RESUMO
Rofecoxib [selective cycIo-oxygenase-2-inhibitor] had a wide range of surgical therapies especially [analgesic] in the peri-operative period. Its effects on the intestinal wound healing is unknown. The aim of the studied was to investigate effects of Rofecoxib on colonic anastomosis in rats in the first post-operative week. The study included 40 male rats divided into two groups, studied, 20 and control 20. In both, resection-anastomosis of left colon with interrupted single layer of vicryl 6/0 was done. The control group received distilled water and the studied group received Rofecoxib pre-operatively and during the first post-operative week. Bursting pressure of the anastomosis in the studied group decreased from 116.40 + 10.20mmHg preoperatively to 41.60 + 3.00 mmHg and 49.40 + 5.79 mmHg respectively at 3 and 7 days post-operatively [P<0.001] compared to 124.05 + 11.15 mmHg, 107.70 + 12.90 mmHg and 114.55 + 12.91 mmHg respectively in the control group at the same intervals. Epithelialization 0f grade I with numerous inflammatory cells was noted in the anastomosis in the studied group at the third post-operative day, while epithelialization of grade III and less numerous inflammatory cells together with reformed lamina propria and congested blood vessels were noted at the seventh post-operative day. Incidence of colonic of fistula was 33% in the studied group versus 9% in the control group at the seventh post-operative day While mortality was 25% in the studied group compared to 9% in the control group at the seventh post-operative day. No fistula or mortality occurred on the third post-operative day. In conclusion, administration of Rofecoxib significantly impaired healing [decreased cellular proliferation and collage deposition] and decreased bursting pressure of colonic anastomosis in rats during the first post-operative week. Rofecoxib increased incidence of fistula in this period
RESUMO
A new model for breast cancer is needed to define the fine dynamic balance between the tumor and the host including various autocrine and endocrine factors which influence proliferation, apoptosis and angiogenesis. Aim of the study was to measure the systemic and local anti-tumor immune response for breast cancer, to study cytokine network modification [IL-10] [anti-inflammatory] and IL-12 [pro-inflammatory] and to evaluate its correlations with other histopathological parameters. The study was done on 17 female patients with breast cancer and another control group of 10 patients. The patients were followed-up for two years. Significant correlations of studied peripheral blood immune parameters were: Natural Killer [NK] numbers, NK activity [NKA] and peripheral T-lymphocytes in count per minute [0pm] with tissue NK [TNK] and tissue natural Killer Activity [NKA]. Significant correlation between NKA and IL-12, and peripheral blood T-lymphocytes with NK and tissue NK activity [T-NKA] was detected. Significant correlations between IL-10, IL-12 and the other parameters were detected. Significant correlation was noticed between [Local immune response]: tumor infiltrating lymphocytes [171.3] and tumor Stage, also between other studied parameters of local immune response. The degree of fibrosis [mechanical tumor control] was correlated negatively with the tumor grade and lymph node number. The disease free survival [D.F.S] was significantly related to tissue local immune response and systemic immune response through IL-12 modifications. Significant correlation between high levels of IL-10 with NKA and TILs was detected Recurrence was closely related to the number of positive axillary lymph nodes. On conclusion: proper function of peripheral T-lymphocytes was crucial for effective destruction of breast cancer cells. NKA Was involved in breast cancer disease progression and it depended on levels of IL-12 and the antigen presenting cell [APC]. IL-10 had a predictive role in breast cancer response and exogenous anti-IL-10 may be useful. The amount of IL-12 available was critical for tumor progression. TIL: correlates with the tumor stage but failure of tumor eradication may be due to higher levels of IL-10. The mechanical arm was involved in the immune response disease. Free Survival [DIES] was related to KA and SNKA through the lL-12. Lastly the discriminate factor in DFT was the TNKA