines/classification , Protéines fongiques/classification , Techniques de typage mycologique
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