[Effect of combination of topical ointment of aqueous extract of grape seed [Vitis Vinifera] and Eucerin on wound healing in diabetic rats]

Background and Objective: Diabetic mellitus is one of the main health problems of diabetic subjects skin wound. This study was done to evaluate the effect of topical ointment produced from aqueous extract of grape seed [Vitis Vinifera] plus Eucerin on wound healing in diabetic rats

Methods: In this experimental study, 48 male Wistar rats were randomly allocated into control, first, second and third experimental groups. Animals in control group were received only buffer citrate, interaperitonally. Diabetes was induced by 55 mg/kg/bw of Streptozotocin in three experimental groups. Skin wound [2x3 cm[2]] was created in left side of the lumbar region in three experimental groups. Animals in experimental gourp 1 did not received any treatment. The wounds of animals in experimental group 2 were treated with topical ointment of Eucerin twice a day. The wounds of animals in experimental group 3 were treated with topical ointment of aqueous extract of grape seed plus Eucerin with a ratio of 3:1 for 21 days twice a day. Wound healing process was evaluated using macroscopic and histological method

Results: The mean area and percentage of wound after treatment was significantly reduced in experimental group 2 and 3 in compare to experimental group 1 [P<0.05]. The percentage of improvement wound healing was significantly increased in experimental groups 2 and 3 in compare to experimental group 1 [P<0.05]. Synthesis of collagen fibers was increased in experimental group 3 in compare to experimental group 2 and 1

Conclusion: Topical application of aqueous extract of grape seed with ointment of Eucerin accelerates skin wound healing in diabetic rats

Temperature requirements of some common forensically important blow and flesh flies [diptera] under laboratory conditions

The aim of his study was to determine development time and thermal requirements of three myiasis flies including Chrysomya albiceps, Lucilia sericata, and Sarcophaga sp. Rate of development [ROD] and accumulated degree day [ADD] of three important forensic flies in Iran, Chrysomya albiceps, Lucilia sericata, and Sarcophaga sp. by rearing individuals under a single constant temperature [28°C] was calculated using specific formula for four developmental events including egg hatching, larval stages, pupation, and eclosion. Rates of development decreased step by step as the flies grew from egg to larvae and then to adult stage; however, this rate was bigger for blowflies [C. albiceps and L. sericata] in comparison with the flesh fly Sarcophaga sp. Egg hatching, larval stages, and pupation took about one fourth and half of the time of the total pre-adult development time for all of the three species. In general, the flesh fly Sarcophaga sp. required more heat for development than the blowflies. The thermal constants [K] were 130-195, 148-222, and 221-323 degree-days [DD] for egg hatching to adult stages of C. albiceps, L. sericata, and Sarcophaga sp., respectively. This is the first report on thermal requirement of three forensic flies in Iran. The data of this study provide preliminary information for forensic entomologist to establish PMI in the area of study

[ effect of ethanol on growth and development of two-cell arrested mouse embryo]

Arresting in certain step of developing like two cell block, could be the reason of infertility in some couples. Evaluate the effect of ethanol on growth and development of mouse two-cell arrested embryo is aimed of this study. In this experimental study 4-6 weeks old female mice were coupled with male mice following superovulation. Positive vaginal plaque mice were killed 48 hour after HCG injection. Two cell embryos were collected in RPMI medium and cultured in M16 medium and divided in three groups. The 2[nd] and 3[rd] groups were exposed to 4[degree sign] [c] for 24 hour in order to delay and arrest for cleavage and developmental rate. The 2[nd] group [2[nd] control] were incubated immediately, while the 3[rd] group [experiment] were exposed to%0.1 Ethanole for 5 minutes and the 1[st] group [1[st]control] without any exposure to low temperature were incubated. The developmental rate of embryos exposed to low temperature [4[degree sign][c]] significantly decreased and induce, retardation and arrest [p=0.001]. There were not significant difference between the groups mean of cleavage rate, but the mean percent of degenerated embryos between groups have significant differences [p=0.045]. The mean percent of morulla were significantly different between groups [p=0.005]. The mean percent of blastocyst and hatched blastocyst after 120 hr evaluation have significant differences between others groups [p=0.014] [p=0.001]. Effect of%0.1 ethyl-alcohol on arrested two cell embryos can significantly increase the mean percent of morulla and development of blastocyst and hatching blastocyst stage in compare to control group, without any significant effect on cleavage rate