Ileocystoplasty in dogs: Imaging, hematologic, biochemical and histopathologic studies

The radiographic, ultrasonographic, hamatologic, biochemical and histopathologic studies were done the following routine ileocystoplasty in dogs. Experimental study. Twelve female mixed-breed Persian dogs. Persian mixed breed female dogs between 1-2 year-old and 15-24 kg body-weight were divided into control and experiment groups. Routine ileocystoplasty was performed on experimental dogs. Twenty centimeters of ileal segments were detubulized to U-form and were grafted to bladder after performing the partial supratrigonal cystectomy. Radiography, ultrasonography, hematologic and biochemical evaluations were prepared on days 0,14 and 35 from all animals. Experimental group animals also were euthanized on day 35. Neobladders were resected and histopathologic sections were taken. No radiographic changes were detected in plain and contrast radiography indicative of any leakage from anastomotic and suture lines. Vesicoureteral reflux,... was not present. Ultrasonography revealed neobladder walls thickened at the anastomotic site. No significant changes were found in hematologic parameters, glucose, cholestrol, triglyceride, creatinine, total protein, albumine, glutamine, serum electrolytes [calcium, potassium, phosphorus, sodium, chloride and bicarbonate] albumin, pH, venous blood gases [Po2 and Pco2] and anion gap. BUN was found significantly higher in experiment group in comparison to control group.There were not marked morphologic changes in ileal mucosa, uroepithelial migrated from remaining portion of the bladder to cover the ileal segments. Inflammatory cells infiltrations were not significant in neobladders. Based on the study, ileal segment is useful source for graft the bladder in dog

[Clinical evaluation of ileocystoplasty in dogs]

To evaluate the clinical features of ileocystoplasty [bladder augmentation] in dogs.Experimental study. Seven female 1-2 year-old mixed breed dogs between 15- 24 kg body-weight. Routine ileocystoplasty [similar to human ileocystoplasty] was performed on dogs. The clinical parameters [temperature, heart rate per minute, respiratory rate per minute, urine appearance, urinary continence, urethral obstruction following mucosal secretion and bowel obstruction] were evaluated for five weeks. A repeated measures [within-subjects] analysis of variance was done to compare the means of quantitative variables at different times and descriptive study for qualitative variables. Dogs tolerated the operation well and survived for 5 weeks without any postoperative complications. There no significant difference in heart rate per minute and respiratory rate per minute in the study period. A significant increase in temperature was observed at day 28 due to other laboratory examinations, but it was in normal range [P<0.05]. No abnormal findings were found in appetite, defecation and its quality, hydration status, mucosal color, capillary refill time, urine appearance, urine continence, urethral obstruction following mucosal secretion and bowel obstruction during study.With regards to the clinical observations in this study ileocystoplasty could be favorably performed in bladder disorders e.g. cancer, trauma, infection, inflammation, iatrogenic injuries or conditions which may lead to severe dysfunction in dogs

[Determination of the proper time to de-epithelialization of ileal segment by using the enzyme composition [cocktail] in dog]

Objective: to determine of a proper time to de-epithelialization of ileal segment by using collagenase and trypsin cocktail for ileocystoplasty

Design: descriptive experimental study

Animals: fifteen adult female mixed Persian dogs

Procedure: ileocystoplasty was performed on 15 female Persian mixed breed dogs ranging from 2 to 3 years old and 15 to 24 kg body weight. Twenty centimeters of ileal segment with its adequate mesentery was selected. Five millimeters of ileal segment was taken for normal histological study of the intestine. Enzymatic treatment of ileal segment using 0.125% collagenase and trypsin cocktail in 5, 10, 15, 20 and 25 minutes was performed on dogs. After each enzymatic exposure, tissue samples [5 mm] were taken for histopathologic evaluation

Results: microscopical study of 180 histologic sections showed that 5 minutes treatment [MT], focally denuded 20% of villi epithelium; while 10 MT has caused 33% of villi epithelium focally. In 15 MT approximately 60% of villi were de-epithelialized while 20 MT caused 75% villi de-nudation with more cryptlysis. Complete de-nudation of villi epithelium occurred with 25 MT

Clinical implications: the results of this preliminary study state reveal that approximately 25 minutes enzymatic treatment of ileal segment can completely de-epithelialize villi, and is recommended for ileocystoplasty