Isolation, characterization and transduction of canine bone marrow-derived mesenchymal stem cells [cBM-MSCs]

Stem cell therapy in small animal medicine is still in its infancy and few in vitro and in vivo research projects regarding animal Mesenchymal Stem Cells [MSCs] have been carried out. On the other hand, Cell tracking is the first step of the cell-based therapies and is essential to recognize cell fate post transplantation. The aim of this study was to isolate, characterize, and transduce cBM-MSCs. Canine Bone Marrow-derived Mesenchymal Stem Cells [cBMMSCs] were isolated from bone marrow of dogs and characterized based on morphology, differentiation capacities, and surface marker expressions. For the first time, we labeled cBM-MSCs by GFP-encoding lentiviral vector to track them. cBM-MSCs were successfully isolated and proliferated. Morphologically, these cells were similar to other MSCs from other sources and species and were able to differentiate into osteocytes and adipocytes. cBM-MSCs expressed surface marker CD44 but were not able to express CD34. Approximately, 70% of cells efficaciously expressed GFP after labeling; We found that GFP labeling is an easy and effective technique to track transplanted cBMMSCs. Our results also provide fundamental information about canine BM-MSCs in order to use in veterinary medicine

Prevalence and risk indicators of periodontal disease among high-school students in Tehran

To identify the prevalence and risk indicators of periodontal disease in high-school students in Tehran, Islamic Republic of Iran, the periodontal condition of 867 students aged 15-19 years was assessed using the community periodontal index of treatment needs [CPITN]. The results showed that 88.7% of these students had less than perfect periodontal health. Multiple regression analysis revealed that sex, parents' educational level, frequency of toothbrushing and flossing, preventive dental visits and presence of extracted teeth were significant risk indicators for periodontal disease. School-based oral health promotion and prevention programmes are needed

Histologic comparison of Bio-Oss and Neo-Oss in bone regeneration of experimental defects on rabbit calvarium

Several biomaterials have been presented for regeneration of intraosseous defects and Bio-Oss is one of the most accepted materials in this field. Neo-Oss may be the first and only regenerative material made in Iran which few studies have been published about it. The aim of this study was the histologic evaluation of Neo-Oss in comparison with Bio-Oss in experimental rabbit calvarial defects. This was an interventional in vivo study. After elevating muccoperiosteal flap 18 standard defects [3X6mm] in frontal and parietal bone were made by round bur on the calvarium of 6 New Zealand rabbits. Defects were randomly assigned to one of the following treatments: 1-No graft [control], 2-Bio-Oss graft 3-Neo-Oss graft. After 4 weeks the animals were killed and histologic sections prepared. The studied variables consisted of inflammation, type of regenerated bone, thickness of bony trabeculation and foreign body reaction. Data were analyzed by Friedman test with p<0.05 as the limit of significance. The results showed that Bio-Oss was effective for bone regeneration. Inflammation [P= 0.026] and foreign body reaction [P= 0.002] in Neo-Oss was significantly more than Bio-Oss group. Thickness of terabecula in Neo-Oss group [66.7% thin] was significantly different from Bio-Oss [100% thick] and control group [50% thin 50% thick] [P= 0.006]. In Neo-Oss group, granulation tissue containing giant cells was observed and the rate of resorption during 4 weeks was high. Based on the results of this study, the healing capacity of Neo-Oss in comparison with Bio-Oss is not acceptable