Efficacy and safety of oxum in treatment of the venous ulcer.

The present study was carried out to find out the efficacy and safety of oxum in the treatment of venous ulcers. The oxum (superoxidised water) is a pH neutral, non-irritating, aqueous solution that possesses a good antiseptic, antimicrobial activity and wound healing properties. The study was conducted in 30 patients of venous ulcers with a culture examination positive for pathogenic microbial flora. All patients received a gauze dressing impregnated with oxum followed by compression bandage for 28 days. The primary endpoint was the calculation of ulcer size using ulcer tracing. Assessment of periwound oedema, periwound erythema, wound fibrin and wound granulations were considered as secondary endpoints. There was a singificant reduction in ulcer size starting from day 7 of the treatment. Significant improvements in secondary endpoints were observed. This study has demonstrated that oxum improved the clinical status, reduced the signs of inflammation in venous ulcers in addition to its well confirmed anti-infective properties.

Role of nitric oxide and superoxide in Giardia lamblia killing

Giardia lamblia trophozoites were incubated for 2 h with activated murine macrophages, nitric oxide (NO) donors or a superoxide anion generator (20 mU/ml xanthine oxidase plus 1 mM xanthine). Activated macrophages were cytotoxic to Giardia trophozoites (~60 per cent dead trophozoites). This effect was inhibited (>90 per cent) by an NO synthase inhibitor (200 muM) and unaffected by superoxide dismutase (SOD, 300 U/ml). Giardia trophozoites were killed by the NO donors S-nitroso-acetyl-penicillamine(SNAP)and sodium nitroprusside (SNP) in a dose-dependent manner (LD50 300 and 50 muM, respectively). A dual NO-superoxide anion donor, 3-morpholino-sydnonimine hydrochloride (SIN-1), did not have a killing effect in concentration up to 1 mM. However, when SOD (300 U/ml) was added simultaneously with SIN-1 to Giardia, a significant trophozoite-killing effect was observed (~35 per cent dead trophozoites at 1 mM). The mixture of SNAP or SNP with superoxide anion, which yields peroxynitrite, abolished the trophozoite killing induced by NO donors. Authentic peroxynitrite only killed trophozoites at very high concentrations (3 mM). These results indicate that NO accounts for Giardia trophozoite killing and this effect is not mediated by peroxynitrite.

Effect of 24 hour preservation with oxygen free radical scavengers on isolated perfused canine heart lungs

Los efectos de TP-V, una solución hiperosmolar con dextrosa, sucrosa, y ATP-MgCl2 fueron estudiados cuando usada sola o en combinación con substancias que eliminan los radicales libres de oxigenio, por 24 horas de perfusión hipotermica pulsátil de corazón pulmón y después revalorados en un sistema aislado de corazón pulmón a 37-C. Tres grupos de corazón pulmón de perros se analizaron: 1) TP-V (n=6), 2) TP-V con alopurinol (500mg/L) (m=6), 3) TP-V con alopurinol (500mg/L) y catalase (5000U/L) (n=5). Después de 24 horas de preservación, la preparación corazón pulmón fue perfundida con albúmina-manitol por 3 horas a 37-C con objeto de estimar su capacidad funcional y hemodinámica. Grupos 2 y 3 mostraron mejores valores de LDH y CPK a 0 t 3 horas de perfusión. Los valores de pH, pCO2, también como la presión ventricular izquierda, coronaria y aórtica, no cambinaron en forma significante. Histologicamente, el grupo 3 mostró mejor preservación del corazón y pulmones. En resúmen, podemos indicar que la solución TP-V, con las substancias que eliminan radicales libres de oxigeno, ofrecen una buena protección para la preparación corazón pulmón almacenada por 24 horas bajo hipotermia