[Evaluation of chimerism in interphase nuclei of peripheral blood of patients with sex-mismatch bone marrow transplantation]

Fully quantitative cytological techniques for the analysis of hematopoietic chimerism are very limited and are largely restricted to sex - chromosome detection after sex - mismatched bone marrow transplants [BMTs]. The aim of the present investigation was to evaluate the application of FISH technique in peripheral blood interphase nuclei of three patients who had received sex - mismatched bone marrow transplants. The FISH technique shows 40% to 85% [average 61%] chimerism in these patients. All the three studied patients having undergone bone marrow transplants have achieved complete remission now. Evaluation of mixed chimerism has potential applications in controlling self-reactions avoiding rejection of the transplant