Your browser doesn't support javascript.
loading
Montrer: 20 | 50 | 100
Résultats 1 - 8 de 8
Filtre
1.
Arq. neuropsiquiatr ; 73(7): 561-568, 07/2015. tab, graf
Article Dans Anglais | LILACS | ID: lil-752384

Résumé

We studied 36 glioblastoma cases at HC-UNICAMP from 2008 to 2012 and classified the immunohistochemical distribution of the wild-type epidermal growth factor receptor (EGFR), mutated forms of p53 protein and isocitrate dehydrogenase-1 (IDH-1) and murine double protein 2 (MDM2). Immunostaining findings were correlated with clinical data and response to treatment (surgery, chemotherapy and radiotherapy). About 97% of the tumors were primary, most of them localized in the frontal lobe. Mean time free of clinical or symptomatic disease and free time of radiological disease were 7.56 and 7.14 months, respectively. We observed a significant positive correlation between expressions of p53 and MDM2, EGFR and MDM2. Clinical, radiological and overall survivals also showed a significant positive correlation. p53 staining and clinical survival showed a significant negative correlation. The current series provides clinical and histopathological data that contribute to knowledge on glioblastoma in Brazilians.


Estudamos 36 casos de glioblastoma acompanhados no HC-UNICAMP de 2008 a 2012 e classificamos a marcação imunoistoquímica da forma selvagem do receptor do fator de crescimento epidérmico (EGFR), formas mutantes da proteína p53 e isocitrato desidrogenase-1 (IDH-1) e proteína murina dupla 2 (MDM2). Os resultados de imunoistoquímica foram correlacionados com dados clínicos e resposta ao tratamento (cirurgia, quimioterapia e radioterapia). Cerca de 97% dos tumores foram primários, grande parte localizada no lobo frontal. O tempo médio livre de doença clínica ou sintomática e o tempo livre de doença radiológica foram de 7.56 e 7.14 meses, respectivamente. Observou-se correlação positiva entre a expressão das proteínas p53 e MDM2, EGFR e MDM2. Sobrevivências clínica, radiológica e global também mostraram correlação positiva e significativa. A expressão para p53 e sobrevivência clínica mostrou correlação negativa. O estudo fornece dados clínicos e histopatológicos que contribuem para o conhecimento sobre glioblastoma em brasileiros.


Sujets)
Adulte , Sujet âgé , Sujet âgé de 80 ans ou plus , Femelle , Humains , Mâle , Adulte d'âge moyen , Jeune adulte , Marqueurs biologiques tumoraux/analyse , Tumeurs du cerveau/composition chimique , Glioblastome/composition chimique , Isocitrate dehydrogenases/analyse , /analyse , Récepteurs ErbB/analyse , /analyse , Marqueurs biologiques tumoraux/génétique , Tumeurs du cerveau/mortalité , Tumeurs du cerveau/anatomopathologie , Tumeurs du cerveau/thérapie , Glioblastome/mortalité , Glioblastome/anatomopathologie , Glioblastome/thérapie , Immunohistochimie , Isocitrate dehydrogenases/génétique , Mutation , Pronostic , /génétique , Valeurs de référence , Études rétrospectives , Récepteurs ErbB/génétique , Statistique non paramétrique , /génétique
2.
Arq. neuropsiquiatr ; 69(1): 112-117, Feb. 2011. ilus, graf
Article Dans Anglais | LILACS | ID: lil-598356

Résumé

Several animal experimental models have been used in the study of malignant gliomas. The objective of the study was to test the efficacy of a simple, reproducible and low cost animal model, using human cells of glioblastoma multiforme (GBM) xenotransplantated in subcutaneous tissue of Wistar rats, immunosuppressed with cyclosporin given by orogastric administration, controlled by nonimunosuppressed rats. The animals were sacrificed at weekly intervals and we have observed gradual growth of tumor in the immunosuppressed group. The average tumor volume throughout the experiment was 4.38 cm³ in the immunosuppressed group, and 0.27 cm³ in the control one (p<0.001). Tumors showed histopathological hallmarks of GBM and retained its glial identity verified by GFAP and vimentin immunoreaction. Immunosuppression of rats with cyclosporin was efficient in allowing the development of human glioblastoma cells in subcutaneous tissues. The model has demonstrated the maintenance of most of the histopathological characteristics of human glioblastoma in an heterotopic site and might by considered in research of molecular and proliferative pathways of malignant gliomas.


Vários modelos animais têm sido avaliados no estudo dos gliomas e até o momento nenhum pôde ser considerado ideal. O objetivo deste trabalho é verificar a eficácia de um modelo animal simples, reprodutível e de baixo custo. Utilizamos células humanas de glioblastoma multiforme (GBM) xenotransplantadas em ratos Wistar, submetidos a imunossupressão com ciclosporina administrada por via orogástrica. Células tumorais foram implantadas no tecido subcutâneo dos ratos imunossuprimidos com ciclosporina, sendo o controle feito em ratos não imunossuprimidos. Os animais foram sacrificados em intervalos semanais e foi observado crescimento progressivo do tumor no grupo imunossuprimido. A média do volume tumoral em todo o experimento foi de 4,38 cm³ no grupo imunossuprimido e 0,27 cm³ no grupo controle (p<0,001). Os tumores apresentavam características histopatológica do GBM e mantinham sua identidade glial, verificadas por imunoreação para GFAP e vimentina. A imunossupressão dos ratos com ciclosporina foi eficiente em permitir o desenvolvimento do glioblastoma no tecido subcutâneo. Uma vez que o presente modelo mantém a maioria das características histopatológicas do glioblastoma humano, ele pode ser considerado em estudos que avaliem as vias moleculares e proliferativas dos gliomas malignos.


Sujets)
Animaux , Humains , Mâle , Rats , Tumeurs du cerveau/anatomopathologie , Ciclosporine/administration et posologie , Glioblastome/anatomopathologie , Immunosuppresseurs/administration et posologie , Transplantation tumorale/méthodes , Administration par voie orale , Tumeurs du cerveau/composition chimique , Glioblastome/composition chimique , Modèles animaux , Protéines tumorales/analyse , Protéines de tissu nerveux/analyse , Rat Wistar , Charge tumorale , Transplantation hétérologue/méthodes , Vimentine/analyse
3.
Indian J Pathol Microbiol ; 2007 Oct; 50(4): 754-8
Article Dans Anglais | IMSEAR | ID: sea-75826

Résumé

The accurate grading of astrocytic tumours is of prime importance because it is critical to the patient management and survival/outcome. Although internationally accepted WHO grading system of CNS tumours is based on histological features of H&E stained sections, yet there are cases where differentiation between grade II and grade III is difficult particularly when the biopsy is small. Proliferative index derived from MIB-1 immunostaining has been found to be useful in the distinction between various grades of malignancy. Formalin-fixed paraffin-embedded surgical specimens from 90 cases of astrocytic tumours, 30 each of low-grade astrocytoma (grade II), anaplastic astrocytoma (grade III), and glioblastoma multiforme (grade IV), were immunostained by standard indirect immunoperoxidase technique using MIB-1 monoclonal antibody. MIB-1 labeling index (MIB-1 LI) was calculated. The mean MIB-1 LI values of astrocytomas, anaplastic astrocytomas and glioblastomas were 1.75 +/- 1.5%, 8.74 +/- 6.2%, and 20.54 +/- 12.2% respectively and there was statistically significant difference between grade II and III (Unpaired "t" test, T value 5.907, p value < 0.001) and grade III and grade IV (T value 4.734, p value < 0.001). The statistical analysis also revealed that the mean MIB-1 LI increased with histological grade of malignancy (One way ANOVA test, p value < 0.001). This investigation further reinforces and corroborates the findings that MIB-1 LI is useful tool in assigning grading to the astrocytic tumours and hence in treatment modalities and should be used routinely.


Sujets)
Astrocytome/composition chimique , Tumeurs du cerveau/composition chimique , Prolifération cellulaire , Flavivirus , Glioblastome/composition chimique , Humains , Immunohistochimie/méthodes , Indice de gravité de la maladie , Ubiquitin-protein ligases/analyse
4.
J Cancer Res Ther ; 2007 Jan-Mar; 3(1): 47-9
Article Dans Anglais | IMSEAR | ID: sea-111530

Résumé

During a mitral valve replacement surgery in a girl of 22 years of age, it was accidentally discovered that the valve was destroyed due to a tumor and the histopathology and immunohistochemistry findings have proved it to be undifferentiated sarcoma. She was advised by the surgeon to go for chemotherapy. There was a delay of three months from the side of the patient to reach us and during that interval she has developed secondaries in the brain. This case is being presented here for its rarity.


Sujets)
Adulte , Tumeurs du cerveau/composition chimique , Association thérapeutique , Échocardiographie , Femelle , Tumeurs du coeur/composition chimique , Implantation de valve prothétique cardiaque , Humains , Valve atrioventriculaire gauche , Sarcomes/composition chimique , Résultat thérapeutique , Marqueurs biologiques tumoraux/analyse
6.
Article Dans Anglais | IMSEAR | ID: sea-40917

Résumé

Desmoplastic infantile ganglioglioma (DIG) is an uncommon neuroepithelial tumor associated with epilepsy, mostly occurring in the first 2 years of life. Most DIGs carry good prognosis after complete resection, even when a primitive cellular element is present. However a few examples of DIG with histologic anaplasia have recently been reported, and one demonstrated an unusual aggressive behavior. The authors describe herein a DIG with high Ki-67 proliferation index (30%) in a 10-month-old male infant with epilepsy, but with an excellent prognosis after total tumor resection.


Sujets)
Tumeurs du cerveau/composition chimique , Prolifération cellulaire , Gangliogliome/composition chimique , Humains , Nourrisson , Antigène KI-67/analyse , Mâle
7.
Neurol India ; 2004 Jun; 52(2): 228-32
Article Dans Anglais | IMSEAR | ID: sea-121338

Résumé

BACKGROUND: p53 is a tumor suppressor gene implicated in the genesis of a variety of malignancies including brain tumors. Overexpression of the p53 protein is often used as a surrogate indicator of alterations in the p53 gene. AIMS: In this study, data is presented on p53 protein expression in adult cases (>15 years of age) of astrocytic (n=152) and oligodendroglial (n=28) tumors of all grades. Of the astrocytic tumors, 86% were supratentorial in location while remaining 14% were located infratentorially - 8 in the the cerebellum and 13 in the brainstem. All the oligodendrogliomas were supratentorial. MATERIALS AND METHODS: p53 protein expression was evaluated on formalin-fixed paraffin-embedded sections using streptavidin biotin immunoperoxidase technique after high temperature antigen retrieval. RESULTS: Overall 52% of supratentorial astrocytic tumors showed p53 immunopositivity with no correlation to the histological grade. Thus, 58.8% of diffuse astrocytomas (WHO Grade II), 53.8% of anaplastic astrocytomas (WHO Grade III) and 50% of glioblastomas (WHO Grade IV) were p53 protein positive. In contrast, all the infratentorial tumors were p53 negative except for one brainstem glioblastoma. Similarly, pilocytic astrocytomas were uniformly p53 negative irrespective of the location. Among oligodendroglial tumors, the overall frequency of p53 immunopositivity was lower (only 28%), though a trend of positive correlation with the tumor grade was noted - 25% in Grade II and 31.5% in grade III (anaplastic oligodendroglioma). Interestingly, p53 labeling index (p53 LI) did not correlate with the histopathological grade in both astrocytic and oligodendroglial tumors. CONCLUSIONS: Thus, this study gives an insight into the genetic and hence biological heterogeneity of gliomas, not only between astrocytic tumors vs. oligodendrogliomas but also within astrocytic tumors with regard to their grade and location. With p53 gene therapy trials in progress, this will possibly have future therapeutic implications.


Sujets)
Adolescent , Adulte , Sujet âgé , Astrocytome/composition chimique , Tumeurs du cerveau/composition chimique , Humains , Immunohistochimie , Tumeurs sous-tentorielles/composition chimique , Adulte d'âge moyen , Oligodendrogliome/composition chimique , Tumeurs sus-tentorielles/composition chimique , Protéine p53 suppresseur de tumeur/analyse
8.
Journal of Korean Medical Science ; : 555-559, 2000.
Article Dans Anglais | WPRIM | ID: wpr-150734

Résumé

Recent evidence shows that transcriptional silencing as a consequence of hypermethylation of CpG islands is an important mechanism in the inactivation of p16INK4 tumor suppressor gene. This study is designed to clarify the significance of p16INK4 hypermethylation in 23 cases of glioblastomas (GBMs) by methylation-specific polymerase chain reaction (PCR) and p16 immunostaining. Fourteen cases (60.9%) out of 23 GBMs revealed hypermethylation on p16. p16 immunostaining revealed that 13 (93%) of these 14 hypermethylation cases showed complete loss of immunoreactivity and only one (7%) case retained immunoreactivity. Among 9 methylation-negative cases, 4 were immunonegative, which might be related to mutations or deletions other than hypermethylation. The most significant finding was that of 17 cases with immunonegativity, 13 cases (76.5%) showed hypermethylation. We reconfirmed that p16 hypermethylation may be one of the major mechanisms of tumorigenesis of GBMs and the results between the methylation specific-PCR study and p16 immunostaining had a good correlation.


Sujets)
Adulte , Femelle , Humains , Mâle , Régions 5' non traduites/métabolisme , Régions 5' non traduites/génétique , Éléments antisens (génétique) , Tumeurs du cerveau/anatomopathologie , Tumeurs du cerveau/génétique , Tumeurs du cerveau/composition chimique , Ilots CpG/physiologie , Méthylation de l'ADN , Extinction de l'expression des gènes/physiologie , Glioblastome/anatomopathologie , Glioblastome/génétique , Glioblastome/composition chimique , Adulte d'âge moyen , Réaction de polymérisation en chaîne , Inhibiteur p16 de kinase cycline-dépendante/génétique , Inhibiteur p16 de kinase cycline-dépendante/analyse
SÉLECTION CITATIONS
Détails de la recherche