Embryo production in buffaloes [Bubalus bubalis] with some supplementation in superovulatory regimens

The aim of this study is to evaluate the effect of different superovulatory regimens on embryo production of buffalo-cows in Egypt. Estrus synchronization applied on 16 multiparous Egyptian buffalo-cows divided randomize into three groups A [n=4], B [n=6] and C [n=6]. Only, thirteen buffalo cows responded in the three groups A [n=3]; B [n=4] and C [n=6]. The buffaloes showed estrus from the three groups were received a dose of 2500 IU of eCG on day 8 of the cycle. In group A [n=3] no, further superovulatory treatment was applied, whereas animals of group B [n=4] received a single dose of 2500 IU of hCG 30 h after AI. Buffaloes of group C [n=6] received the same regimen of group B in addition to i.m injection of 2.5 mg Estradiol benzoate [EB] and 50 mg progesterone [P4] 4 days before superovulatory treatments. Embryos were collected from all groups non-surgically during day 6 [around 140] h from estrus onset. The results reveals that there were a significance increase [P<0.05] for the number of corporalutea and collected embryos in group C than other treated groups. In conclusion, progesterone supplementation with estradiol benzoate 4 days before initiating the superovulation treatment seems to enhance the recovery rate in buffaloes. Further studies are needed in order to increase the number of embryos recovered per a buffalo female

Effect of type of insemination on embryonic mortality and stillbirth in dairy cattle and heifers

The aim of this work was to evaluate the rate of embryonic mortality in early pregnancy period and rate of stillbirths at calving period in naturally and artificially inseminated dairy cattle and heifers. The study was carried out in October, 2007-November, 2008 in a cattle farm located at Alex desert road, El Behera province. Animals divided into two groups: The first one naturally inseminated [20 cows and 8 heifers]. Group two [34 cows and 12 heifers] were artificially inseminated. Pregnancy diagnosis was carried out by ultrasonography on days 28 and 35 then, confirmed rectally on day 45 post insemination. Animals that were pregnant on day 28 but not on day 35 were considered to have undergone embryonic mortality [EM]. The rate of embryonic mortality in Heifers those naturally inseminated had significant variance [p<0.05] from day 35 [0.0] to day 45[16.67%] post insemination rather than artificial insemination on day 35 [12.50%] to day 45[14.29%]. While, cows those naturally inseminated had significant variance [p<0.05] from day 35 [7.69%] to day 45[16.67%] post insemination rather than artificial insemination on day 35 [4.35%] to day 45[4.55%]. Stillbirth in calves without regarding to the type of animals [Cows or Heifers] had significant increase [p<0.05] with natural insemination [33.33%] rather than artificial insemination [22.22%]. In conclusion, the use of AI needs to be mandatory strategies in order to obtain high fertilization rates and maintenance of pregnancy in cattle and consequently lead to reduction of dystocia and improvements in calf health and survival. Future studies should attempt to biotechnologies of AI techniques as well as other traits that contribute to lifetime profitability, because precise knowledge of such parameters is needed to develop efficient and effective dairy crossbreeding systems

Effect of the dose of equine chorionic gonadotrophin [ECG] on ovarian response and embryo recovery in Egyptian baladi goats

The effects of doses of equine chorionic gonadotrophin [eCG] for a simple superovulation method in does [Goats] were investigated on ovulation rate, proportions of fertilized oocytes and normal embryos at recovery. The does were treated with intravaginal sponges impregnated with medroxy progesterone acetate [MAP] for 12 days. For superovulation, an intramuscular injection of eCG was given at 24 h before sponge removal. Does were divided into nine equal groups [n = 5] A, B, C, D, E, F, G, H and I. Each received 100, 200, 300, 400, 500, 600, 700, 800 and 900 IU of eCG respectively. All does were naturally inseminated by four healthy fertile bucks. Laparotomy and embryo recovery were done for does on day six. The mean numbers of corpora lutea of nine groups were [2.8 +/- 4.34, 4.4 +/- 0.55, 2.8 +/- 1.10, 4.2 +/- 1.79, 6.8 +/- 2.39, 9.6 +/- 4.45, 6.8 +/- 5.81, 6.6 +/- 3.05 and 5.8 +/- 5.17] respectively. While mean of unovulated follicles were counted after laparotomy for nine groups respectively [3.4 +/- 1.14, 5.8 +/- 1.30, 7.0 +/- 2.35, 5.6 +/- 1.82, 6.8 +/- 2.05, 7.2 +/- 1.92, 9.8 +/- 4.71, 11.2 +/- 5.02 and 10.6 +/- 3.58]. On the other hand the percentage of normal embryos were 0, 15.0, 42.86, 42.11, 54.84, 66.67, 48.64, 38.0 and 45.24] of normal embryos. The present study indicates that an appropriate eCG dose would be 600-700 IU for a simple superovulation method in does pre-treated with Progestagen-impregnated vaginal sponges

comparative study of estrus induction with long-term and short term progesterone in ewes during non-breeding and breeding seasons

The objective of this trial was to compare the efficacy of long-term and short-term progesterone treatments on induction of estrus and pregnancy rate in Rahmani ewes during non-breeding and breeding seasons. Twenty-four animals were randomly grouped for two treatments with intravaginal progesterone device: Group A] during breeding season and Group B] during non-breeding season. Furthermore, both groups were subdivided into two treatments: Al and BI for short-treatment [7 days] and A2 and B2 for long-treatment [12 days]. The progesterone treatment was a vaginal sponge containing 40 mg medroxy progesterone acetate [MAP]. Equine chorionic gonadotrophin [eCG] and cloprostenol [PGF2 alpha] were intramuscularly administered to all ewes at the time of sponge removal. After the detection of estrus, the animals were hand-mated naturally. During the breeding season, mean percentages of estrus and pregnancy were 100% and 83.33%, respectively in both the short-term and long-term treated groups. During the non-breeding season, the average values of estrus were 100% in short-term and 100% for long-term progesterone treatment, but the pregnancy rates were 66.67 and 50.00% in the short- and long-term treated groups, respectively. The treatment with progesterone for short-term [7 days] was effective to induce estrus in sheep during both breeding and non-breeding seasons

Pregnancy rate in Egyptian buffaloes after synchronization of estrus with PGF2alpha or ovulation by ovsynch program [with 2 tables and 2 figures]

Postpartum in buffaloes plays a great role in delay of the cyclicity in buffaloes that seemed to be anestrous. So, the use of hormones to induct estrus or induct ovulation has an importance to increase pregnancy rate. The present study was designed to evaluate the efficacy of the two methods in inducing high pregnancy rate. The experimental animals [48 lactating buffaloes] were divided into three groups: PGF2alpha group 1[n=16]; Ovsynch group 2 [n=16] and control group [n=16]. Pregnancy rate was significantly [p<0.001]increased in group 2 than group 1 and control group due to higher level of progesterone and more accurate synchronization for ovulation., It is more suitable to use the regimen of ovsynch in postpartum buffaloes especially in multiparous females with good condition score