Résumé
ABSTRACT This communication reports full genome sequencing of the bluetongue virus-1 (BTV-1) isolate MKD20/08/Ind from goat in northern India. The total BTV-1 genome size was found to be 19,190 bp. A comparison study between the Indian isolate and other global isolates revealed that it belongs to the 'Eastern' BTV topotype. The full genome sequence of BTV-1 will provide vital information on its geographical origin and it will also be proved useful for comparing the Indian isolate with global isolates from other host species.
Sujets)
Animaux , Capra/virologie , Génome viral , Analyse de séquence d'ADN , Virus de la langue bleue/génétique , Phylogenèse , Virus de la langue bleue/isolement et purification , Virus de la langue bleue/classification , Génomique , Séquençage nucléotidique à haut débit , Sérogroupe , IndeRésumé
Bluetongue (BT) is a viral disease of domestic and wild ruminants. It is particularly damaging in sheep, where up to half of infected animals may die, showing inflammation and hemorrhages of the mucous membranes of the mouth, nose, and intestines. In cattle and goats, BT rarely causes disease, however it can affect the animal's reproductive ability, so that losses are not easily estimated. Bluetongue virus spreads from animal to animal by biting insects of the genus Culicoides; and this is the reason why the disease is more prevalent in geographic areas where climate conditions are favourable for their development. The disease was first recognized in South Africa in the late 1700's, but it was not until the early 1900's that it was described in detail, and at present, epizootiology and pathogenesis studies are still being carried on.
Sujets)
Animaux , Mâle , Femelle , Fièvre catarrhale du mouton , Virus de la langue bleue , Avortement chez les animaux , Antigènes viraux/immunologie , Argentine , Fièvre catarrhale du mouton , Ceratopogonidae , Maladies foetales/médecine vétérinaire , Maladies foetales/virologie , Infertilité masculine , Vecteurs insectes , Protéines virales/immunologie , ARN viral , Ruminants , Vaccins antiviraux , Virus de la langue bleue/classification , Virus de la langue bleue/isolement et purification , Virus de la langue bleue/physiologieRésumé
Bluetongue virus serotype 1 (Avikanagar isolate) was grown in BHK-21 cell line and titrated. The titre of the virus in BHK-21 cell line was 10(6) TCID50/ml. RNA-polyacrylamide gel electrophoresis (RNA-PAGE) and dot immunobinding assay (DIA) were performed on 10-fold serial dilutions of the sonicated cell culture material. The results indicated that the minimum limit of detection of the virus by RNA-PAGE and DIA was 10(5) TCID50/ml.