Immunostimulatory activity in mammalian cells of 3, 5-dihydroxy-7-methoxyflavanone ((-)-alpinone) isolated from Heliotropium huascoense resinous exudate / Actividad inmunoestimulante en células de mamífero de 3, 5-dihidroxi-7-metoxiflavanona ((-)-alpinona) aislada del exudado resinoso de Heliotropium huascoense

The flavonoid 3,5-dihydroxy-7-methoxyflavanone ((-)-alpinone) isolated from sticky resinous exudate of Heliotropium huascoense was evaluated as immunostimulatory in mammalian cells . Preliminary observations had showed that (-)-alpinone had increased the expression levels of pro-inflammatory cytokine transcripts in salmonid. Due to high morbidity and mortality that infectious diseases cause in humans, we evaluate the effect of (-)-alpinone as an immunostimulant in mammalian cells. Reactive oxygen species (ROS) are produced by macrophages activators for the destruction of pathogens; we evaluated (-)-alpinone effect in ROS generation and the proliferation of macrophages. The results showed that proliferation in Raw 264.7 cells treated with 10 and 25 µg/mL of (-)-alpinone had a significant increase in macrophage proliferation. In relation to ROS formation, cells treated with 1 and 5 µg/mL of (-)-alpinone, induce ROS formation in macrophages.

El flavonoide 3,5-dihidroxi-7-metoxiflavanona ((-)-alpinona) aislado del exudado resinoso de Heliotropium huascoense se evaluó como inmunoestimulador en células de mamíferos. Resultados preliminares habían demostrado que (-)-alpinona aumentaba los niveles de expresión de transcritos de citoquinas proinflamatorias en salmónidos. Debido a la alta morbilidad y mortalidad que causan las enfermedades infecciosas en los humanos, evaluamos el efecto de (-)-alpinona como inmunoestimulante en células de mamíferos. Dado que las especies de oxígeno reactivo (ROS) son producidas por macrófagos activados para la destrucción de patógenos, se evaluó el efecto de (- )-alpinona en la generación de ROS y la proliferación de macrófagos. Los resultados mostraron que la proliferación en células Raw264.7 tratadas con 10 y 25 µg / mL del flavonoíde tuvo un aumento significativo en la proliferación de macrófagos. En relación con la formación de ROS, las células tratadas con 1 y 5 µg/mL de (-)-alpinona, inducen la formación de ROS en los macrófagos.

Study of the chemical composition of the resinous exudate isolated from Psoralea glandulosa and evaluation of the antioxidant properties of the terpenoids and the resin / Estudio de la composición química del exudado resinoso aislado de Psoralea glandulosa y la evaluación de las propiedades antioxidantes de la resina y sus terpenoides

Otholobium glandulosum (L.) J.W. Grimes (= Psoralea glandulosa L.) (Fabaceae) is a resinous bush that grows in Chile. The chemical composition of its resinous exudate was determined for the first time. Three meroterpenic: bakuchiol (8), 3-hydroxybakuchiol (11), 12-hydroxyisobakuchiol (12) and a new compound kuchiol (13) were isolated and their structures were determined. The antioxidant activity of the terpenic compounds and resin was evaluated using the bleaching of DPPH radical, Ferric Reducing Antioxidant Power (FRAP) and Total Reactive Antioxidant Properties (TRAP) assays.

Otholobium glandulosum (L.) J.W. Grimes (= Psoralea glandulosa L.) (Fabaceae) es un arbusto resinoso que crece en Chile. La composición química de su exudado resinoso fue determinada por primera vez. Tres meroterpenos: bakuchiol (8), 3-hidroxibakuchiol (11), 12-hidroxiisobakuchiol (12) y un nuevo compuesto kuchiol (13) fueron aislados y sus estructuras fueron determinadas espectroscópicamente. La actividad antioxidante de los compuestos terpénicos y la resina se evaluó mediante tres métodos antioxidantes DPPH, Poder Reductor de Hierro III (FRAP) y Capacidad Antioxidante Total (TRAP).

Subcutaneous tissue reaction to castor oil bean and calcium hydroxide in rats

Castor oil bean cement (COB) is a new material that has been used as an endodontic sealer, and is a candidate material for direct pulp capping. OBJECTIVE: The purpose of this study was to evaluate the biocompatibility of a new formulation of COB compared to calcium hydroxide cement (CH) and a control group without any material, in the subcutaneous tissue of rats. MATERIAL AND METHODS: The materials were prepared, packed into polyethylene tubes, and implanted in the rat dorsal subcutaneous tissue. Animals were sacrificed at the 7th and 50th days after implantation. A quantitative analysis of inflammatory cells was performed and data were subjected to ANOVA and Tukey's tests at 5 percent significance level. RESULTS: Comparing the mean number of inflammatory cells between the two experimental groups (COB and CH) and the control group, statistically significant difference (p=0.0001) was observed at 7 and 50 days. There were no significant differences (p=0.111) between tissue reaction to CH (382 inflammatory cells) and COB (330 inflammatory cells) after 7 days. After 50 days, significantly more inflammatory cells (p=0.02) were observed in the CH group (404 inflammatory cells) than in the COB group (177 inflammatory cells). CONCLUSIONS: These results demonstrate that the COB cement induces less inflammatory response within long periods.