RESUMO
Out of 35 rectal swabs [23] from calves and [12] from sheep. E.coli isolated from calves by about [69.56%] and [66.66%]. And more over 31 cloacal swabs from chicken 6 [19.35%] E.coli were isolated with total percent of [45.5%].Eight E.coli isolates from cattle were O grouped and 8 were unidentified. The O grouping revealed of isolation of different [6] O serogroups [O6, O1, O25, O18, O153and O119]. Serogrouping of 5 E.coli isolates from sheep resulted in 3 different serogroups [O169, O27 and O8] and 3 samples were ungrouped. Serogrouping of 5 E.coli isolates from chicken resulted in different 3 serogroups [O27, O169, and O20]. The result of invitro sensitivity testing of 30 isolates of E.coli against 8 different antibiotics by using disc diffusion method indicated that nearly all isolates of all animals isolated were sensitive to ciprofloxacin, garamycin and levofloxacin with percent of [3.33%], [6.66%] and [10.0%] respectively. High resistance were observed to both streptomycin and colistin .aspartame antibiotic with rate of [56.6%] and [66.6%] respectively. The present study also showed that the presence of multiple resistances. The results of polymerase chain reaction [PCR] using bacterial DNA with a percent of 7.69%. indicated that the aad A1 gene responsible for resistance of E.coli to streptomycin, and it found in 7.69% of the isolates but the other isolates were negative for aadA1 gene