Celiac disease

A 17 year old female, presented with complaint of vomiting, weakness and lethargy since one week. She had history of leg deformities and protruded chest since age three. There was also a history of progressive weight loss and growth retardation.On examination, she was anemic [Hb. 3.2g/dl] with protruded eyes, short stature and pigeon chest deformity. Serum calcium and albumin were decreased and serum globulins were increased. Tissue Trans Glutaminase[TTG] antibodies were elevated. Duodenal biopsy revealed features consistent with celiac sprue

Lipid profile in ischemic heart disease in patients at Larkana

The lipid profile levels in ninty six [83 males of 13 females] ischaemic heart disease [IHD] patients aged 22 to 60 years divided into elder and younger age groups with upper and low income division alongwith 45 control subjects 611 matched for age and socioeconomic status were studied. Significantly elevated serum cholesterol, LDL cholesterol triglycerides, phospholipids and total lipids as well as significantly decreased HDL-cholesterol levels were found in IHD patients compared with control subjects. It was found that inspite of increased lipid profile with decreased HDL in IHD, there was no significant difference between the upper income and low income social classes, particularly in elder patients, but the difference was significant in upper and lower social classes in norm: III subjects

Some properties of crude phospholipase C enzyme from Oxystelma Esculantum

Phospholipase C activity was demonstrated from axystelma esculantum root tuber. The optimum enzyme activity was found at pH 5.5 and temperature 30°C. Enzyme activity was stimulated with Triton x - 100 [1mM] 25%, sodium deoxycholate [1mM] 65%, cobalt chloride [5mM] 162.5% and Zinc chloride [10mM] 180%. Enzyme activity showed 100% inhibition with EDTA [1mM] and Calcium chloride [10mM]. Oxystelma esculantum crude Phospholipase C enzyme activity was found to be heat labile