Résumé
Abstract Considering oral diseases, antibiofilm compounds can decrease the accumulation of pathogenic species such as Streptococcus mutans at micro-areas of teeth, dental restorations or implant-supported prostheses. Objective To assess the effect of thirteen different novel lactam-based compounds on the inhibition of S. mutans biofilm formation. Material and methods We synthesized compounds based on γ-lactones analogues from rubrolides by a mucochloric acid process and converted them into their corresponding γ-hydroxy-γ-lactams by a reaction with isobutylamine and propylamine. Compounds concentrations ranging from 0.17 up to 87.5 μg mL-1 were tested against S. mutans. We diluted the exponential cultures in TSB and incubated them (37°C) in the presence of different γ-lactones or γ-lactams dilutions. Afterwards, we measured the planktonic growth by optical density at 630 nm and therefore assessed the biofilm density by the crystal violet staining method. Results Twelve compounds were active against biofilm formation, showing no effect on bacterial viability. Only one compound was inactive against both planktonic and biofilm growth. The highest biofilm inhibition (inhibition rate above 60%) was obtained for two compounds while three other compounds revealed an inhibition rate above 40%. Conclusions Twelve of the thirteen compounds revealed effective inhibition of S. mutans biofilm formation, with eight of them showing a specific antibiofilm effect.
Sujets)
Streptococcus mutans/effets des médicaments et des substances chimiques , Biofilms/effets des médicaments et des substances chimiques , bêta-Lactames/pharmacologie , Lactones/pharmacologie , Antibactériens/pharmacologie , Plancton/croissance et développement , Plancton/effets des médicaments et des substances chimiques , Streptococcus mutans/croissance et développement , Microscopie électronique à balayage , Numération de colonies microbiennes , Tests de sensibilité microbienne , Reproductibilité des résultats , Analyse de variance , Biofilms/croissance et développement , Résistance aux bêta-lactamines/effets des médicaments et des substances chimiques , bêta-Lactames/synthèse chimique , Relation dose-effet des médicaments , Viabilité microbienne/effets des médicaments et des substances chimiques , Chlorure de méthylrosanilinium , Lactones/synthèse chimique , Antibactériens/synthèse chimiqueRésumé
Two Mg [II] complexes have been synthesized by condensing magnesium chloride with schiff bases of two different beta-lactams. In the present study, cephalexin and cefadroxil were the beta-lactams used for the synthesis of complexcs through schiff base formation. The estimation of carbon, hydrogen. nitrogen and sulphur, percentage of magnesium, and measurcments of IR spectra at 400- 4000cm[-1] indicated the formation of coordination compounds. The ratio of 2:1 complex of schiff base ligand to metal was confirmed through Job's of continuous variation. Further characterization of the complexes was carried out using UV spectra, mass spectra and conductance measurement