Occurrence of some microorganisms in relation to public health in kareish cheese [with 4 tables]

Fifty random samples of Kareish cheese were collected from local markets in Dakahlia Province, Egypt during spring of 2005 to be examined chemically and bacteriologically. The mean acidity%, moisture% and sodium chloride% of examined kareish cheese samples were 1.5, 61.2 and 5.4 respectively. While, the mean counts/g of examined samples for Escherichia coli, Staphylococcus aureus, Bacillus cereus, Clostridium perfringens and Enterococci were 3.8x10[3], 3.4x10[4], 1.8x10[4], 1.9x10[2] and 5.7x10[6] respectively. Yersinia enterocolitica was isolated from 4% of examined kareish cheese samples, while Salmonellae failed to be detected. Significant correlation was found between acidity%, moisture% sodium chloride% and different bacterial counts in kareish cheese. The public health importance of the isolated organisms as well as sanitary measures for improving the quality of kareish cheese were discussed

effect of pasteurella multocida lipopolysaccaride extract on the immune response of chickens vaccinated with inactivated new castle and egg drop syndrome vaccine [with 8 tables]

A total 120 chickens of L.B.S.L. of one day old were divided into 6 groups "20 chickens for each". The first three groups were vaccinated with oil emulsified inactivated NewCastle "ND", egg drop syndrome "EDS" vaccine and Pasteurella multosida [P .multosida] Lipopolysaccaride extract "LPS" in single doses which are 0.5 ml, 1/M respectively. The fourth group was vaccinated with bivalent oil adjuvant of ND and EDS. The fifth group was vaccinated with bivalent oil adjuvant vaccine of ND, EDS and LPS. The third and fifth groups received 2 doses of LPS to study its mitogenic effect on the vaccinated chickens. Blood sera were collected till 7 weeks post-vaccination for estimation of cell mediated immunity by lymphocyte transformation, phagocytic activity, phagocytic index and heterophils lymphocytes ratio. Humeral immunity was determined by IHA, HI, ELISA and challenge test. The obtaining data revealed that LPS protein complex of P. multocida had a mitogenic effect on chicken B lymphocytes vaccinated with combined NDV and EDS, enhancing the humeral antibodies and the protection against challenge of P. multocida and NDV were 80% and 90% respectively

Regio- and stereoselectivity in 1,3-dipolar cycloaddition reactions of 1- [phthalazin-3-yl] -3-oxidopyridinium betaine with 2 -1,3-dipolarophiles

Cycloaddition to 1-[phthalazin-3-yl]-3-oxidopyridinium betaine, across the 2, 6-positions of the pyridine ring with 2 pi-electron addends, gave substituted 8-azabicyclo [3.2.1] oct-3-en-2-ones. Structural and configurational assignments of the cycloadducts were deduced from PMR and IR spectral data