Antral gastrin cells in helicobacter pylori gastritis: quantitative immunohistochemical study

The number of antral gastrin cells [G cells] and their staining pattern was evaluated in 28 gastroscopic biopsies without evidence of Helicobacter-like organisms [HLOs], as a control group, and 42 gastroscopic biopsies with HLOs, as a test group. There was no significant difference in the number of G cells per gland in relation to the presence or absence of HLOs, degree of inflammation, type of gastritis and activity of inflammation [P >0.05]. The intensity of immunostaining was deeper in association with HLOs positive cases [++] than the negative group [+]. The intensity of G cell staining was not affected by the severity of HLOs. The intensity of staining was more in active than quiescent gastritis, specially when associated with HLOs. The degree of gastritis has direct effect on the intensity of staining of G cells, specially when associated with HLOs

Immunohistochemical and serological study of hepatitis B virus markers in chronic type B hepatitis

This study included 56 patients serologically positive for hepatitis B virus [HBV] markers. These were serologically screened for HBSAg, HBSAb, HBCAb, HBeAg and HBeAb. The inactive stage of chronic hepatitis involved 15 cases of chronic persistent hepatitis [CPH] and 6 cases of chronic lobular hepatitis [CLH]. The late sequelae of chronic hepatitis B virus included 8 cases of liver cirrhosis [LC] and 9 cases of hepatocellular carcinoma [HCC]. They were characterized by good percentage of positivity for HBeAb [76.3%] and hepatic cellular expression of HBSAg [78.9%] and/or HBSAb [57.9%] in the blood. The active form was presented by CAH and it was different from the inactive form by its positivity for HBeAg [76.3%], hepatic cellular expression of HBCAg [44.4%] and/or HBCAb [72.2%] in the blood. HBSAg was detected more in tissue [76.8%] than in serum [41.3%]. HBCAg was detected in 27.6% of biopsies and HBCAb was detected in 48.2%. HBCAg was correlated more with HBeAg specially in CAH. The correlation of HBSAb and anti-HBe was evident in cases of inactive chronic type B hepatitis