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Braz. j. microbiol ; 46(2): 443-453, Apr-Jun/2015. tab, graf
Article de Anglais | LILACS | ID: lil-749707

RÉSUMÉ

The diversity of thermophilic bacteria was investigated in four hot springs, three salt marshes and 12 desert sites in Morocco. Two hundred and forty (240) thermophilic bacteria were recovered, identified and characterized. All isolates were Gram positive, rod-shaped, spore forming and halotolerant. Based on BOXA1R-PCR and 16S rRNA gene sequencing, the recovered isolates were dominated by the genus Bacillus (97.5%) represented by B. licheniformis (119), B. aerius (44), B. sonorensis (33), B. subtilis (subsp. spizizenii (2) and subsp. inaquosurum (6)), B. amyloliquefaciens (subsp. amyloliquefaciens (4) and subsp. plantarum (4)), B. tequilensis (3), B. pumilus (3) and Bacillus sp. (19). Only six isolates (2.5%) belonged to the genus Aeribacillus represented by A. pallidus (4) and Aeribacillus sp. (2). In this study, B. aerius and B. tequilensis are described for the first time as thermophilic bacteria. Moreover, 71.25%, 50.41% and 5.41% of total strains exhibited high amylolytic, proteolytic or cellulolytic activity respectively.


Sujet(s)
Bacillaceae/classification , Bacillaceae/isolement et purification , Bâtonnets à Gram positif/classification , Bâtonnets à Gram positif/isolement et purification , Sources thermales/microbiologie , Microbiologie du sol , Microbiologie de l'eau , Biodiversité , Bacillaceae/génétique , Bacillaceae/effets des radiations , Analyse de regroupements , ADN bactérien/composition chimique , ADN bactérien/génétique , ADN ribosomique/composition chimique , ADN ribosomique/génétique , Bâtonnets à Gram positif/génétique , Bâtonnets à Gram positif/effets des radiations , Données de séquences moléculaires , Maroc , Phylogenèse , /génétique , Analyse de séquence d'ADN , Spores bactériens/cytologie
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