Expression of maspin in benign and malignant salivary gland tumors: An immunohistochemical study.

Context: Maspin is a novel serine protease inhibitor (serpin) with multifaceted tumor‑suppressive activities. It was originally identified in normal human breast myoepithelial cells and shows variable expression in different types of cancer cells. Maspin displays anti‑metastatic properties in mammary and prostate cancer. Its expression is maintained during ovarian, lung and pancreatic carcinogenesis, indicating that Maspin regulated metastatic potential is tissue specific. Thus, it is possible that Maspin participates in salivary gland tumor biology as well. In this study, expression pattern of maspin in benign and malignant salivary gland tumors is analyzed, to understand the biological behavior of salivary gland tumors with respect to maspin expression. Aims and Objectives: The aim of this study was to demonstrate, record, and correlate the expression pattern of maspin in benign and malignant salivary gland tumors. Settings and Design: A retrospective study of maspin expression in 30 diagnosed cases of benign and malignant salivary gland tumors retrieved from archives of our department. Materials and Methods: Anti‑maspin antibody and horseradish peroxidase detection system. Statistical Analysis: Descriptive statistical analysis and Chi‑square/Fisher Exact test. Results: Intense expression with P < 0.001 is associated with benign tumors, nuclear staining with P < 0.001 is significantly associated with benign tumors and cytoplasmic staining with P = 0.020 is associated with malignant tumors. Conclusion: Intensity of expression is more in benign tumors when compared with malignant tumors. The benign tumors showed both nuclear and cytoplasmic expression. Some malignant tumors did express maspin, but mainly in the cytoplasm.

Carcinoma ex Pleomorphic Adenoma of the Salivary Glands: Distinct Clinicopathologic Features and Immunoprofiles Between Subgroups According to Cellular Differentiation

In carcinoma ex pleomorphic adenoma (CXPA), pleomorphic adenoma (PA) and diverse carcinoma components showing luminal (ductal) or non-luminal (myoepithelial) differentiation coexist. To elucidate the clinicopathological implications of cellular differentiation in CXPA and the potential role of p53, vascular endothelial growth factor (VEGF), c-erbB-2, c-kit, and glucose transporter 1 (Glut-1) in carcinogenesis, we analyzed 11 CXPAs with luminal differentiation (CXPAs-LD) and 6 CXPAs with non-luminal differentiation (CXPAs-NLD) and compared protein expressions in residual PAs and carcinomas by immunohistochemistry. Among the CXPAs-LD, 5 were invasive and 8 were histologically high-grade tumors. The 5-year survival rate was 72.7%. P53, c-erbB-2, VEGF, and Glut-1 were more immunoreactive in carcinoma components than in PAs (P = 0.008, 0.004, 0.002, and 0.024, respectively); c-erbB-2 overexpression was associated with high histological grade (P = 0.024). Carcinoma components frequently lacked c-kit expression (P = 0.009). CXPAs-NLD were all low-grade and invasive with a larger mean tumor size (5.2 cm) than CXPAs-LD (3.3 cm) (P = 0.040). The patients remained disease-free without significant immunohistochemical expression. The immunoprofiles and clinical course of CXPA differed according to cellular differentiation. Therefore, it is important to report the histological subtype and to assess potential biomarkers in diagnostic and therapeutic trials.

Expressão das integrinas a2B1, a3B1 e a5B1 em adenoma pleomórfico de glândula salivar menor e carcinoma adenóide cístico / Immunohistochemical expression of a2B1, a3B1 a 5B1 integrins in pleomorphic adenoma from minor major salivary glands and adenoid cystic carcinoma

O adenoma pleomórfico e o carcinoma adenóide (CAC) representam neoplasias de glândula salivar e maligna, respectivamente, as quais compartilham algumas características com a mesma origem celular e uma marcante presença de matriz extracelular, apresentando, porém, comportamento biológicos distintos. O propósito desta pesquisa consistiu em comparar a expressão das integrinas a2B1, a3B1, e a5B1 em adenomas pleomórficos de glândula salivar menor e maior e CACs. Além disso, procurou investigar se havia diferenças na expressão destas integrinas entre os subtipos histológicos do CAC. Foram selecionados 14 casos de adenomas pleomórfico de glândula salivar maior, 14 casos de glândula salivar menor e 10 casos de CACs. Analisou-se a presença ou ausência, localização e intensidade de marcação das integrinas. Os dois grupos de adenomas pleomórfico foram reunidos em um só para fazer a comparação entre os dois tumores. Verificou-se que houve diferença estatística altamente significativa (p<0,0001) para a integrina a2B1 entre os dois tumores, apresentando o adenoma pleomórfico, uma marcação mais intensa para esta integrina. Em relação à integrina a5B1 não foi possível a realização de testes estatísticos, ficando patente, porém, que houve uma tendência da referida integrina ser mais intensamente expressa no adenoma pleomórfico. Para a ánalise comparativa, os CACs foram subdivididos em 2 grupos: sólido e tubular/cribriforme. Para a integrina a2B1 observou-se que não houve diferença estatisticamente significativa e em relação à a3B1 e a5B1 não foi possível a realização do teste estatístico; no entanto, também foi verificada uma clara tendência para os casos do subtipo sólido apresentarem expressão ausente ou reduzida das integrinas avaliadas

Aparición de componente monoclonal en Síndrome de Sjögren: estado pre-linfomatoso / Detection of a monoclonal component in Sjögren syndrome: prelymphomatous stage

Se estudian dos casos de Síndrome de Sjögren (SS) que se asocian al componente monoclonal (IgG Kappa y Lambda) describiendo especialmente la presentación con vasculitis a repetición del primero y el agrandamiento de glándulas salivales en el segundo. Ambos no cubren criterios para enfermedad alguna del colágeno ni se confirma la existencia de proceso linfoproliferativo maligno hasta la fecha. Se delimitan dos subsets en SS con distinta orientación evolutiva y se discute el significado de la aparición en sangre periférica de una paraproteína como estado pre-linfomatoso a veces de duración indefinida, siendo considerada la glándula salivar el primer sitio de transformación maligna