Résumé
The present study investigates, by means of a combined enzyme immunoassay for rotaviruses and adenoviruses (EIARA), the occurrence of rotaviruses in stools and jejunal juices from 31 children with acute diarrhea and 18 with chronic diarrhea. Stools from 8 acute cases contained rotaviruses (26%). In two of these cases rotaviruses were also detected in the jejunal juice. In the chronic diarrhea group we identified rotaviruses in the stools of one patient and in the jejunal juice of another. Some of the electropherotypes of the rotaviruses identified showed different patterns of RNA migration. Abnormalities of the jejunal mucosa were characterized in 6 acute rotavirus-positive cases. No morphological or functional abnormalities of the intestinal mucosa were observed in the chornic diarrhea rotavirus-positive cases
Sujets)
Nourrisson , Humains , Mâle , Femelle , Diarrhée du nourrisson/étiologie , Rotavirus/pathogénicité , Électrophorèse sur gel de polyacrylamide , Fèces/microbiologie , Gènes viraux , Techniques immunoenzymatiques , Jéjunum/métabolisme , Rotavirus/génétique , Rotavirus/isolement et purification , Sécrétions intestinales/microbiologieRésumé
Forty normal subjects have been taken for the present study. The mean Indican excretion was 40.45 mg/24 hrs. The mean jejunal count was 1.96 x 10(3) +/- 5.39 x 10(3) organisms/ml and 40% of the jejunal aspirates were sterile. Wide range of bacteria were cultured bu the coliform organisms were obtained in only 16.6%. There was a significant correlation between Indican excretion and total bacterial count (P less than .01).