[Chemical composition and anti-candida activity of Trachyspermum ammi essential oil on azoles resistant Candida albicans isolates from oral cavity of HIV+ patients]

Oral candidiasis, caused by Candida albicans, is one of the most common infections in immunocompromised patients, especially in HIV+ individuals. The aims of this study were to evaluate the susceptibility of C. albicans isolates to azole drugs and Trachyspermum ammi essential oil. Oral swabs were cultured from 70 HIV+ patients and In order to identify and confirm of C. albicans isolates, Chrom agar, Corn meal agar, germ tube production, carbohydrate assimilation, growth at 45[degree sign]C and PCR were performed. Sensivity to fluconazol, ketoconazole and clotrimazol were assessed by disc diffusion and also the effect of T. ammi essential oil was determined by disc diffusion and microdilution broth methods. The causative agent, in 50 patients with oral candidiasis, was C. albicans [71.4%]. In sensivity determination survey to antifungal drugs, the resistance of isolates to fluconazole, ketoconazole and clotrimazole were determined 32%, 28% and 14%, respectively. In disc diffusion, all isolates have an acceptable sensivity at 10 - 20 micro L of the oil and 30 micro L inhibit the growth completely in plate. Minimum Inhibitory Concentrsation [MIC] by microdilution broth method was 500ppm and 750ppm in 72% and 28% of isolates, respectively, and Minimum Fungicidal Concentration[MFC] in 70% of isolates were 750ppm and for the rest of the isolates [30%] were 1000ppm. We conclude that use of this native plant, as an antifungal compound, could act as a treatment of the patients with mucosal candidiasis, beside of other drugs in to the future

[ study on the hepatic abscess of slaughtered cattl in shahrekord abattoir]

To determine occurrence and also bacterialcauses of hepatic abscess of sacrificed cattle in shahrekodabattoir.Cross Sectional study.Five hundreds sacrificed cattle.During this study inspection of 500 sacrificedcattle for presence of hepatic abscess was carried out inslaughter house. After observing the hepatic abscess, animal's sex, age, production, pregnancy and also abscesscharacterizations [number, size, location], recorded andwhole abscess was dissected from the liver and transferredto the laboratory. Aerobic, anaerobic and microaerophilicbacterial cultures from hepatic abscesses were carried outusing standard methods.Chi- Square statistical test.Thirty three cattle [6.6%] out of 500 inspectedsacrificed cattle had hepatic abscess, from which 18abscesses were in females [54.5%] and 15 abscesses werein male [45.5%]. Twenty three livers out of 33 infectedlivers had only one abscess [69.69%] and 10 livers had 2 ormore abscesses [30.30%]. Fusobacterium necrophorumwas isolated as unique bacterial cause of 21 hepaticabscesses [63.63%]andArcanobacteriumpyogenes wasisolated from only 9 abscesses [27.27%].The results of the present study showed that Rnecrophorum is the most important bacterial cause ofhepatic abscesses in cattle in shahrekord. FurthermoneArcanobacterium pyogenes isthesecond importantbacterial cause of hepatic abscesses