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1.
Rev. Soc. Bras. Med. Trop ; 51(1): 39-43, Jan.-Feb. 2018. tab
Article Dans Anglais | LILACS | ID: biblio-897046

Résumé

Abstract INTRODUCTION: Rabies is an acute zoonotic disease, caused by a rhabdovirus that can affect all mammals, and is commonly transmitted by the bite of a rabid animal. The definitive diagnosis is laboratorial, by the Fluorescent Antibody Test (FAT) as a quick test and Mouse Inoculation Test (MIT) as a confirmatory test (gold standard). Studies conducted over the past three decades indicate that MIT and Virus Isolation in Cell Culture (VICC) can provide the same effectiveness, the latter being considered superior in bioethics and animal welfare. The aim of this study was to compare VICC with MIT, in terms of accuracy, biosafety and occupational health, supply and equipment costs, bioethics and animal welfare, in a Brazilian public health lab. METHODS: We utilized 400 samples of animal neurological tissue to compare the performance of VICC against MIT. The variables analyzed were accuracy, biosafety and occupational health, time spent in performing the tests, supply and equipment costs, bioethics and animal welfare evaluation. RESULTS: Both VICC and MIT had almost the same accuracy (99.8%), although VICC presented fewer risks regarding biosafety and mental health of the technicians, and reduced time between inoculation and obtaining the results (approximately 22 days less). In addition, VICC presented lower supply costs (86.5% less), equipment costs (32.6% less), and the advantage of not using animals. CONCLUSIONS: These results confirm that VICC can replace MIT, offering the same accuracy and better features regarding cost, results, biosafety and occupational health, and bioethics and animal welfare.


Sujets)
Animaux , Rage (maladie)/diagnostic , Virus de la rage/immunologie , Santé au travail , Technique d'immunofluorescence/méthodes , Techniques de culture cellulaire/méthodes , Confinement de risques biologiques , Questions bioéthiques , Virus de la rage/isolement et purification , Bien-être animal , Reproductibilité des résultats , Facteurs de risque , Technique d'immunofluorescence/économie , Sensibilité et spécificité , Techniques de culture cellulaire/économie , Coûts et analyse des coûts , Souris
2.
Indian J Physiol Pharmacol ; 1998 Apr; 42(2): 155-71
Article Dans Anglais | IMSEAR | ID: sea-108012

Résumé

Getting higher yields of monoclonal antibody (MAb) is a problem in Hybridoma Technology which has two major bottlenecks: a) poor yield of hybridized cells, b) low cellular productivity of MAb in culture. There are three ways of obtaining high MAb yield in vitro a) Large scale culture, b) high density culture and c) enhancing individual cellular productivity in culture. Currently, the focus is on the correct synergistic combination of fortified nutrient media, bioreactor design and mode of operation. Maximization of cell culture longevity, maintenance of high specific antibody secretion rates, nutrient supplementation, waste product minimization and control of environmental conditions are important parameters for improvement of large scale production of MAb. Though, MAb yields have improved rapidly over the decade, there is a growing concern for the decrease in quality of MAb secreted. Further research is therefore necessary to take full advantage of MAb as a potential diagnostic agent for in vivo therapy.


Sujets)
Animaux , Anticorps monoclonaux/biosynthèse , Biotechnologie/économie , Techniques de culture cellulaire/économie , Hybridomes/métabolisme
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